Ser311
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser311  -  NFkB-p65 (human)

Site Information
RtyEtFksIMkksPF   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449966

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 2 , 3 ) , mass spectrometry ( 2 , 8 ) , mutation of modification site ( 2 , 3 , 5 , 9 ) , phospho-antibody ( 2 , 3 , 4 , 10 ) , western blotting ( 2 , 3 , 4 )
Disease tissue studied:
colorectal cancer ( 4 ) , colorectal carcinoma ( 4 ) , neuroblastoma ( 2 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 10 ) , 3T3 (fibroblast) ( 5 ) , BAEC (endothelial) ( 9 ) , bEnd3 ( 5 ) , Jurkat (T lymphocyte) ( 8 ) , macrophage-peritoneum ( 3 ) , MEF (fibroblast) ( 3 ) , SKNAS (neural crest) ( 2 ) , SW480 (intestinal) ( 4 )

Upstream Regulation
Putative in vivo kinases:
PKCZ (human) ( 10 )
Kinases, in vitro:
IKKB (human) ( 2 )
Treatments:
glucose_starvation ( 4 ) , TNF ( 10 )

Downstream Regulation
Effects of modification on NFkB-p65:
acetylation ( 6 ) , molecular association, regulation ( 5 )
Effects of modification on biological processes:
transcription, induced ( 3 , 5 )
Induce interaction with:
DNA ( 5 )

References 

1

Shahbazi S, Zakerali T, Frycz B, Kaur J (2020) Impact of novel N-aryl substituted piperamide on NF-kappa B translocation as a potent anti-neuroinflammatory agent. Biomed Pharmacother 127, 110199
32416562   Curated Info

2

Lanucara F, et al. (2016) Dynamic phosphorylation of RelA on Ser42 and Ser45 in response to TNFα stimulation regulates DNA binding and transcription. Open Biol 6
27466442   Curated Info

3

Peng Y, et al. (2016) AGE-RAGE signal generates a specific NF-κB RelA "barcode" that directs collagen I expression. Sci Rep 6, 18822
26729520   Curated Info

4

Ma L, et al. (2013) Control of Nutrient Stress-Induced Metabolic Reprogramming by PKCζ in Tumorigenesis. Cell 152, 599-611
23374352   Curated Info

5

Hochrainer K, Racchumi G, Anrather J (2013) Site-specific phosphorylation of the p65 protein subunit mediates selective gene expression by differential NF-κB and RNA polymerase II promoter recruitment. J Biol Chem 288, 285-93
23100252   Curated Info

6

Clavijo PE, Frauwirth KA (2012) Anergic CD8+ T lymphocytes have impaired NF-κB activation with defects in p65 phosphorylation and acetylation. J Immunol 188, 1213-21
22205033   Curated Info

7

Chang Y, et al. (2011) Structural basis of SETD6-mediated regulation of the NF-kB network via methyl-lysine signaling. Nucleic Acids Res 39, 6380-9
21515635   Curated Info

8

Possemato A (2009) CST Curation Set: 6369; Year: 2009; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pSP
Curated Info

9

Seldon MP, et al. (2007) Heme oxygenase-1 inhibits the expression of adhesion molecules associated with endothelial cell activation via inhibition of NF-kappaB RelA phosphorylation at serine 276. J Immunol 179, 7840-51
18025230   Curated Info

10

Teusch N, Lombardo E, Eddleston J, Knaus UG (2004) The low molecular weight GTPase RhoA and atypical protein kinase Czeta are required for TLR2-mediated gene transcription. J Immunol 173, 507-14
15210811   Curated Info