Ser256
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Home > Phosphorylation Site Page: > Ser256  -  AQP2 (mouse)

Site Information
REVRRRQsVELHsPQ   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449854

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 2 ) , mass spectrometry ( 8 , 10 ) , mutation of modification site ( 3 , 4 , 5 , 11 , 13 , 14 , 16 ) , phospho-antibody ( 1 , 2 , 3 , 6 , 7 , 9 , 12 , 13 , 14 , 16 ) , western blotting ( 1 , 2 , 3 , 5 , 6 , 7 , 9 , 11 , 12 , 13 )
Disease tissue studied:
kidney cancer ( 12 ) , congenital progressive hydronephrosis ( 16 )
Relevant cell line - cell type - tissue:
'kidney, inner medulla' ( 7 ) , 'kidney, tubule' ( 1 ) , HEK293T (epithelial) ( 4 ) , kidney ( 8 , 12 , 14 , 16 ) , LLC-PK1 (renal) ( 3 ) , MDCK (epithelial) ( 5 , 6 , 11 , 14 ) , mpkCCD (renal) ( 9 , 10 ) , oocyte ( 13 )

Upstream Regulation
Regulatory protein:
ADCY6 (mouse) ( 7 )
Treatments:
desmopressin ( 2 , 7 ) , EGF ( 3 ) , erlotinib ( 3 ) , NH4Cl ( 12 ) , vasopressin ( 3 )

Downstream Regulation
Effects of modification on AQP2:
activity, induced ( 13 ) , intracellular localization ( 5 , 13 , 16 ) , molecular association, regulation ( 4 , 11 ) , phosphorylation ( 13 , 14 ) , protein stabilization ( 11 ) , receptor internalization, inhibited ( 5 )
Effects of modification on biological processes:
endocytosis, altered ( 11 ) , exocytosis, altered ( 11 )
Induce interaction with:
14-3-3 theta (human) ( 4 ) , 14-3-3 zeta (human) ( 4 )
Inhibit interaction with:
ACTG1 (human) ( 11 ) , ANXA2 (dog) ( 11 ) , HSC70 (human) ( 11 )

Disease / Diagnostics Relevance
Relevant diseases:
congenital progressive hydronephrosis ( 16 )

References 

1

Fenton RA, et al. (2017) Renal tubular NHE3 is required in the maintenance of water and sodium chloride homeostasis. Kidney Int 92, 397-414
28385297   Curated Info

2

Yui N, Sasaki S, Uchida S (2017) Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochem Biophys Res Commun 482, 524-529
27889609   Curated Info

3

Cheung PW, et al. (2016) EGF Receptor Inhibition by Erlotinib Increases Aquaporin 2-Mediated Renal Water Reabsorption. J Am Soc Nephrol 27, 3105-3116
27694161   Curated Info

4

Moeller HB, et al. (2016) Regulation of the Water Channel Aquaporin-2 via 14-3-3θ and -ζ. J Biol Chem 291, 2469-84
26645691   Curated Info

5

Moeller HB, et al. (2014) Phosphorylation and ubiquitylation are opposing processes that regulate endocytosis of the water channel aquaporin-2. J Cell Sci 127, 3174-83
24876223   Curated Info

6

Feinstein TN, et al. (2013) Noncanonical control of vasopressin receptor type 2 signaling by retromer and arrestin. J Biol Chem 288, 27849-60
23935101   Curated Info

7

Rieg T, et al. (2010) Adenylate cyclase 6 determines cAMP formation and aquaporin-2 phosphorylation and trafficking in inner medulla. J Am Soc Nephrol 21, 2059-68
20864687   Curated Info

8

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

9

Xie L, et al. (2010) Quantitative analysis of aquaporin-2 phosphorylation. Am J Physiol Renal Physiol 298, F1018-23
20089674   Curated Info

10

Rinschen MM, et al. (2010) Quantitative phosphoproteomic analysis reveals vasopressin V2-receptor-dependent signaling pathways in renal collecting duct cells. Proc Natl Acad Sci U S A 107, 3882-7
20139300   Curated Info

11

Moeller HB, Praetorius J, Rützler MR, Fenton RA (2010) Phosphorylation of aquaporin-2 regulates its endocytosis and protein-protein interactions. Proc Natl Acad Sci U S A 107, 424-9
19966308   Curated Info

12

Nowik M, et al. (2010) Induction of metabolic acidosis with ammonium chloride (NH4Cl) in mice and rats--species differences and technical considerations. Cell Physiol Biochem 26, 1059-72
21220937   Curated Info

13

Moeller HB, Macaulay N, Knepper MA, Fenton RA (2009) Role of multiple phosphorylation sites in the COOH-terminal tail of aquaporin-2 for water transport: evidence against channel gating. Am J Physiol Renal Physiol 296, F649-57
19144687   Curated Info

14

Hoffert JD, et al. (2008) Vasopressin-stimulated increase in phosphorylation at Ser269 potentiates plasma membrane retention of aquaporin-2. J Biol Chem 283, 24617-27
18606813   Curated Info

15

Shi PP, et al. (2007) Nephrogenic diabetes insipidus in mice caused by deleting COOH-terminal tail of aquaporin-2. Am J Physiol Renal Physiol 292, F1334-44
17229678   Curated Info

16

McDill BW, et al. (2006) Congenital progressive hydronephrosis (cph) is caused by an S256L mutation in aquaporin-2 that affects its phosphorylation and apical membrane accumulation. Proc Natl Acad Sci U S A 103, 6952-7
16641094   Curated Info

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