Ser408
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Home > Phosphorylation Site Page: > Ser408  -  MEF2A (human)

Site Information
SIkSEPIsPPRDRMT   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449949

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 13 ) , mass spectrometry ( 1 , 3 , 4 , 5 , 6 , 8 , 12 ) , mutation of modification site ( 10 , 11 , 13 ) , phospho-antibody ( 10 ) , phosphopeptide mapping ( 13 ) , western blotting ( 10 )
Disease tissue studied:
breast cancer ( 4 ) , breast ductal carcinoma ( 4 ) , HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, triple negative ( 1 , 4 ) , ovarian cancer ( 4 ) , pancreatic ductal adenocarcinoma ( 5 )
Relevant cell line - cell type - tissue:
'neuron, cerebellar granule'-brain ( 10 ) , 'neuron, cortical'-brain ( 13 ) , 'pancreatic, ductal'-pancreas ( 5 ) , 293 (epithelial) ( 13 ) , breast ( 1 , 4 ) , COS (fibroblast) ( 11 , 12 ) , HEK293T (epithelial) ( 10 ) , HeLa (cervical) ( 3 , 8 , 12 ) , Jurkat (T lymphocyte) ( 6 ) , K562 (erythroid) ( 8 ) , neuron-'brain, cerebellum' ( 10 ) , neuron:postsynaptic density-brain ( 10 ) , ovary ( 4 )

Upstream Regulation
Putative in vivo kinases:
CDK5 (human) ( 13 ) , P38A (human) ( 12 )
Kinases, in vitro:
CDK5 (human) ( 13 )
Treatments:
calcineurin ( 10 ) , depolarization ( 10 ) , H2O2 ( 13 ) , nimodipine ( 10 ) , seliciclib ( 13 )

Downstream Regulation
Effects of modification on MEF2A:
sumoylation ( 10 )
Effects of modification on biological processes:
cell differentiation, inhibited ( 10 ) , transcription, altered ( 13 ) , transcription, induced ( 10 , 11 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610
27184836   Curated Info

3

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

4

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

5

Britton D, et al. (2014) Quantification of pancreatic cancer proteome and phosphorylome: indicates molecular events likely contributing to cancer and activity of drug targets. PLoS One 9, e90948
24670416   Curated Info

6

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

7

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

8

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

9

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

10

Shalizi A, et al. (2006) A calcium-regulated MEF2 sumoylation switch controls postsynaptic differentiation. Science 311, 1012-7
16484498   Curated Info

11

Zhu B, Gulick T (2004) Phosphorylation and alternative pre-mRNA splicing converge to regulate myocyte enhancer factor 2C activity. Mol Cell Biol 24, 8264-75
15340086   Curated Info

12

Cox DM, et al. (2003) Phosphorylation motifs regulating the stability and function of myocyte enhancer factor 2A. J Biol Chem 278, 15297-303
12586839   Curated Info

13

Gong X, et al. (2003) Cdk5-mediated inhibition of the protective effects of transcription factor MEF2 in neurotoxicity-induced apoptosis. Neuron 38, 33-46
12691662   Curated Info