Thr287
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Home > Phosphorylation Site Page: > Thr287  -  CAMK2B (rat)

Site Information
sMMHRQEtVECLkkF   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450048

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 9 , 11 , 12 , 13 ) , immunoprecipitation ( 2 ) , mass spectrometry ( 5 ) , mass spectrometry (in vitro) ( 1 , 8 ) , mutation of modification site ( 6 , 7 ) , phospho-antibody ( 1 , 2 , 3 , 10 , 12 ) , phosphoamino acid analysis ( 9 , 11 ) , phosphopeptide mapping ( 9 , 11 , 12 , 13 ) , western blotting ( 2 , 3 , 7 , 12 )
Relevant cell line - cell type - tissue:
'brain, cerebellum' ( 5 ) , 'brain, cerebral cortex' ( 5 ) , 'brain, hippocampus' ( 12 , 13 ) , 'neuron, cerebellar granule'-brain ( 6 ) , 'neuron, hippocampal'-brain ( 1 ) , 'neuron, hippocampal, CA1 pyramidal'-brain ( 9 , 11 ) , 293 (epithelial) ( 7 ) , COS (fibroblast) ( 12 ) , dorsal root ganglia ( 10 ) , neuron-'brain, hippocampus' ( 7 ) , oligodendrocyte precursor ( 3 )

Upstream Regulation
Putative in vivo kinases:
CAMK2B (rat) ( 11 )
Kinases, in vitro:
CAMK2A (rat) ( 8 ) , CAMK2B (rat) ( 1 , 8 , 11 )
Treatments:
Ca(2+) ( 9 , 11 ) , capsaicin ( 10 ) , DHPG ( 2 )

Downstream Regulation
Effects of modification on CAMK2B:
activity, induced ( 6 ) , enzymatic activity, induced ( 11 ) , intracellular localization ( 7 ) , molecular association, regulation ( 2 , 7 , 9 ) , phosphorylation ( 9 )
Effects of modification on biological processes:
cell growth, inhibited ( 6 )
Induce interaction with:
CAMK2A (rat) ( 9 , 11 ) , CAMK2B (rat) ( 9 , 11 ) , MARCKS (rat) ( 9 ) , RPT6 (rat) ( 7 ) , SYN1 (rat) ( 9 )
Inhibit interaction with:
ACTN4 (human) ( 2 )

Disease / Diagnostics Relevance
Relevant diseases:
type 2 diabetes ( 4 )

References 

1

Kim K, et al. (2015) A Temporary Gating of Actin Remodeling during Synaptic Plasticity Consists of the Interplay between the Kinase and Structural Functions of CaMKII. Neuron 87, 813-26
26291163   Curated Info

2

Kalinowska M, et al. (2015) Actinin-4 Governs Dendritic Spine Dynamics and Promotes Their Remodeling by Metabotropic Glutamate Receptors. J Biol Chem 290, 15909-20
25944910   Curated Info

3

Martinez-Lozada Z, et al. (2014) Activation of sodium-dependent glutamate transporters regulates the morphological aspects of oligodendrocyte maturation via signaling through calcium/calmodulin-dependent kinase IIβ's actin-binding/-stabilizing domain. Glia 62, 1543-58
24866099   Curated Info

4

Liao MH, et al. (2013) The Disturbance of Hippocampal CaMKII/PKA/PKC Phosphorylation in Early Experimental Diabetes Mellitus. CNS Neurosci Ther 19, 329-36
23490331   Curated Info

5

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

6

Puram SV, et al. (2011) A CaMKIIβ signaling pathway at the centrosome regulates dendrite patterning in the brain. Nat Neurosci 14, 973-83
21725312   Curated Info

7

Bingol B, et al. (2010) Autophosphorylated CaMKIIalpha acts as a scaffold to recruit proteasomes to dendritic spines. Cell 140, 567-78
20178748   Curated Info

8

Dosemeci A, Jaffe H (2010) Regulation of phosphorylation at the postsynaptic density during different activity states of Ca2+/calmodulin-dependent protein kinase II. Biochem Biophys Res Commun 391, 78-84
19896464   Curated Info

9

Avramovich-Tirosh Y, et al. (2007) Therapeutic targets and potential of the novel brain- permeable multifunctional iron chelator-monoamine oxidase inhibitor drug, M-30, for the treatment of Alzheimer's disease. J Neurochem 100, 490-502
17144902   Curated Info

10

Larsson M, Broman J (2006) Pathway-specific bidirectional regulation of Ca2+/calmodulin-dependent protein kinase II at spinal nociceptive synapses after acute noxious stimulation. J Neurosci 26, 4198-205
16624940   Curated Info

11

Scholz WK, Palfrey HC (1998) Activation of Ca2+/calmodulin-dependent protein kinase II by extracellular calcium in cultured hippocampal pyramidal neurons. J Neurochem 71, 580-91
9681448   Curated Info

12

Patton BL, Molloy SS, Kennedy MB (1993) Autophosphorylation of type II CaM kinase in hippocampal neurons: localization of phospho- and dephosphokinase with complementary phosphorylation site-specific antibodies. Mol Biol Cell 4, 159-72
8443414   Curated Info

13

Molloy SS, Kennedy MB (1991) Autophosphorylation of type II Ca2+/calmodulin-dependent protein kinase in cultures of postnatal rat hippocampal slices. Proc Natl Acad Sci U S A 88, 4756-60
1647015   Curated Info