Ser244
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Home > Phosphorylation Site Page: > Ser244  -  PDK1 (rat)

Site Information
SKQARANsFVGTAQY   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447693

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 2 , 4 , 5 , 7 ) , phospho-antibody ( 3 , 6 , 8 ) , western blotting ( 3 , 6 , 8 )
Relevant cell line - cell type - tissue:
'brain, brainstem' ( 5 ) , 'brain, cerebellum' ( 5 ) , 'brain, cerebral cortex' ( 5 ) , adipose tissue ( 5 ) , blood ( 5 ) , heart ( 5 , 8 ) , IEC-6 (epithelial) ( 2 ) , intestine ( 5 ) , kidney ( 5 ) , liver ( 5 , 7 ) , lung ( 5 ) , muscle ( 5 ) , neutrophil-blood ( 3 ) , pancreas ( 5 ) , PC-12 (chromaffin) [TrkA (rat), transfection] ( 4 ) , PC-12 (chromaffin) ( 4 ) , spleen ( 5 ) , stomach ( 5 ) , testis ( 5 ) , thymus ( 5 ) , VSMC ( 6 )

Upstream Regulation
Regulatory protein:
PKCA (rat) ( 6 )
Treatments:
fMLP ( 3 ) , TCH-116 ( 3 )

Downstream Regulation
Effects of modification on PDK1:
enzymatic activity, induced ( 9 )

References 

1

Lin G, Brownsey RW, Macleod KM (2014) Complex regulation of PKCĪ²2 and PDK-1/AKT by ROCK2 in diabetic heart. PLoS One 9, e86520
24466133   Curated Info

2

Courcelles M, et al. (2013) Phosphoproteome dynamics reveal novel ERK1/2 MAP kinase substrates with broad spectrum of functions. Mol Syst Biol 9, 669
23712012   Curated Info

3

Tsai YR, et al. (2012) The signaling mechanisms mediating the inhibitory effect of TCH-1116 on formyl peptide-stimulated superoxide anion generation in neutrophils. Eur J Pharmacol 682, 171-80
22510297   Curated Info

4

Biarc J, Chalkley RJ, Burlingame AL, Bradshaw RA (2012) The induction of serine/threonine protein phosphorylations by a PDGFR/TrkA chimera in stably transfected PC12 cells. Mol Cell Proteomics 11, 15-30
22027198   Curated Info

5

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

6

Nazarewicz RR, et al. (2011) Early endosomal antigen 1 (EEA1) is an obligate scaffold for angiotensin II-induced, PKC-alpha-dependent Akt activation in endosomes. J Biol Chem 286, 2886-95
21097843   Curated Info

7

Demirkan G, et al. (2011) Phosphoproteomic Profiling of In Vivo Signaling in Liver by the Mammalian Target of Rapamycin Complex 1 (mTORC1). PLoS One 6, e21729
21738781   Curated Info

8

Shinmura K, Nagai M, Tamaki K, Bolli R (2008) Loss of ischaemic preconditioning in ovariectomized rat hearts: possible involvement of impaired protein kinase C epsilon phosphorylation. Cardiovasc Res 79, 387-94
18390563   Curated Info

9

Chen H, et al. (2001) Insulin stimulates increased catalytic activity of phosphoinositide-dependent kinase-1 by a phosphorylation-dependent mechanism. Biochemistry 40, 11851-9
11570885   Curated Info