Ser635
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Home > Phosphorylation Site Page: > Ser635  -  eNOS (cow)

Site Information
WRRKRKEsSNTDSAG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448637

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 11 ) , mutation of modification site ( 5 , 10 , 11 , 13 ) , phospho-antibody ( 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 ) , phosphopeptide mapping ( 11 ) , western blotting ( 3 , 4 , 5 , 7 , 9 , 11 , 12 , 13 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 3 ) , BAE ( 4 , 11 ) , BAEC (endothelial) ( 1 , 3 , 5 , 10 , 12 ) , BLMVEC (endothelial) ( 9 ) , COS (fibroblast) ( 5 , 10 , 13 ) , endothelial-aorta ( 6 , 7 , 8 )

Upstream Regulation
Regulatory protein:
eNOS (cow) ( 5 ) , PTEN (human) ( 13 )
Treatments:
angiopoietin-1 ( 1 ) , apoA-I ( 8 ) , ATP ( 10 , 11 ) , BAPTA-AM ( 11 ) , bradykinin ( 11 ) , calyculin_A ( 5 ) , cAMP_analog ( 12 ) , fluid_shear_stress ( 12 ) , H-89 ( 12 ) , HDL ( 8 ) , IBMX ( 11 ) , ionomycin ( 5 ) , KT5720 ( 7 , 11 ) , lactacystin ( 3 ) , LDL ( 8 ) , lovastatin ( 7 ) , LY294002 ( 11 , 12 ) , MG132 ( 3 ) , mPKI ( 12 ) , pravastatin ( 7 ) , quercetin ( 4 ) , vanadate ( 9 ) , VEGF ( 1 , 10 , 11 , 12 ) , wortmannin ( 11 , 12 )

Downstream Regulation
Effects of modification on eNOS:
acetylation ( 2 ) , enzymatic activity, induced ( 5 , 10 , 11 ) , phosphorylation ( 10 )

References 

1

Chidiac R, et al. (2016) Comparative Phosphoproteomics Analysis of VEGF and Angiopoietin-1 Signaling Reveals ZO-1 as a Critical Regulator of Endothelial Cell Proliferation. Mol Cell Proteomics 15, 1511-25
26846344   Curated Info

2

Chen Z, et al. (2010) Shear stress, SIRT1, and vascular homeostasis. Proc Natl Acad Sci U S A 107, 10268-73
20479254   Curated Info

3

Wei Q, Xia Y (2006) Proteasome inhibition down-regulates endothelial nitric-oxide synthase phosphorylation and function. J Biol Chem 281, 21652-9
16737962   Curated Info

4

Jackson SJ, Venema RC (2006) Quercetin inhibits eNOS, microtubule polymerization, and mitotic progression in bovine aortic endothelial cells. J Nutr 136, 1178-84
16614401   Curated Info

5

Church JE, Fulton D (2006) Differences in eNOS activity because of subcellular localization are dictated by phosphorylation state rather than the local calcium environment. J Biol Chem 281, 1477-88
16257964   Curated Info

6

Li C, et al. (2005) Interaction of the endothelial nitric oxide synthase with the CAT-1 arginine transporter enhances NO release by a mechanism not involving arginine transport. Biochem J 386, 567-74
15743275   Curated Info

7

Harris MB, et al. (2004) Acute activation and phosphorylation of endothelial nitric oxide synthase by HMG-CoA reductase inhibitors. Am J Physiol Heart Circ Physiol 287, H560-6
15087285   Curated Info

8

Drew BG, et al. (2004) High-density lipoprotein and apolipoprotein AI increase endothelial NO synthase activity by protein association and multisite phosphorylation. Proc Natl Acad Sci U S A 101, 6999-7004
15107497   Curated Info

9

Papapetropoulos A, et al. (2004) Vanadate is a potent activator of endothelial nitric-oxide synthase: evidence for the role of the serine/threonine kinase Akt and the 90-kDa heat shock protein. Mol Pharmacol 65, 407-15
14742683   Curated Info

10

Bauer PM, et al. (2003) Compensatory phosphorylation and protein-protein interactions revealed by loss of function and gain of function mutants of multiple serine phosphorylation sites in endothelial nitric-oxide synthase. J Biol Chem 278, 14841-9
12591925   Curated Info

11

Michell BJ, et al. (2002) Identification of regulatory sites of phosphorylation of the bovine endothelial nitric-oxide synthase at serine 617 and serine 635. J Biol Chem 277, 42344-51
12171920   Curated Info

12

Boo YC, et al. (2002) Shear stress stimulates phosphorylation of eNOS at Ser(635) by a protein kinase A-dependent mechanism. Am J Physiol Heart Circ Physiol 283, H1819-28
12384459   Curated Info

13

Church JE, et al. Inhibition of endothelial nitric oxide synthase by the lipid phosphatase PTEN. Vascul Pharmacol 52, 191-8
19962452   Curated Info