Ser307
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Home > Phosphorylation Site Page: > Ser307  -  CAP1 (mouse)

Site Information
SAPKPQtsPsPKPAt   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 485063

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 4 ) , [32P] bio-synthetic labeling ( 4 ) , immunoassay ( 4 ) , immunoprecipitation ( 4 ) , mass spectrometry ( 1 , 3 , 4 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 ) , mutation of modification site ( 4 ) , phospho-antibody ( 4 ) , western blotting ( 4 )
Disease tissue studied:
anthrax infection ( 9 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 3 , 6 ) , 32Dcl3 (myeloid) [FLT3 (mouse), transfection, chimera with human FLT3-ITD mutant (corresponding to wild type P36888 ( 12 ) , 32Dcl3 (myeloid) ( 12 ) , 3T3 (fibroblast) ( 4 ) , brain ( 11 ) , HEK293T (epithelial) ( 4 ) , HeLa (cervical) ( 4 ) , Hepa 1-6 (epithelial) ( 13 ) , hTERT-HPNE (pancreatic) ( 4 ) , liver ( 1 ) , macrophage-bone marrow ( 10 ) , macrophage-bone marrow [DUSP1 (mouse), homozygous knockout] ( 10 ) , macrophage-peritoneum ( 7 ) , MEF (fibroblast) ( 8 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 8 ) , spleen ( 9 )

Upstream Regulation
Treatments:
BIO ( 4 ) , colforsin ( 4 ) , fibronectin ( 4 ) , lithium ( 4 ) , LY294002 ( 4 ) , RKI-1447 ( 4 ) , SB216763 ( 4 ) , siRNA ( 4 ) , U0126 ( 4 )

Downstream Regulation
Effects of modification on CAP1:
intracellular localization ( 4 ) , molecular association, regulation ( 4 )
Effects of modification on biological processes:
cell motility, inhibited ( 4 ) , cytoskeletal reorganization ( 4 )
Induce interaction with:
ACTA1 (human) ( 4 )
Inhibit interaction with:
Cofilin-1 (human) ( 4 )

References 

1

Robles MS, Humphrey SJ, Mann M (2017) Phosphorylation Is a Central Mechanism for Circadian Control of Metabolism and Physiology. Cell Metab 25, 118-127
27818261   Curated Info

2

Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250
27841257   Curated Info

3

Minard AY, et al. (2016) mTORC1 Is a Major Regulatory Node in the FGF21 Signaling Network in Adipocytes. Cell Rep 17, 29-36
27681418   Curated Info

4

Zhou GL, et al. (2014) Phosphorylation of the cytoskeletal protein CAP1 controls its association with cofilin and actin. J Cell Sci 127, 5052-65
25315833   Curated Info

5

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

6

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

7

Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51
22942356   Curated Info

8

Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6
21659605   Curated Info

9

Manes NP, et al. (2011) Discovery of mouse spleen signaling responses to anthrax using label-free quantitative phosphoproteomics via mass spectrometry. Mol Cell Proteomics 10, M110.000927
21189417   Curated Info

10

Weintz G, et al. (2010) The phosphoproteome of toll-like receptor-activated macrophages. Mol Syst Biol 6, 371
20531401   Curated Info

11

Wiƛniewski JR, et al. (2010) Brain phosphoproteome obtained by a FASP-based method reveals plasma membrane protein topology. J Proteome Res 9, 3280-9
20415495   Curated Info

12

Choudhary C, et al. (2009) Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes. Mol Cell 36, 326-39
19854140   Curated Info

13

Pan C, Gnad F, Olsen JV, Mann M (2008) Quantitative phosphoproteome analysis of a mouse liver cell line reveals specificity of phosphatase inhibitors. Proteomics 8, 4534-46
18846507   Curated Info