Ser256

Site Information
VYVSRRRsRsPLDKD GenPept Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 468598

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 4 , 5 )
Disease tissue studied:	adrenal cancer ( 4 , 5 ) , pheochromocytoma ( 4 , 5 )
Relevant cell line - cell type - tissue:	'brain, cerebellum' ( 2 ) , heart ( 2 ) , IEC-6 (epithelial) ( 1 ) , liver ( 3 ) , lung ( 2 ) , PC-12 (chromaffin) ( 4 , 5 ) , spleen ( 2 ) , stomach ( 2 ) , thymus ( 2 )

Upstream Regulation
Treatments:	serum ( 1 )

References
1	Courcelles M, et al. (2013) Phosphoproteome dynamics reveal novel ERK1/2 MAP kinase substrates with broad spectrum of functions. Mol Syst Biol 9, 669 23712012 Curated Info
2	Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876 22673903 Curated Info
3	Demirkan G, et al. (2011) Phosphoproteomic Profiling of In Vivo Signaling in Liver by the Mammalian Target of Rapamycin Complex 1 (mTORC1). PLoS One 6, e21729 21738781 Curated Info
4	Zhou J (2009) CST Curation Set: 6823; Year: 2009; Biosample/Treatment: cell line, PC-12/forskolin; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS/T) Immunoaffinity Beads Cat#: 1984 Curated Info
5	Zhou J (2009) CST Curation Set: 6822; Year: 2009; Biosample/Treatment: cell line, PC-12/untreated; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS/T) Immunoaffinity Beads Cat#: 1984 Curated Info