Tyr222

Site Information
GGPEPGPyAQPSINT SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 447951

In vivo Characterization
Methods used to characterize site in vivo:	mutation of modification site ( 1 , 3 ) , phospho-antibody ( 1 , 3 ) , western blotting ( 1 , 3 )
Disease tissue studied:	adrenal cancer ( 3 ) , pheochromocytoma ( 3 )
Relevant cell line - cell type - tissue:	HEK293T (epithelial) ( 1 , 3 ) , MEF (fibroblast) ( 3 ) , PC-12 (chromaffin) ( 3 )

Downstream Regulation
Effects of modification on CRK:	molecular association, regulation ( 1 , 3 ) , protein conformation ( 1 )
Effects of modification on biological processes:	cell adhesion, altered ( 3 ) , cell differentiation, altered ( 3 )
Inhibit interaction with:	Abl (human) ( 1 ) , Abl iso2 (human) ( 3 ) , PXN (chicken) ( 3 )

References
1	Sarkar P, et al. (2007) Proline cis-trans isomerization controls autoinhibition of a signaling protein. Mol Cell 25, 413-26 17289588 Curated Info
2	Hu H, Bliss JM, Wang Y, Colicelli J (2005) RIN1 is an ABL tyrosine kinase activator and a regulator of epithelial-cell adhesion and migration. Curr Biol 15, 815-23 15886098 Curated Info
3	Escalante M, et al. (2000) Phosphorylation of c-Crk II on the negative regulatory Tyr222 mediates nerve growth factor-induced cell spreading and morphogenesis. J Biol Chem 275, 24787-97 10825157 Curated Info