Thr422
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Home > Phosphorylation Site Page: > Thr422  -  PAK1 (rat)

Site Information
PEQSKRstMVGTPYW   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448177

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 6 ) , immunoprecipitation ( 1 ) , mutation of modification site ( 6 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 ) , western blotting ( 1 , 2 , 3 , 4 , 5 )
Relevant cell line - cell type - tissue:
COS (fibroblast) ( 6 ) , HeLa (cervical) ( 6 ) , hepatic stellate ( 3 ) , L6 (myoblast) [GLUT4 (rat)] ( 5 ) , L6 (myoblast) ( 1 ) , neutrophil-blood ( 2 ) , Schwann ( 4 )

Upstream Regulation
Regulatory protein:
RAC1 (rat) ( 5 )
Putative in vivo kinases:
PAK1 (rat) ( 4 ) , PKACA (rat) ( 4 )
Kinases, in vitro:
PAK1 (rat) ( 6 , 7 )
Treatments:
AG825 ( 4 ) , fMLP ( 2 ) , IL-11 ( 3 ) , insulin ( 1 , 5 ) , IPA-3 ( 1 ) , laminin-1 ( 4 ) , neuregulin ( 4 ) , TCH-116 ( 2 )

Downstream Regulation
Effects of modification on PAK1:
enzymatic activity, induced ( 6 ) , molecular association, regulation ( 1 )
Effects of modification on biological processes:
cytoskeletal reorganization ( 1 )
Induce interaction with:
N-WASP (rat) ( 1 )

References 

1

Tunduguru R, et al. (2017) The actin-related p41ARC subunit contributes to p21-activated kinase-1 (PAK1)-mediated glucose uptake into skeletal muscle cells. J Biol Chem
28972183   Curated Info

2

Tsai YR, et al. (2012) The signaling mechanisms mediating the inhibitory effect of TCH-1116 on formyl peptide-stimulated superoxide anion generation in neutrophils. Eur J Pharmacol 682, 171-80
22510297   Curated Info

3

Zhou L, et al. (2009) Tumor necrosis factor-alpha induced expression of matrix metalloproteinase-9 through p21-activated kinase-1. BMC Immunol 10, 15
19298660   Curated Info

4

Thaxton C, Lopera J, Bott M, Fernandez-Valle C (2008) Neuregulin and laminin stimulate phosphorylation of the NF2 tumor suppressor in Schwann cells by distinct protein kinase A and p21-activated kinase-dependent pathways. Oncogene 27, 2705-15
17998937   Curated Info

5

JeBailey L, et al. (2007) Ceramide- and oxidant-induced insulin resistance involve loss of insulin-dependent Rac-activation and actin remodeling in muscle cells. Diabetes 56, 394-403
17259384   Curated Info

6

Chong C, Tan L, Lim L, Manser E (2001) The mechanism of PAK activation. Autophosphorylation events in both regulatory and kinase domains control activity. J Biol Chem 276, 17347-53
11278486   Curated Info

7

Manser E, et al. (1997) Expression of constitutively active alpha-PAK reveals effects of the kinase on actin and focal complexes. Mol Cell Biol 17, 1129-43
9032240   Curated Info