Ser194
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Home > Phosphorylation Site Page: > Ser194  -  RALA (human)

Site Information
NGKKKRKsLAKRIRE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 483989

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 3 ) , mass spectrometry ( 4 , 5 ) , microscopy-colocalization with upstream kinase ( 3 ) , mutation of modification site ( 1 , 2 , 3 , 5 ) , phospho-antibody ( 5 ) , western blotting ( 3 , 5 )
Disease tissue studied:
breast cancer ( 4 ) , breast ductal carcinoma ( 4 ) , lung cancer ( 5 ) , non-small cell lung cancer ( 5 ) , non-small cell lung adenocarcinoma ( 5 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
AurA (human) ( 3 )
Putative upstream phosphatases:
PPP2CA (human) ( 5 )

Downstream Regulation
Effects of modification on RALA:
enzymatic activity, induced ( 5 ) , intracellular localization ( 3 ) , molecular association, regulation ( 3 )
Effects of modification on biological processes:
carcinogenesis, induced ( 5 ) , cell growth, altered ( 3 ) , cell growth, induced ( 5 ) , cytoskeletal reorganization ( 3 )

Disease / Diagnostics Relevance
Relevant diseases:
pancreatic cancer ( 3 )

References 

1

Pollock SR, et al. (2019) RalA and RalB relocalization to depolarized mitochondria depends on clathrin-mediated endocytosis and facilitates TBK1 activation. PLoS One 14, e0214764
30995277   Curated Info

2

Kashatus DF, et al. (2011) RALA and RALBP1 regulate mitochondrial fission at mitosis. Nat Cell Biol 13, 1108-15
21822277   Curated Info

3

Lim KH, et al. (2010) Aurora-A phosphorylates, activates, and relocalizes the small GTPase RalA. Mol Cell Biol 30, 508-23
19901077   Curated Info

4

Possemato A (2008) CST Curation Set: 5239; Year: 2008; Biosample/Treatment: cell line, MCF-7/serum starved; Disease: breast adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

5

Sablina AA, et al. (2007) The tumor suppressor PP2A Abeta regulates the RalA GTPase. Cell 129, 969-82
17540176   Curated Info