Ser23
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Home > Phosphorylation Site Page: > Ser23  -  TNNI3 (rat)

Site Information
PAPVRRRssANYRAY   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448423

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 5 , 13 , 14 ) , mass spectrometry ( 4 , 5 ) , mutation of modification site ( 1 , 2 , 5 , 8 , 10 , 11 , 13 ) , phospho-antibody ( 5 , 6 , 7 , 8 , 9 , 11 , 12 ) , phosphopeptide mapping ( 14 ) , western blotting ( 1 , 2 , 5 , 6 , 7 , 8 , 9 , 11 )
Disease tissue studied:
LVR ( 6 ) , MI ( 6 )
Relevant cell line - cell type - tissue:
E.coli (bacterial) ( 5 ) , heart ( 4 , 5 , 10 ) , intestine ( 4 ) , lung ( 4 ) , myocardium ( 6 ) , myocyte-heart ( 1 , 2 , 5 , 7 , 8 , 9 , 11 , 12 , 13 , 14 )

Upstream Regulation
Regulatory protein:
PMCA4 iso2 (human) ( 7 ) , PRKD1 (mouse) ( 9 )
Putative in vivo kinases:
PKCA (rat) ( 13 ) , PRKD1 (mouse) ( 9 )
Kinases, in vitro:
PKACA (human) ( 8 , 9 ) , PKCA (human) ( 8 ) , PKCB iso2 (human) ( 8 ) , PKCD (rat) ( 8 ) , PRKD1 (human) ( 8 , 12 ) , PRKD1 (mouse) ( 9 )
Treatments:
bisindolylmaleimide ( 11 , 13 ) , calyculin_A ( 2 ) , chelerythrine ( 13 ) , dobutamine ( 11 ) , endothelin ( 9 , 11 , 13 ) , H-89 ( 13 ) , isoproterenol ( 7 , 9 ) , PD98059 ( 13 ) , phenylephrine ( 11 ) , phorbol_ester ( 11 , 14 ) , propranolol ( 11 ) , SB203580 ( 13 ) , SP600125 ( 13 )

Downstream Regulation
Effects of modification on TNNI3:
activity, inhibited ( 11 ) , protein conformation ( 2 )
Effects of modification on biological processes:
cell motility, altered ( 10 ) , cytoskeletal reorganization ( 8 )

References 

1

Lang SE, et al. (2017) Secondary phosphorylation in myocytes expressing FLAG-tagged and non-tagged phospho-mimetic cardiac troponin I. Data Brief 15, 562-566
29071293   Curated Info

2

Lang SE, et al. (2017) Functional communication between PKC-targeted cardiac troponin I phosphorylation sites. Arch Biochem Biophys 627, 1-9
28587770   Curated Info

3

Wang H, et al. (2013) TNNI3K is a novel mediator of myofilament function and phosphorylates cardiac troponin I. Braz J Med Biol Res 46, 128-37
23369981   Curated Info

4

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

5

Sancho Solis R, Ge Y, Walker JW (2011) A preferred AMPK phosphorylation site adjacent to the inhibitory loop of cardiac and skeletal troponin I. Protein Sci 20, 894-907
21416543   Curated Info

6

Dubois E, et al. (2011) Decreased serine207 phosphorylation of troponin T as a biomarker for left ventricular remodelling after myocardial infarction. Eur Heart J 32, 115-23
20418543   Curated Info

7

Mohamed TM, et al. (2009) Specific role of neuronal nitric-oxide synthase when tethered to the plasma membrane calcium pump in regulating the beta-adrenergic signal in the myocardium. J Biol Chem 284, 12091-8
19278978   Curated Info

8

Sumandea MP, et al. (2008) Tyrosine phosphorylation modifies protein kinase C delta-dependent phosphorylation of cardiac troponin I. J Biol Chem 283, 22680-9
18550549   Curated Info

9

Cuello F, et al. (2007) Protein kinase D selectively targets cardiac troponin I and regulates myofilament Ca2+ sensitivity in ventricular myocytes. Circ Res 100, 864-73
17322173   Curated Info

10

Bilchick KC, et al. (2007) Heart failure-associated alterations in troponin I phosphorylation impair ventricular relaxation-afterload and force-frequency responses and systolic function. Am J Physiol Heart Circ Physiol 292, H318-25
16936010   Curated Info

11

Westfall MV, Lee AM, Robinson DA (2005) Differential contribution of troponin I phosphorylation sites to the endothelin-modulated contractile response. J Biol Chem 280, 41324-31
16236710   Curated Info

12

Haworth RS, et al. (2004) Protein kinase D is a novel mediator of cardiac troponin I phosphorylation and regulates myofilament function. Circ Res 95, 1091-9
15514163   Curated Info

13

Westfall MV, Borton AR (2003) Role of troponin I phosphorylation in protein kinase C-mediated enhanced contractile performance of rat myocytes. J Biol Chem 278, 33694-700
12815045   Curated Info

14

Jideama NM, et al. (1996) Phosphorylation specificities of protein kinase C isozymes for bovine cardiac troponin I and troponin T and sites within these proteins and regulation of myofilament properties. J Biol Chem 271, 23277-83
8798526   Curated Info