Ser328
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Home > Phosphorylation Site Page: > Ser328  -  RAD9A (human)

Site Information
VLPsIsLsPGPQPPK   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449812
Available spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 25 , 26 ) , flow cytometry ( 10 ) , immunoprecipitation ( 12 , 18 ) , mass spectrometry ( 1 , 3 , 4 , 5 , 6 , 7 , 9 , 13 , 15 , 16 , 17 , 20 , 21 , 22 , 23 , 24 , 26 ) , mutation of modification site ( 2 , 10 , 12 , 14 , 18 , 19 , 25 , 26 ) , peptide sequencing ( 26 ) , phospho-antibody ( 2 , 10 , 12 , 19 ) , western blotting ( 2 , 10 , 12 , 18 , 19 )
Disease tissue studied:
breast cancer ( 5 ) , breast ductal carcinoma ( 5 ) , HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, surrounding tissue ( 1 ) , breast cancer, triple negative ( 1 ) , leukemia ( 16 , 17 , 20 , 21 , 24 ) , acute myelogenous leukemia ( 16 , 17 , 24 ) , chronic myelogenous leukemia ( 20 , 21 ) , lung cancer ( 3 , 6 ) , non-small cell lung cancer ( 6 ) , non-small cell lung adenocarcinoma ( 3 , 6 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
TLK1 (human) ( 2 )
Putative in vivo kinases:
CDK1 (human) ( 25 ) , CDK2 (human) ( 12 ) , TLK1 (human) ( 18 )
Kinases, in vitro:
CDK1 (human) ( 25 ) , CDK2 (human) ( 12 ) , TLK1 (human) ( 10 , 18 )
Treatments:
adriamycin ( 2 ) , dasatinib ( 15 ) , etoposide ( 12 ) , ionizing_radiation ( 19 ) , ischemia ( 5 )

Downstream Regulation
Effects of modification on RAD9A:
intracellular localization ( 2 , 12 ) , molecular association, regulation ( 18 )
Effects of modification on biological processes:
apoptosis, induced ( 12 ) , cell cycle regulation ( 2 , 14 , 25 ) , chromatin organization, altered ( 18 )
Induce interaction with:
TLK1 (human) ( 18 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Awate S, De Benedetti A (2016) TLK1B mediated phosphorylation of Rad9 regulates its nuclear/cytoplasmic localization and cell cycle checkpoint. BMC Mol Biol 17, 3
26860083   Curated Info

3

Tsai CF, et al. (2015) Large-scale determination of absolute phosphorylation stoichiometries in human cells by motif-targeting quantitative proteomics. Nat Commun 6, 6622
25814448   Curated Info

4

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

5

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

6

Rikova K, Hall B (2013) CST Curation Set: 20738, 21165, 30164, 30165, 30166; Year: 2013; Biosample/Treatment: cell line, H1650, HCC827, H1975, Calu-3, H2106; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

7

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

8

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

9

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

10

Kelly R, Davey SK (2013) Tousled-like kinase-dependent phosphorylation of rad9 plays a role in cell cycle progression and g2/m checkpoint exit. PLoS One 8, e85859
24376897   Curated Info

11

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

12

Zhan Z, et al. (2012) Phosphorylation of Rad9 at Serine 328 by Cyclin A-Cdk2 Triggers Apoptosis via Interfering Bcl-xL. PLoS One 7, e44923
23028682   Curated Info

13

Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53
20363803   Curated Info

14

Greer Card DA, Sierant ML, Davey S (2010) Rad9A is required for G2 decatenation checkpoint and to prevent endoreduplication in response to topoisomerase II inhibition. J Biol Chem 285, 15653-61
20305300   Curated Info

15

Pan C, Olsen JV, Daub H, Mann M (2009) Global effects of kinase inhibitors on signaling networks revealed by quantitative phosphoproteomics. Mol Cell Proteomics 8, 2796-808
19651622   Curated Info

16

Gu T (2009) CST Curation Set: 7660; Year: 2009; Biosample/Treatment: tissue, bone marrow/untreated; Disease: acute myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: PXpSP, pSPX(K/R) Antibodies Used to Purify Peptides prior to LCMS: Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb Cat#: 2325, PTMScan(R) Phospho-MAPK/CDK Substrate Motif (PXS*P, S*PXK/R) Immunoaffinity Beads Cat#: 1982
Curated Info

17

Gu T (2009) CST Curation Set: 7659; Year: 2009; Biosample/Treatment: tissue, bone marrow/untreated; Disease: acute myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: PXpSP, pSPX(K/R) Antibodies Used to Purify Peptides prior to LCMS: Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb Cat#: 2325, PTMScan(R) Phospho-MAPK/CDK Substrate Motif (PXS*P, S*PXK/R) Immunoaffinity Beads Cat#: 1982
Curated Info

18

Sunavala-Dossabhoy G, De Benedetti A (2009) Tousled homolog, TLK1, binds and phosphorylates Rad9; TLK1 acts as a molecular chaperone in DNA repair. DNA Repair (Amst) 8, 87-102
18940270   Curated Info

19

Canfield C, Rains J, De Benedetti A (2009) TLK1B promotes repair of DSBs via its interaction with Rad9 and Asf1. BMC Mol Biol 10, 110
20021694   Curated Info

20

Stokes M (2008) CST Curation Set: 4392; Year: 2008; Biosample/Treatment: cell line, K562/untreated; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

21

Stokes M (2008) CST Curation Set: 4394; Year: 2008; Biosample/Treatment: cell line, K562/untreated; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

22

Possemato A (2008) CST Curation Set: 4342; Year: 2009; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: PXpSP, pSPX(K/R) Antibodies Used to Purify Peptides prior to LCMS: Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb Cat#: 2325, PTMScan(R) Phospho-MAPK/CDK Substrate Motif (PXS*P, S*PXK/R) Immunoaffinity Beads Cat#: 1982
Curated Info

23

Stokes M (2008) CST Curation Set: 3885; Year: 2008; Biosample/Treatment: cell line, Jurkat/pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

24

Gu T (2007) CST Curation Set: 2593; Year: 2007; Biosample/Treatment: tissue, bone marrow/-; Disease: acute myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: PXpSP, pSPX(K/R) Antibodies Used to Purify Peptides prior to LCMS: Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb Cat#: 2325, PTMScan(R) Phospho-MAPK/CDK Substrate Motif (PXS*P, S*PXK/R) Immunoaffinity Beads Cat#: 1982
Curated Info

25

St Onge RP, Besley BD, Pelley JL, Davey S (2003) A role for the phosphorylation of hRad9 in checkpoint signaling. J Biol Chem 278, 26620-8
12734188   Curated Info

26

Roos-Mattjus P, et al. (2003) Phosphorylation of human Rad9 is required for genotoxin-activated checkpoint signaling. J Biol Chem 278, 24428-37
12709442   Curated Info