Ser535
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Home > Phosphorylation Site Page: > Ser535  -  eIF2B-epsilon (rat)

Site Information
VDPEELDsRAGsPQL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449905

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 7 ) , mass spectrometry ( 2 ) , mutation of modification site ( 4 , 7 ) , peptide sequencing ( 7 ) , phospho-antibody ( 3 , 4 , 5 , 6 , 7 ) , phosphopeptide mapping ( 7 ) , western blotting ( 4 , 5 , 6 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 3 , 5 ) , 293 (epithelial) ( 7 ) , blood ( 2 ) , CHO-K1 (fibroblast) ( 6 ) , intestine ( 2 ) , kidney ( 2 ) , liver ( 2 ) , myocyte-heart ( 4 ) , spleen ( 2 ) , testis ( 2 )

Upstream Regulation
Putative in vivo kinases:
GSK3B (rat) ( 4 )
Kinases, in vitro:
GSK3A (rat) ( 7 ) , GSK3B (human) ( 8 ) , GSK3B (rat) ( 4 )
Treatments:
anti-TNFalpha ( 5 ) , endothelin ( 4 ) , exercise ( 3 ) , insulin ( 6 ) , isoproterenol ( 4 ) , lithium ( 4 , 6 ) , LY294002 ( 6 ) , phorbol_ester ( 6 ) , prazosin ( 4 ) , propranolol ( 4 ) , rapamycin ( 3 ) , SB216763 ( 6 ) , SB415286 ( 6 ) , sepsis ( 5 ) , serum ( 6 )

Downstream Regulation
Effects of modification on biological processes:
cell growth, altered ( 4 ) , cytoskeletal reorganization ( 4 ) , translation, altered ( 4 )

References 

1

Woo CW, Kutzler L, Kimball SR, Tabas I (2012) Toll-like receptor activation suppresses ER stress factor CHOP and translation inhibition through activation of eIF2B. Nat Cell Biol 14, 192-200
22231169   Curated Info

2

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

3

Kubica N, et al. (2005) Resistance exercise increases muscle protein synthesis and translation of eukaryotic initiation factor 2Bepsilon mRNA in a mammalian target of rapamycin-dependent manner. J Biol Chem 280, 7570-80
15591312   Curated Info

4

Hardt SE, Tomita H, Katus HA, Sadoshima J (2004) Phosphorylation of eukaryotic translation initiation factor 2Bepsilon by glycogen synthase kinase-3beta regulates beta-adrenergic cardiac myocyte hypertrophy. Circ Res 94, 926-35
15001529   Curated Info

5

Vary TC, Deiter G, Kimball SR (2002) Phosphorylation of eukaryotic initiation factor eIF2Bepsilon in skeletal muscle during sepsis. Am J Physiol Endocrinol Metab 283, E1032-9
12376332   Curated Info

6

Wang X, et al. (2002) Evidence that the dephosphorylation of Ser(535) in the epsilon-subunit of eukaryotic initiation factor (eIF) 2B is insufficient for the activation of eIF2B by insulin. Biochem J 367, 475-81
12133000   Curated Info

7

Wang X, et al. (2001) Eukaryotic initiation factor 2B: identification of multiple phosphorylation sites in the epsilon-subunit and their functions in vivo. EMBO J 20, 4349-59
11500362   Curated Info

8

Woods YL, et al. (2001) The kinase DYRK phosphorylates protein-synthesis initiation factor eIF2Bepsilon at Ser539 and the microtubule-associated protein tau at Thr212: potential role for DYRK as a glycogen synthase kinase 3-priming kinase. Biochem J 355, 609-15
11311121   Curated Info