Ser132

Site Information
ssLRRHGsMVsLVSG SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 483985

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 2 , 3 , 4 , 5 )
Relevant cell line - cell type - tissue:	'3T3-L1, differentiated' (adipocyte) ( 3 ) , 'fat, brown' ( 5 ) , 3T3 (fibroblast) [CDC42 (human), transfection] ( 2 ) , 3T3 (fibroblast) [KRas (human), transfection] ( 2 ) , 3T3 (fibroblast) ( 2 ) , brain ( 5 ) , heart ( 5 ) , kidney ( 5 ) , liver ( 5 ) , lung ( 5 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 4 ) , pancreas ( 5 ) , spleen ( 5 )

Upstream Regulation
Regulatory protein:	KRas (mouse) ( 2 )
Treatments:	insulin ( 3 ) , LY294002 ( 3 ) , MK-2206 ( 3 )

References
1	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
2	Gnad F, et al. (2013) Systems-wide Analysis of K-Ras, Cdc42, and PAK4 Signaling by Quantitative Phosphoproteomics. Mol Cell Proteomics 12, 2070-80 23608596 Curated Info
3	Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20 23684622 Curated Info
4	Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6 21659605 Curated Info
5	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info