Thr138
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Home > Phosphorylation Site Page: > Thr138  -  SNAP-25 (rat)

Site Information
GGFIRRVtNDARENE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449875

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 4 ) , immunoassay ( 1 ) , immunoprecipitation ( 1 , 2 ) , mass spectrometry ( 2 ) , mutation of modification site ( 1 , 2 , 3 , 4 ) , phospho-antibody ( 3 ) , phosphoamino acid analysis ( 4 ) , phosphopeptide mapping ( 4 ) , western blotting ( 1 , 2 )
Disease tissue studied:
adrenal cancer ( 2 , 4 ) , pheochromocytoma ( 2 , 4 )
Relevant cell line - cell type - tissue:
chromaffin-adrenal gland ( 3 ) , HeLa (cervical) ( 4 ) , PC-12 (chromaffin) ( 2 , 4 ) , retinal-retina ( 1 )

Upstream Regulation
Putative in vivo kinases:
PKACA (cow) ( 1 , 3 )
Treatments:
cAMP_analog ( 3 ) , colforsin ( 2 , 3 , 4 ) , IBMX ( 3 ) , KT5720 ( 3 ) , phorbol_ester ( 4 )

Downstream Regulation
Effects of modification on SNAP-25:
molecular association, regulation ( 2 )
Effects of modification on biological processes:
exocytosis, inhibited ( 1 ) , neural plasticity ( 1 ) , signaling pathway regulation ( 1 )
Inhibit interaction with:
STX1A (rat) ( 2 )

References 

1

Hsiao YT, et al. (2019) Presynaptic SNAP-25 regulates retinal waves and retinogeniculate projection via phosphorylation. Proc Natl Acad Sci U S A 116, 3262-3267
30728295   Curated Info

2

Gao J, et al. (2016) Differential role of SNAP-25 phosphorylation by protein kinases A and C in the regulation of SNARE complex formation and exocytosis in PC12 cells. Cell Signal 28, 425-37
26721188   Curated Info

3

Nagy G, et al. (2004) Regulation of releasable vesicle pool sizes by protein kinase A-dependent phosphorylation of SNAP-25. Neuron 41, 417-29
14766180   Curated Info

4

Hepp R, Cabaniols JP, Roche PA (2002) Differential phosphorylation of SNAP-25 in vivo by protein kinase C and protein kinase A. FEBS Lett 532, 52-6
12459461   Curated Info