Thr138
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Home > Phosphorylation Site Page: > Thr138  -  SNAP-25 (human)

Site Information
GGFIRRVtNDARENE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449875

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 1 ) , [32P] bio-synthetic labeling ( 1 ) , mass spectrometry ( 2 ) , mutation of modification site ( 1 , 3 )
Disease tissue studied:
adrenal cancer ( 1 ) , pheochromocytoma ( 1 )
Relevant cell line - cell type - tissue:
chromaffin-adrenal gland ( 3 ) , COS7 (fibroblast) ( 1 ) , Jurkat (T lymphocyte) ( 2 ) , PC-12 (chromaffin) ( 1 )

Upstream Regulation
Kinases, in vitro:
PKACA (human) ( 1 ) , PKCA (human) ( 1 )
Putative upstream phosphatases:
PPP1CA (human) ( 1 ) , PPP2CA (human) ( 1 )
Phosphatases, in vitro:
PPP1CA (human) ( 1 ) , PPP2CA (human) ( 1 )
Treatments:
calyculin_A ( 1 ) , NKH_477 ( 1 ) , okadaic_acid ( 1 ) , phorbol_ester ( 1 )

Downstream Regulation
Effects of modification on SNAP-25:
molecular association, regulation ( 3 )
Effects of modification on biological processes:
exocytosis, induced ( 1 )
Inhibit interaction with:
STX1A (human) ( 3 ) , SYT1 (human) ( 3 )

References 

1

Gao J, et al. (2012) Phospholipase C-related but Catalytically Inactive Protein (PRIP) Modulates Synaptosomal-associated Protein 25 (SNAP-25) Phosphorylation and Exocytosis. J Biol Chem 287, 10565-78
22311984   Curated Info

2

Possemato A (2008) CST Curation Set: 4738; Year: 2008; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (K/R)XX[ST]
Curated Info

3

Yang Y, et al. (2007) Phosphomimetic mutation of Ser-187 of SNAP-25 increases both syntaxin binding and highly Ca2+-sensitive exocytosis. J Gen Physiol 129, 233-44
17325194   Curated Info