Tyr775
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Home > Phosphorylation Site Page: > Tyr775  -  PDGFRB (human)

Site Information
SSNyMAPyDNyVPSA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449964

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 4 ) , [32P] bio-synthetic labeling ( 4 ) , immunoprecipitation ( 6 ) , mass spectrometry ( 1 , 2 , 3 ) , mutation of modification site ( 5 , 6 ) , phospho-antibody ( 4 , 5 ) , phosphopeptide mapping ( 4 ) , western blotting ( 6 )
Disease tissue studied:
neuroblastoma ( 1 , 2 , 3 )
Relevant cell line - cell type - tissue:
BT-474 (breast cell) ( 5 ) , COS (fibroblast) ( 5 ) , epithelial ( 6 ) , LAN-6 (neural crest) ( 1 ) , PAE (endothelial) ( 4 , 5 ) , SK-N-BE(2) (neural crest) ( 1 , 2 , 3 )

Upstream Regulation
Putative in vivo kinases:
PDGFRB (human) ( 4 )
Putative upstream phosphatases:
PTPRJ (human) ( 4 )
Treatments:
PDGF ( 4 , 6 )

Downstream Regulation
Effects of modification on PDGFRB:
molecular association, regulation ( 5 )
Induce interaction with:
GRB7 (mouse) ( 5 )

References 

1

Palacios-Moreno J, et al. (2015) Neuroblastoma Tyrosine Kinase Signaling Networks Involve FYN and LYN in Endosomes and Lipid Rafts. PLoS Comput Biol 11, e1004130
25884760   Curated Info

2

Guo A (2010) CST Curation Set: 10551; Year: 2010; Biosample/Treatment: cell line, SKNBE(2)/NGF; Disease: neuroblastoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

3

Guo A (2010) CST Curation Set: 10548; Year: 2010; Biosample/Treatment: cell line, SKNBE(2)/NGF; Disease: neuroblastoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

4

Kovalenko M, et al. (2000) Site-selective dephosphorylation of the platelet-derived growth factor beta-receptor by the receptor-like protein-tyrosine phosphatase DEP-1. J Biol Chem 275, 16219-26
10821867   Curated Info

5

Yokote K, Margolis B, Heldin CH, Claesson-Welsh L (1996) Grb7 is a downstream signaling component of platelet-derived growth factor alpha- and beta-receptors. J Biol Chem 271, 30942-9
8940081   Curated Info

6

Kashishian A, Kazlauskas A, Cooper JA (1992) Phosphorylation sites in the PDGF receptor with different specificities for binding GAP and PI3 kinase in vivo. EMBO J 11, 1373-82
1314164   Curated Info