Ser7
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Home > Phosphorylation Site Page: > Ser7  -  NUMB (human)

Site Information
_MNKLRQsFRRKkDV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 487518

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 2 ) , mass spectrometry ( 1 ) , mutation of modification site ( 2 ) , phospho-antibody ( 2 ) , western blotting ( 2 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
PKCI (human) ( 2 )
Kinases, in vitro:
PKCI (human) ( 2 )
Treatments:
bisindolylmaleimide ( 2 ) , calyculin_A ( 2 )

Downstream Regulation
Effects of modification on NUMB:
intracellular localization ( 2 )
Effects of modification on biological processes:
cell motility, induced ( 2 ) , endocytosis, induced ( 2 )
Induce interaction with:
PAR3 (human) ( 2 )
Inhibit interaction with:
AP2A1 (human) ( 2 ) , ITGB1 (human) ( 2 )

References 

1

Zhou J (2008) CST Curation Set: 5314; Year: 2008; Biosample/Treatment: cell line, Jurkat/TPA; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (K/R)XpSX(K/R)Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser) PKC Substrate Antibody Cat#: 2261, PTMScan(R) Phospho-PKC Substrate Motif (K/RXpSXK/R) Immunoaffinity Beads Cat#: 1985
Curated Info

2

Nishimura T, Kaibuchi K (2007) Numb controls integrin endocytosis for directional cell migration with aPKC and PAR-3. Dev Cell 13, 15-28
17609107   Curated Info