Ser104

Site Information
KKEEsEEsEDDMGFG SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 456061

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 , 7 )
Relevant cell line - cell type - tissue:	'brain, brainstem' ( 3 ) , 'brain, cerebellum' ( 3 ) , 'brain, cerebral cortex' ( 3 ) , 'kidney, inner medulla' ( 5 , 6 ) , adipose tissue ( 3 ) , blood ( 3 ) , heart ( 3 ) , IEC-6 (epithelial) ( 1 ) , intestine ( 3 ) , kidney ( 3 ) , liver ( 3 , 4 , 7 ) , lung ( 3 ) , muscle ( 3 ) , pancreas ( 3 ) , PC-12 (chromaffin) [TrkA (rat), transfection] ( 2 ) , PC-12 (chromaffin) ( 2 ) , spleen ( 3 ) , stomach ( 3 ) , testis ( 3 ) , thymus ( 3 )

In vivo Characterization

Methods used to characterize site in vivo:

mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 , 7 )

Relevant cell line - cell type - tissue:

'brain, brainstem' ( 3 ) , 'brain, cerebellum' ( 3 ) , 'brain, cerebral cortex' ( 3 ) , 'kidney, inner medulla' ( 5 , 6 ) , adipose tissue ( 3 ) , blood ( 3 ) , heart ( 3 ) , IEC-6 (epithelial) ( 1 ) , intestine ( 3 ) , kidney ( 3 ) , liver ( 3 , 4 , 7 ) , lung ( 3 ) , muscle ( 3 ) , pancreas ( 3 ) , PC-12 (chromaffin) [TrkA (rat), transfection] ( 2 ) , PC-12 (chromaffin) ( 2 ) , spleen ( 3 ) , stomach ( 3 ) , testis ( 3 ) , thymus ( 3 )

Upstream Regulation
Treatments:	PD184352 ( 1 ) , PDGF ( 2 ) , serum ( 1 )

References
1	Courcelles M, et al. (2013) Phosphoproteome dynamics reveal novel ERK1/2 MAP kinase substrates with broad spectrum of functions. Mol Syst Biol 9, 669 23712012 Curated Info
2	Biarc J, Chalkley RJ, Burlingame AL, Bradshaw RA (2012) The induction of serine/threonine protein phosphorylations by a PDGFR/TrkA chimera in stably transfected PC12 cells. Mol Cell Proteomics 11, 15-30 22027198 Curated Info
3	Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876 22673903 Curated Info
4	Demirkan G, et al. (2011) Phosphoproteomic Profiling of In Vivo Signaling in Liver by the Mammalian Target of Rapamycin Complex 1 (mTORC1). PLoS One 6, e21729 21738781 Curated Info
5	Hoffert JD, et al. (2007) An automated platform for analysis of phosphoproteomic datasets: application to kidney collecting duct phosphoproteins. J Proteome Res 6, 3501-8 17683130 Curated Info
6	Hoffert JD, et al. (2006) Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites. Proc Natl Acad Sci U S A 103, 7159-64 16641100 Curated Info
7	Moser K, White FM (2006) Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS. J Proteome Res 5, 98-104 16396499 Curated Info