Tyr332
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Home > Phosphorylation Site Page: > Tyr332  -  PKCD (rat)

Site Information
IPDNNGTyGKIWEGS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450077

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 7 ) , mutation of modification site ( 1 , 2 , 4 , 5 , 6 , 7 ) , phospho-antibody ( 1 , 2 , 3 , 5 , 6 , 7 ) , western blotting ( 1 , 2 , 3 , 5 , 7 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Kinases, in vitro:
EGFR (human) ( 2 ) , Src (human) ( 1 ) , Src (rat) ( 3 )
Treatments:
AG1478 ( 2 ) , ceramide ( 5 ) , EGF ( 2 ) , H2O2 ( 1 , 2 , 7 ) , herbimycin_A ( 5 ) , PD153035 ( 2 ) , PP1 ( 1 ) , PP2 ( 5 )

Downstream Regulation
Effects of modification on PKCD:
enzymatic activity, induced ( 1 , 5 ) , molecular association, regulation ( 2 , 6 )
Effects of modification on biological processes:
apoptosis, induced ( 5 )
Induce interaction with:
SHC1 (human) ( 2 , 6 )

References 

1

Sumandea MP, et al. (2008) Tyrosine phosphorylation modifies protein kinase C delta-dependent phosphorylation of cardiac troponin I. J Biol Chem 283, 22680-9
18550549   Curated Info

2

Morita M, et al. (2008) Epidermal Growth Factor Receptor Phosphorylates Protein Kinase C {delta} at Tyr332 to form a Trimeric Complex with p66Shc in the H2O2-stimulated Cells. J Biochem 143, 31-8
17956904   Curated Info

3

Rybin VO, et al. (2007) Protein kinase Cepsilon (PKCepsilon) and Src control PKCdelta activation loop phosphorylation in cardiomyocytes. J Biol Chem 282, 23631-8
17569658   Curated Info

4

Kuriyama M, et al. (2004) Activation and translocation of PKCdelta is necessary for VEGF-induced ERK activation through KDR in HEK293T cells. Biochem Biophys Res Commun 325, 843-51
15541367   Curated Info

5

Kajimoto T, et al. (2004) Ceramide-induced apoptosis by translocation, phosphorylation, and activation of protein kinase Cdelta in the Golgi complex. J Biol Chem 279, 12668-76
14715667   Curated Info

6

Leitges M, et al. (2002) Protein kinase C-delta is a negative regulator of antigen-induced mast cell degranulation. Mol Cell Biol 22, 3970-80
12024011   Curated Info

7

Konishi H, et al. (2001) Phosphorylation sites of protein kinase C delta in H2O2-treated cells and its activation by tyrosine kinase in vitro. Proc Natl Acad Sci U S A 98, 6587-92
11381116   Curated Info