Ser386
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Home > Phosphorylation Site Page: > Ser386  -  RSK2 (mouse)

Site Information
HQLFRGFsFVAITSD   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448065

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 10 ) , mass spectrometry ( 2 , 4 , 5 , 6 , 7 ) , mutation of modification site ( 9 , 10 ) , phospho-antibody ( 8 , 9 , 10 ) , western blotting ( 8 , 9 )
Disease tissue studied:
leukemia ( 6 ) , acute myelogenous leukemia ( 6 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 2 ) , 3T3 (fibroblast) ( 8 ) , 3T3 (fibroblast) [KRas (human), transfection] ( 4 ) , blood ( 6 ) , brain ( 7 ) , COS (fibroblast) ( 9 , 10 ) , dendritic cell ( 8 ) , dendritic cell [MAPKAPK2 (mouse), homozygous knockout] ( 8 ) , dendritic cell [MSK1 (mouse), homozygous knockout] ( 8 ) , embryo ( 7 ) , liver ( 5 , 7 )

Upstream Regulation
Regulatory protein:
MAPKAPK2 (mouse) ( 8 ) , MAPKAPK3 (mouse) ( 8 ) , MEK1 (human) ( 9 )
Putative in vivo kinases:
RSK2 (mouse) ( 9 )
Kinases, in vitro:
ERK1 (mouse) ( 10 ) , ERK2 (human) ( 8 ) , MAPKAPK2 (human) ( 8 ) , PDK1 (human) ( 8 ) , PDK1 (mouse) ( 10 )
Treatments:
anisomycin ( 8 ) , BIRB-0796 ( 8 ) , EGF ( 8 , 9 , 10 ) , LPS ( 8 ) , Pam3CSK4 ( 8 ) , PD184352 ( 8 ) , phorbol_ester ( 8 ) , SB203347 ( 8 ) , SB203580 ( 8 ) , TNF ( 8 )

Downstream Regulation
Effects of modification on RSK2:
enzymatic activity, induced ( 9 , 10 ) , molecular association, regulation ( 9 ) , phosphorylation ( 9 )
Induce interaction with:
PDK1 (human) ( 9 )

References 

1

Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250
27841257   Curated Info

2

Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6
26060331   Curated Info

3

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

4

Gnad F, et al. (2013) Systems-wide Analysis of K-Ras, Cdc42, and PAK4 Signaling by Quantitative Phosphoproteomics. Mol Cell Proteomics 12, 2070-80
23608596   Curated Info

5

Wilson-Grady JT, Haas W, Gygi SP (2013) Quantitative comparison of the fasted and re-fed mouse liver phosphoproteomes using lower pH reductive dimethylation. Methods 61, 277-86
23567750   Curated Info

6

Trost M, et al. (2012) Posttranslational regulation of self-renewal capacity: insights from proteome and phosphoproteome analyses of stem cell leukemia. Blood 120, e17-27
22802335   Curated Info

7

Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096   Curated Info

8

Zaru R, et al. (2007) The MAPK-activated kinase Rsk controls an acute Toll-like receptor signaling response in dendritic cells and is activated through two distinct pathways. Nat Immunol 8, 1227-35
17906627   Curated Info

9

Frödin M, Jensen CJ, Merienne K, Gammeltoft S (2000) A phosphoserine-regulated docking site in the protein kinase RSK2 that recruits and activates PDK1. EMBO J 19, 2924-34
10856237   Curated Info

10

Jensen CJ, et al. (1999) 90-kDa ribosomal S6 kinase is phosphorylated and activated by 3-phosphoinositide-dependent protein kinase-1. J Biol Chem 274, 27168-76
10480933   Curated Info