Thr211
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Home > Phosphorylation Site Page: > Thr211  -  PAK1 (rat)

Site Information
VIEPLPVtPTRDVAT   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448176

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 ) , microscopy-colocalization with upstream kinase ( 5 ) , mutation of modification site ( 4 , 6 ) , phospho-antibody ( 3 , 4 , 5 , 6 ) , western blotting ( 3 , 4 , 6 )
Relevant cell line - cell type - tissue:
'brain, cerebral cortex' ( 5 ) , 'brain, forebrain' ( 2 ) , 'muscle, smooth'-'heart, artery' ( 4 ) , hepatic stellate ( 3 ) , IEC-6 (epithelial) ( 1 ) , neuron-'brain, hippocampus' ( 6 )

Upstream Regulation
Putative in vivo kinases:
CDK5 (mouse) ( 6 ) , ERK2 (rat) ( 4 )
Kinases, in vitro:
ERK2 (rat) ( 4 )
Treatments:
cell_adhesion ( 4 ) , IL-11 ( 3 ) , PD184352 ( 1 ) , PDGF ( 4 ) , serum ( 1 ) , U0126 ( 4 )

Downstream Regulation
Effects of modification on PAK1:
intracellular localization ( 5 )
Effects of modification on biological processes:
cytoskeletal reorganization ( 5 )

References 

1

Courcelles M, et al. (2013) Phosphoproteome dynamics reveal novel ERK1/2 MAP kinase substrates with broad spectrum of functions. Mol Syst Biol 9, 669
23712012   Curated Info

2

Edbauer D, et al. (2009) Identification and characterization of neuronal mitogen-activated protein kinase substrates using a specific phosphomotif antibody. Mol Cell Proteomics 8, 681-95
19054758   Curated Info

3

Zhou L, et al. (2009) Tumor necrosis factor-alpha induced expression of matrix metalloproteinase-9 through p21-activated kinase-1. BMC Immunol 10, 15
19298660   Curated Info

4

Sundberg-Smith LJ, Doherty JT, Mack CP, Taylor JM (2005) Adhesion stimulates direct PAK1/ERK2 association and leads to ERK-dependent PAK1 Thr212 phosphorylation. J Biol Chem 280, 2055-64
15542607   Curated Info

5

Banerjee M, Worth D, Prowse DM, Nikolic M (2002) Pak1 phosphorylation on t212 affects microtubules in cells undergoing mitosis. Curr Biol 12, 1233-9
12176334   Curated Info

6

Rashid T, Banerjee M, Nikolic M (2001) Phosphorylation of Pak1 by the p35/Cdk5 kinase affects neuronal morphology. J Biol Chem 276, 49043-52
11604394   Curated Info