Ser144
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Home > Phosphorylation Site Page: > Ser144  -  PAK1 (rat)

Site Information
SNSQKYMsFTDKsAE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448170

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 6 ) , mass spectrometry ( 2 , 4 , 5 ) , mutation of modification site ( 6 ) , phospho-antibody ( 1 , 3 ) , western blotting ( 1 , 3 )
Relevant cell line - cell type - tissue:
'neuron, cerebellar granule' ( 1 ) , COS (fibroblast) ( 6 ) , HeLa (cervical) ( 6 ) , IEC-6 (epithelial) ( 2 ) , intestine ( 4 ) , kidney ( 4 ) , liver ( 4 , 5 ) , neutrophil-blood ( 3 ) , spleen ( 4 ) , stomach ( 4 )

Upstream Regulation
Kinases, in vitro:
PAK1 (rat) ( 7 )
Treatments:
fMLP ( 3 ) , NSC23766 ( 1 ) , TCH-116 ( 3 )

Downstream Regulation
Effects of modification on PAK1:
enzymatic activity, induced ( 6 )

References 

1

Stankiewicz TR, et al. (2015) Neuronal Apoptosis Induced by Selective Inhibition of Rac GTPase versus Global Suppression of Rho Family GTPases Is Mediated by Alterations in Distinct Mitogen-activated Protein Kinase Signaling Cascades. J Biol Chem 290, 9363-76
25666619   Curated Info

2

Courcelles M, et al. (2013) Phosphoproteome dynamics reveal novel ERK1/2 MAP kinase substrates with broad spectrum of functions. Mol Syst Biol 9, 669
23712012   Curated Info

3

Tsai YR, et al. (2012) The signaling mechanisms mediating the inhibitory effect of TCH-1116 on formyl peptide-stimulated superoxide anion generation in neutrophils. Eur J Pharmacol 682, 171-80
22510297   Curated Info

4

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

5

Moser K, White FM (2006) Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS. J Proteome Res 5, 98-104
16396499   Curated Info

6

Chong C, Tan L, Lim L, Manser E (2001) The mechanism of PAK activation. Autophosphorylation events in both regulatory and kinase domains control activity. J Biol Chem 276, 17347-53
11278486   Curated Info

7

Manser E, et al. (1997) Expression of constitutively active alpha-PAK reveals effects of the kinase on actin and focal complexes. Mol Cell Biol 17, 1129-43
9032240   Curated Info