Thr10
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Home > Phosphorylation Site Page: > Thr10  -  RNF7 (human)

Site Information
DVEDGEEtCALAsHS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449925

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 4 ) , mass spectrometry ( 1 , 3 ) , mutation of modification site ( 2 , 4 ) , phospho-antibody ( 2 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 2 ) , DLD1 (intestinal) ( 2 ) , HeLa (cervical) ( 2 , 4 ) , Jurkat (T lymphocyte) ( 3 )

Upstream Regulation
Putative in vivo kinases:
CK2A1 (human) ( 2 , 4 )
Kinases, in vitro:
CK2A1 (human) ( 4 )
Treatments:
DRB ( 4 ) , heparin sodium ( 4 )

Downstream Regulation
Effects of modification on RNF7:
protein degradation ( 2 )
Effects of modification on biological processes:
cell cycle regulation ( 4 )

References 

1

Gu T (2007) CST Curation Set: 2234; Year: 2007; Biosample/Treatment: cell line, RSK2-1/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

2

He H, et al. (2007) CK2 phosphorylation of SAG at Thr10 regulates SAG stability, but not its E3 ligase activity. Mol Cell Biochem 295, 179-88
16874460   Curated Info

3

Rush J (2005) CST Curation Set: 920; Year: 2005; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (K/R)XX[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) Akt Substrate Antibody Cat#: 9611
Curated Info

4

Kim YS, et al. (2003) Phosphorylation of threonine 10 on CKBBP1/SAG/ROC2/Rbx2 by protein kinase CKII promotes the degradation of IkappaBalpha and p27Kip1. J Biol Chem 278, 28462-9
12748192   Curated Info