Ser33
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Home > Phosphorylation Site Page: > Ser33  -  CTNNB1 (human)

Site Information
QQQsyLDsGIHsGAT   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447487

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 43 ) , immunoprecipitation ( 3 , 4 , 9 , 15 , 26 , 28 , 29 ) , mass spectrometry ( 18 , 21 , 31 , 37 , 47 ) , mutation of modification site ( 6 , 9 , 15 , 19 , 24 , 27 , 32 , 33 , 34 , 35 , 40 , 42 , 43 , 45 , 46 , 47 , 49 , 51 ) , phospho-antibody ( 2 , 3 , 4 , 5 , 6 , 9 , 11 , 20 , 22 , 23 , 24 , 25 , 26 , 28 , 29 , 30 , 31 , 33 , 34 , 36 , 38 , 39 , 40 , 41 , 42 , 44 , 45 , 46 , 47 , 48 , 49 ) , western blotting ( 2 , 3 , 4 , 5 , 6 , 9 , 11 , 15 , 20 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 33 , 34 , 38 , 41 , 42 , 44 , 45 , 46 , 47 , 48 , 49 )
Disease tissue studied:
bone cancer ( 24 ) , breast cancer ( 6 , 28 , 46 ) , colorectal cancer ( 15 , 22 , 25 , 30 , 31 , 42 , 45 ) , colorectal carcinoma ( 15 , 22 , 25 , 30 , 31 , 42 , 45 ) , gastric cancer ( 18 , 29 ) , gastric carcinoma ( 18 , 29 ) , kidney cancer ( 23 ) , leukemia ( 34 ) , chronic myelogenous leukemia ( 34 ) , lung cancer ( 21 ) , non-small cell lung cancer ( 21 ) , non-small cell squamous cell lung carcinoma ( 21 ) , ovarian cancer ( 37 ) , pancreatic cancer ( 5 ) , pancreatic carcinoma ( 5 ) , melanoma skin cancer ( 11 , 37 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 2 , 3 , 38 , 39 , 47 ) , 293T (epithelial) ( 15 , 22 , 30 , 45 , 46 , 49 , 50 ) , 3Y1 (fibroblast) ( 33 ) , 4T1 (breast cell) ( 28 ) , A375 (melanocyte) ( 11 ) , A431 (epithelial) ( 26 ) , A498 (renal) [SFRP2 (human), transfection] ( 23 ) , AGS (gastric) ( 29 ) , BC-1 (B lymphocyte) ( 34 ) , C57MG (epithelial) ( 27 ) , Caco-2 (intestinal) ( 15 ) , Capan1 (pancreatic) ( 5 ) , CD18/HPAF (pancreatic) ( 5 ) , CHO (fibroblast) ( 49 ) , COS (fibroblast) ( 32 , 33 , 38 , 43 , 46 ) , COV413 (ovarian) ( 37 ) , DLD1 (intestinal) ( 30 ) , DM331 (melanocyte) ( 37 ) , endothelial-vein ( 44 ) , HCT116 (intestinal) ( 22 , 31 , 45 ) , HeLa (cervical) ( 3 , 4 , 47 , 50 ) , HFFF-2 (fibroblast) ( 33 ) , HT-29 (intestinal) ( 36 , 45 ) , Jurkat (T lymphocyte) ( 41 , 47 ) , keratinocyte ( 19 ) , KU812 (myeloid) ( 34 ) , L cells (fibroblast) ( 47 ) , L3.6p1 (pancreatic) ( 5 ) , Ls174T (intestinal) ( 42 ) , MCF-7 (breast cell) ( 6 , 28 ) , MDCK (epithelial) ( 40 , 45 ) , MEF (fibroblast) [axin 2 (mouse), homozygous knockout] ( 24 ) , mesangial ( 20 ) , MKN-45 (gastric) ( 18 ) , MKN-74 (gastric) ( 29 ) , NCI-H520 (squamous) ( 21 ) , oocyte ( 48 ) , Rat2 (fibroblast) ( 48 ) , SKBr3 (breast cell) ( 46 ) , SLM2 (melanocyte) ( 37 ) , SNU16Ad (gastric) ( 29 ) , SUM159 (breast cell) ( 6 ) , SW48 (intestinal) ( 51 ) , SW480 (intestinal) ( 22 , 25 , 45 ) , SW620 (intestinal) ( 22 ) , T lymphocyte-blood ( 35 ) , U2OS (bone cell) ( 24 )

Upstream Regulation
Regulatory protein:
AurA (human) ( 29 ) , axin 1 (human) ( 47 ) , CDH2 (human) ( 45 ) , CTNNB1 (human) ( 27 ) , DDX5 (human) ( 36 ) , DKK1 (human) ( 20 ) , GSKIP (human) ( 3 ) , Merlin (human) ( 6 ) , PPP2R2A (human) ( 5 ) , SFRP2 (human) ( 23 ) , WNT5A (human) ( 28 )
Putative in vivo kinases:
GSK3B (human) ( 48 ) , IKKA (human) ( 43 ) , JNK1 (human) ( 26 )
Kinases, in vitro:
GSK3A (human) ( 48 ) , GSK3B (human) ( 30 , 39 , 47 ) , JNK1 (human) ( 26 )
Phosphatases, in vitro:
PPP2CA (human) ( 30 )
Treatments:
anti-FGF19 ( 31 ) , calyculin_A ( 22 ) , cAMP_analog ( 39 ) , CK1-7 ( 47 ) , colforsin ( 38 ) , CTGF ( 20 ) , H-89 ( 47 ) , KY1022 ( 2 ) , lithium ( 22 , 45 , 47 , 48 ) , LY2090314 ( 11 ) , MG132 ( 3 , 31 , 45 ) , okadaic_acid ( 26 ) , SB216763 ( 34 ) , SP600125 ( 26 ) , Wnt ( 48 ) , Wnt3a ( 24 , 47 )

Downstream Regulation
Effects of modification on CTNNB1:
activity, inhibited ( 35 ) , intracellular localization ( 25 , 29 , 32 , 36 ) , molecular association, regulation ( 26 ) , protein degradation ( 29 , 30 , 47 ) , ubiquitination ( 40 )
Effects of modification on biological processes:
cell adhesion, altered ( 26 ) , cell growth, altered ( 29 , 46 ) , cytoskeletal reorganization ( 24 ) , transcription, altered ( 29 , 35 ) , transcription, inhibited ( 15 , 49 )
Induce interaction with:
CBLL1 (mouse) ( 26 )

Disease / Diagnostics Relevance
Relevant diseases:
colorectal cancer ( 48 ) , ovarian cancer ( 37 ) , melanoma skin cancer ( 37 )

References 

1

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2

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3

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4

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7

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8

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9

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10

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11

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12

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13

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14

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15

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16

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17

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18

Moritz A (2011) CST Curation Set: 12371; Year: 2011; Biosample/Treatment: cell line, MKN-45/untreated; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

19

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20

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21

Rikova K (2011) CST Curation Set: 10911; Year: 2011; Biosample/Treatment: cell line, NCI-H520/untreated; Disease: non-small cell squamous cell lung carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

22

Kwon IK, et al. (2010) PKG inhibits TCF signaling in colon cancer cells by blocking beta-catenin expression and activating FOXO4. Oncogene 29, 3423-34
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23

Yamamura S, et al. (2010) Oncogenic functions of secreted Frizzled-related protein 2 in human renal cancer. Mol Cancer Ther 9, 1680-7
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24

Hadjihannas MV, Brückner M, Behrens J (2010) Conductin/axin2 and Wnt signalling regulates centrosome cohesion. EMBO Rep 11, 317-24
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25

Maher MT, et al. (2010) Beta-catenin phosphorylated at serine 45 is spatially uncoupled from beta-catenin phosphorylated in the GSK3 domain: implications for signaling. PLoS One 5, e10184
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26

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27

Wu H, Symes K, Seldin DC, Dominguez I (2009) Threonine 393 of beta-catenin regulates interaction with Axin. J Cell Biochem 108, 52-63
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28

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29

Dar AA, Belkhiri A, El-Rifai W (2009) The aurora kinase A regulates GSK-3beta in gastric cancer cells. Oncogene 28, 866-75
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30

Su Y, et al. (2008) APC is essential for targeting phosphorylated beta-catenin to the SCFbeta-TrCP ubiquitin ligase. Mol Cell 32, 652-61
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31

Pai R, et al. (2008) Inhibition of fibroblast growth factor 19 reduces tumor growth by modulating beta-catenin signaling. Cancer Res 68, 5086-95
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32

Li FQ, Mofunanya A, Harris K, Takemaru K (2008) Chibby cooperates with 14-3-3 to regulate beta-catenin subcellular distribution and signaling activity. J Cell Biol 181, 1141-54
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33

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34

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35

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36

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37

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38

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39

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40

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41

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42

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43

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44

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45

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46

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47

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48

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49

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50

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51

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