Ser293

Site Information
TYRyHGHsMsDPGVs SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 448258

In vivo Characterization
Methods used to characterize site in vivo:	[32P] bio-synthetic labeling ( 22 , 24 ) , back-titration ( 22 , 23 ) , mass spectrometry ( 1 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 ) , phospho-antibody ( 2 , 3 ) , phosphopeptide mapping ( 22 , 23 ) , western blotting ( 2 , 3 )
Disease tissue studied:	type 2 diabetes ( 9 )
Relevant cell line - cell type - tissue:	'brain, brainstem' ( 5 ) , 'brain, cerebellum' ( 5 ) , 'brain, cerebral cortex' ( 1 , 5 ) , 'kidney, inner medulla' ( 19 , 20 ) , adipose tissue ( 3 , 5 ) , brain ( 7 , 8 , 10 , 11 , 12 , 13 , 14 , 16 , 17 , 18 ) , epididymal ( 3 ) , heart ( 5 , 15 , 24 ) , intestine ( 5 ) , kidney ( 5 ) , liver ( 5 , 6 , 9 , 21 ) , lung ( 5 ) , muscle ( 5 ) , neuron-'brain, hippocampus' ( 2 ) , pancreas ( 5 ) , PC-12 (chromaffin) [TrkA (rat), transfection] ( 4 ) , PC-12 (chromaffin) ( 4 ) , spleen ( 5 ) , stomach ( 5 ) , testis ( 5 ) , thymus ( 5 )

Upstream Regulation
Treatments:	bicuculline ( 2 ) , PDGF ( 4 ) , resveratrol ( 3 ) , thiamine ( 1 )

Downstream Regulation
Effects of modification on PDHA1:	acetylation ( 1 ) , enzymatic activity, altered ( 1 )

References
1	Aleshin VA, et al. (2021) Daytime Dependence of the Activity of the Rat Brain Pyruvate Dehydrogenase Corresponds to the Mitochondrial Sirtuin 3 Level and Acetylation of Brain Proteins, All Regulated by Thiamine Administration Decreasing Phosphorylation of PDHA Ser293. Int J Mol Sci 22 34360775 Curated Info
2	Bas-Orth C, Tan YW, Lau D, Bading H (2017) Synaptic Activity Drives a Genomic Program That Promotes a Neuronal Warburg Effect. J Biol Chem 292, 5183-5194 28196867 Curated Info
3	Beaudoin MS, et al. (2013) Resveratrol supplementation improves white adipose tissue function in a depot-specific manner in Zucker diabetic fatty rats. Am J Physiol Regul Integr Comp Physiol 305, R542-51 23824959 Curated Info
4	Biarc J, Chalkley RJ, Burlingame AL, Bradshaw RA (2012) The induction of serine/threonine protein phosphorylations by a PDGFR/TrkA chimera in stably transfected PC12 cells. Mol Cell Proteomics 11, 15-30 22027198 Curated Info
5	Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876 22673903 Curated Info
6	Demirkan G, et al. (2011) Phosphoproteomic Profiling of In Vivo Signaling in Liver by the Mammalian Target of Rapamycin Complex 1 (mTORC1). PLoS One 6, e21729 21738781 Curated Info
7	Hu Y (2010) CST Curation Set: 8877; Year: 2010; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
8	Hu Y (2010) CST Curation Set: 8876; Year: 2010; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
9	Deng WJ, et al. (2010) Proteome, phosphoproteome, and hydroxyproteome of liver mitochondria in diabetic rats at early pathogenic stages. Mol Cell Proteomics 9, 100-16 19700791 Curated Info
10	Guo A (2009) CST Curation Set: 5870; Year: 2009; Biosample/Treatment: tissue, brain/No seizure; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
11	Guo A (2009) CST Curation Set: 5867; Year: 2009; Biosample/Treatment: tissue, brain/seizure; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
12	Guo A (2009) CST Curation Set: 5869; Year: 2009; Biosample/Treatment: tissue, brain/No seizure; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
13	Guo A (2009) CST Curation Set: 5864; Year: 2009; Biosample/Treatment: tissue, brain/seizure; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
14	Guo A (2009) CST Curation Set: 5861; Year: 2009; Biosample/Treatment: tissue, brain/sham; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
15	Feng J, et al. (2008) Phosphoproteome analysis of isoflurane-protected heart mitochondria: phosphorylation of adenine nucleotide translocator-1 on Tyr194 regulates mitochondrial function. Cardiovasc Res 80, 20-9 18558627 Curated Info
16	Guo A (2008) CST Curation Set: 5217; Year: 2008; Biosample/Treatment: tissue, brain/mock surgery; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
17	Guo A (2008) CST Curation Set: 5221; Year: 2008; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
18	Guo A (2008) CST Curation Set: 5222; Year: 2008; Biosample/Treatment: tissue, brain/ischemia; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
19	Hoffert JD, et al. (2007) An automated platform for analysis of phosphoproteomic datasets: application to kidney collecting duct phosphoproteins. J Proteome Res 6, 3501-8 17683130 Curated Info
20	Hoffert JD, et al. (2006) Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites. Proc Natl Acad Sci U S A 103, 7159-64 16641100 Curated Info
21	Moser K, White FM (2006) Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS. J Proteome Res 5, 98-104 16396499 Curated Info
22	Sale GJ, Randle PJ (1982) Occupancy of phosphorylation sites in pyruvate dehydrogenase phosphate complex in rat heart in vivo. Relation to proportion of inactive complex and rate of re-activation by phosphatase. Biochem J 206, 221-9 6293460 Curated Info
23	Sale GJ, Randle PJ (1981) Occupancy of sites of phosphorylation in inactive rat heart pyruvate dehydrogenase phosphate in vivo. Biochem J 193, 935-46 7305968 Curated Info
24	Sale GJ, Randle PJ (1980) Incorporation of [32P]phosphate into the pyruvate dehydrogenase complex in rat heart mitochondria. Biochem J 188, 409-21 7396870 Curated Info