Ser469
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.6.0.4
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser469  -  PFKFB2 (rat)

Site Information
PVRMRRNsFtPLSSS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448460

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 3 ) , mass spectrometry ( 1 , 2 ) , mutation of modification site ( 3 ) , phospho-antibody ( 3 ) , western blotting ( 3 )
Disease tissue studied:
adrenal cancer ( 2 ) , pheochromocytoma ( 2 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 3 ) , heart ( 3 ) , intestine ( 1 ) , lung ( 1 ) , PC-12 (chromaffin) ( 2 ) , stomach ( 1 ) , testis ( 1 )

Upstream Regulation
Putative in vivo kinases:
AMPKA1 (rat) ( 3 )
Kinases, in vitro:
AMPKA1 (rat) ( 3 )
Treatments:
oligomycin ( 3 )

Downstream Regulation
Effects of modification on PFKFB2:
enzymatic activity, induced ( 3 )

References 

1

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

2

Zhou J (2009) CST Curation Set: 6822; Year: 2009; Biosample/Treatment: cell line, PC-12/untreated; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

3

Marsin AS, et al. (2000) Phosphorylation and activation of heart PFK-2 by AMPK has a role in the stimulation of glycolysis during ischaemia. Curr Biol 10, 1247-55
11069105   Curated Info

4

Deprez J, et al. (2000) Partial purification and characterization of a wortmannin-sensitive and insulin-stimulated protein kinase that activates heart 6-phosphofructo-2-kinase. Biochem J 347 Pt 1, 305-12
10727432   Curated Info