Ser325
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Home > Phosphorylation Site Page: > Ser325  -  GJA1 (mouse)

Site Information
NRMGQAGstIsNsHA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449992

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 4 , 5 , 6 , 7 , 9 , 10 ) , mutation of modification site ( 1 , 2 , 3 , 8 ) , phospho-antibody ( 2 , 11 ) , western blotting ( 2 , 8 )
Relevant cell line - cell type - tissue:
'brain, cerebellum' ( 6 ) , 'fat, brown' ( 10 ) , brain ( 3 , 7 , 10 ) , heart ( 2 , 5 , 8 , 9 , 10 , 11 ) , KO ( 11 ) , lung ( 10 ) , MDCK (epithelial) ( 2 ) , muscle ( 1 ) , myocyte-heart ( 1 ) , neuron:postsynaptic density-'brain, hippocampus, CA1 region' ( 4 ) , testis ( 10 )

Upstream Regulation
Treatments:
ischemia ( 8 , 11 ) , long-term_potentiation ( 4 )

Downstream Regulation
Effects of modification on GJA1:
intracellular localization ( 8 , 11 ) , molecular association, regulation ( 1 )
Effects of modification on biological processes:
cytoskeletal reorganization ( 1 ) , endocytosis, inhibited ( 8 ) , signaling pathway regulation ( 2 )
Induce interaction with:
TUBB (human) ( 1 )

References 

1

Himelman E, et al. (2022) A microtubule-connexin-43 regulatory link suppresses arrhythmias and cardiac fibrosis in Duchenne muscular dystrophy mice. Am J Physiol Heart Circ Physiol 323, H983-H995
36206047   Curated Info

2

Solan JL, Marquez-Rosado L, Lampe PD (2019) Cx43 phosphorylation mediated effects on ERK and Akt protect against ischemia reperfusion injury and alter stability of stress-inducible protein NDRG1. J Biol Chem
31189653   Curated Info

3

Freitas-Andrade M, et al. (2019) Targeting MAPK phosphorylation of Connexin43 provides neuroprotection in stroke. J Exp Med 216, 916-935
30872361   Curated Info

4

Li J, et al. (2016) Long-term potentiation modulates synaptic phosphorylation networks and reshapes the structure of the postsynaptic interactome. Sci Signal 9, rs8
27507650   Curated Info

5

Lundby A, et al. (2013) In vivo phosphoproteomics analysis reveals the cardiac targets of β-adrenergic receptor signaling. Sci Signal 6, rs11
23737553   Curated Info

6

Schindler J, Ye J, Jensen ON, Nothwang HG (2013) Monitoring the native phosphorylation state of plasma membrane proteins from a single mouse cerebellum. J Neurosci Methods 213, 153-64
23246975   Curated Info

7

Trinidad JC, et al. (2012) Global identification and characterization of both O-GlcNAcylation and phosphorylation at the murine synapse. Mol Cell Proteomics 11, 215-29
22645316   Curated Info

8

Remo BF, et al. (2011) Phosphatase-resistant gap junctions inhibit pathological remodeling and prevent arrhythmias. Circ Res 108, 1459-66
21527737   Curated Info

9

Deng N, et al. (2011) Phosphoproteome analysis reveals regulatory sites in major pathways of cardiac mitochondria. Mol Cell Proteomics 10, M110.000117
20495213   Curated Info

10

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

11

Lampe PD, Cooper CD, King TJ, Burt JM (2006) Analysis of Connexin43 phosphorylated at S325, S328 and S330 in normoxic and ischemic heart. J Cell Sci 119, 3435-42
16882687   Curated Info

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