Ser637

Site Information
VPVARKLsAREQRDC SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 483971

In vivo Characterization
Methods used to characterize site in vivo:	immunoassay ( 1 , 17 , 34 ) , immunoprecipitation ( 25 , 41 , 45 , 54 ) , mass spectrometry ( 44 , 45 ) , modification-specific antibody ( 25 ) , mutation of modification site ( 17 , 21 , 25 , 34 , 41 , 42 , 44 , 47 , 51 , 52 , 53 , 54 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 33 , 34 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 53 , 54 ) , western blotting ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 33 , 34 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 53 )
Disease tissue studied:	adrenal cancer ( 45 ) , pheochromocytoma ( 45 ) , bone cancer ( 3 ) , brain cancer ( 7 ) , glioblastoma ( 7 ) , glioma ( 7 ) , breast cancer ( 13 , 15 , 30 , 36 , 38 , 40 ) , colorectal cancer ( 6 ) , colorectal carcinoma ( 6 , 20 ) , HNSCC ( 5 ) , hypopharyngeal cancer ( 34 ) , leukemia ( 44 ) , acute myelogenous leukemia ( 44 ) , acute erythroid leukemias, including erythroleukemia (M6a) and very rare pure erythroid leukemia (M6b) ( 44 ) , liver cancer ( 8 , 18 , 42 ) , hepatocellular carcinoma ( 18 , 42 ) , lung cancer ( 36 ) , non-small cell lung cancer ( 36 ) , non-small cell lung adenocarcinoma ( 36 ) , non-small cell large cell lung carcinoma ( 36 ) , nasopharyngeal carcinoma ( 34 ) , neuroblastoma ( 45 , 51 ) , OSCC ( 5 ) , ovarian cancer ( 11 ) , pancreatic cancer ( 50 ) , pancreatic carcinoma ( 50 ) , multiple myeloma ( 12 ) , prostate cancer ( 36 ) , melanoma skin cancer ( 12 ) , DKD ( 9 )
Relevant cell line - cell type - tissue:	'muscle, smooth'-pulmonary artery ( 2 ) , 'stem, mesenchymal' ( 43 ) , 293 (epithelial) ( 14 , 27 ) , 501mel ( 12 ) , 95-C ( 16 ) , 95-D ( 16 ) , A2780 (ovarian) ( 11 ) , A375 (melanocyte) ( 12 ) , A549 (pulmonary) ( 36 ) , BaF3 ('B lymphocyte, precursor') ( 44 ) , BEL7402 (hepatic) ( 42 ) , beta-pancreas ( 50 ) , BJ (fibroblast) ( 25 ) , brain ( 17 , 48 ) , CAL33 ( 5 ) , CNE1-LMP1 ( 34 ) , CNE1 ( 34 ) , COC1/DDP ( 11 ) , DU 145 (prostate cell) ( 36 ) , EA.hy 926 (endothelial) ( 31 ) , endothelial-aorta ( 22 ) , fibroblast-foreskin ( 30 ) , glial-brain ( 24 ) , H9c2 (myoblast) ( 26 , 33 , 41 ) , HaCaT (keratinocyte) ( 10 ) , HCT15 (intestinal) ( 6 ) , HEK293T (epithelial) ( 25 , 41 ) , HeLa (cervical) ( 29 , 46 , 51 , 53 , 54 ) , HepG2 (hepatic) ( 8 ) , HFF1 (fibroblast) ( 14 ) , HK-2 (renal tubule cell) ( 9 ) , HK2 (epithelial) ( 21 , 39 ) , HLF (hepatic) ( 42 ) , HN6 (endothelial) ( 5 ) , HNE-2 ( 34 ) , HONE-1 ( 34 ) , HT-22 (neuron) ( 4 , 23 ) , Huh7 (hepatic) ( 18 ) , INS-1 (pancreatic) ( 50 ) , keratinocyte ( 10 ) , kidney ( 9 ) , lung ( 36 ) , MCF-7 (breast cell) ( 15 , 30 , 36 , 38 , 40 ) , MDA-MB-231 (breast cell) ( 13 , 40 ) , MEF (fibroblast) ( 36 , 52 ) , Molm 14 (myeloid) ( 44 ) , MV4-11 (macrophage) ( 44 ) , myocyte-heart ( 41 ) , NCI-H1299 (pulmonary) ( 36 ) , NCI-H460 (pulmonary) ( 36 ) , neuron-'brain, cerebral cortex' ( 49 ) , neutrophil ( 28 ) , NRK49F (fibroblast) ( 37 ) , PC-12 (chromaffin) ( 45 ) , PT130 ( 20 ) , RAW 264.7 (macrophage) ( 19 ) , SH-SY5Y (neural crest) ( 45 , 51 ) , SKNSH (neural crest) ( 45 ) , SNU-449 (hepatic) ( 18 ) , spinal cord ( 1 ) , stem ( 24 ) , SUM159 (breast cell) ( 36 ) , T-ALL (T lymphocyte) ( 43 ) , T47D (breast cell) ( 38 ) , TF-1 (erythroid) ( 44 ) , U-251 MG (glial) ( 7 ) , U2OS (bone cell) ( 3 ) , U87MG (glial) ( 7 ) , ZR-75-1 (breast cell) ( 15 )

Upstream Regulation
Regulatory protein:	AMPKA1 (human) ( 30 ) , ASNS (human) ( 16 ) , BLOC1S1 (human) ( 8 ) , caveolin-1 (human) ( 13 ) , CDK5 (human) ( 40 ) , CNR1 (human) ( 39 ) , CPT1A (human) ( 24 ) , CUL4A (human) ( 11 ) , DAP3 (human) ( 46 ) , DDB1 (human) ( 11 ) , HMGB1 (human) ( 31 ) , HSP90A (human) ( 17 ) , KIF1B (mouse) ( 45 ) , LIMK2 (human) ( 48 ) , MEK1 (human) ( 25 ) , PIEZO1 (human) ( 1 ) , RAB20 (human) ( 23 ) , RAB32 (human) ( 7 ) , RAP2C (human) ( 12 ) , ROCK1 (human) ( 47 ) , SIRT1 (human) ( 30 ) , SIRT3 (human) ( 21 ) , STMP1 (human) ( 18 ) , TNFAIP8L3 (human) ( 5 )
Putative in vivo kinases:	AMPKA1 (human) ( 21 , 34 ) , Pim1 (human) ( 36 ) , PKACA (human) ( 13 , 29 , 30 , 39 , 42 )
Kinases, in vitro:	PKACA (human) ( 54 ) , PRKD1 (human) ( 41 )
Putative upstream phosphatases:	PGAM5 (human) ( 5 , 9 , 14 ) , PPP3CA (human) ( 30 )
Treatments:	acadesine ( 30 , 50 ) , ACEA ( 39 ) , adriamycin ( 33 ) , anandamide ( 39 ) , antimycin_A ( 29 ) , arachidonic_acid ( 53 ) , AZD1208 ( 28 , 36 ) , butaprost ( 2 ) , cAMP_analog ( 30 , 44 ) , CCCP ( 29 ) , celastrol ( 19 ) , ciclosporin ( 40 ) , cisplatin ( 9 , 11 , 34 ) , CoCl2 ( 6 ) , coculture ( 43 ) , colforsin ( 54 ) , compound_C ( 30 , 33 ) , crenolanib ( 44 ) , Cucurbitacin-E ( 34 ) , docetaxel ( 36 ) , doxycycline ( 48 ) , Dynasore ( 29 ) , Erastin ( 17 ) , erucin ( 47 ) , FCCP ( 50 ) , FK506 ( 4 , 30 ) , FK866 ( 30 ) , flavopiridol ( 48 ) , fungal infection ( 14 ) , GSK621 ( 11 ) , H-89 ( 2 , 13 , 29 , 39 , 44 ) , hypothermia ( 22 ) , hypoxia ( 27 ) , IBMX ( 30 , 54 ) , IL-27 ( 10 ) , ionomycin ( 3 ) , L-glutamine_withdrawal ( 15 ) , light ( 3 ) , lncRNAs ( 12 , 26 ) , Mdivi-1 ( 24 , 27 , 29 ) , metformin ( 34 ) , MRE-269 ( 2 ) , NGF_withdrawal ( 45 ) , nicotinamide ( 30 ) , NKH_477 ( 29 , 30 , 39 ) , nocodazole ( 29 ) , OGD/R ( 23 ) , oleic_acid ( 39 ) , palmitate ( 20 , 39 , 50 ) , PD0325901 ( 27 ) , PF04418948 ( 2 ) , PIM447 ( 36 ) , pirinixic acid ( 48 ) , RO1138452 ( 2 ) , rotenone ( 29 ) , sanglifehrin A ( 40 ) , selisistat ( 30 ) , sevoflurane ( 4 ) , Shenmai ( 33 ) , siRNA ( 5 , 14 , 21 , 30 ) , sirtinol ( 30 ) , SRT1720 ( 30 ) , status epilepticus ( 48 ) , stiff ECM substrate ( 1 ) , tamoxifen ( 15 , 38 ) , TGF-beta ( 37 ) , Treprostinil ( 2 ) , unilateral ureteral obstruction ( 9 ) , Wnt3a ( 16 ) , Y27632 ( 47 , 48 )

Downstream Regulation
Effects of modification on DRP1:	activity, induced ( 38 ) , activity, inhibited ( 13 , 24 , 28 , 30 , 36 , 44 ) , enzymatic activity, inhibited ( 54 ) , intracellular localization ( 14 , 21 , 22 , 23 , 29 , 30 , 34 , 36 , 39 , 42 , 47 , 52 , 53 ) , molecular association, regulation ( 54 ) , protein conformation ( 54 )
Effects of modification on biological processes:	apoptosis, inhibited ( 21 , 26 , 34 , 36 , 47 ) , autophagy, induced ( 29 ) , autophagy, inhibited ( 30 ) , carcinogenesis, induced ( 5 , 16 , 17 , 34 , 36 ) , carcinogenesis, inhibited ( 24 ) , cell cycle regulation ( 29 ) , cell differentiation, induced ( 24 ) , cell growth, induced ( 16 , 17 ) , cell growth, inhibited ( 2 , 14 ) , cell motility, induced ( 13 ) , cytoskeletal reorganization ( 13 ) , signaling pathway regulation ( 29 )

Downstream Regulation

Effects of modification on DRP1:

activity, induced ( 38 ) , activity, inhibited ( 13 , 24 , 28 , 30 , 36 , 44 ) , enzymatic activity, inhibited ( 54 ) , intracellular localization ( 14 , 21 , 22 , 23 , 29 , 30 , 34 , 36 , 39 , 42 , 47 , 52 , 53 ) , molecular association, regulation ( 54 ) , protein conformation ( 54 )

Effects of modification on biological processes:

apoptosis, inhibited ( 21 , 26 , 34 , 36 , 47 ) , autophagy, induced ( 29 ) , autophagy, inhibited ( 30 ) , carcinogenesis, induced ( 5 , 16 , 17 , 34 , 36 ) , carcinogenesis, inhibited ( 24 ) , cell cycle regulation ( 29 ) , cell differentiation, induced ( 24 ) , cell growth, induced ( 16 , 17 ) , cell growth, inhibited ( 2 , 14 ) , cell motility, induced ( 13 ) , cytoskeletal reorganization ( 13 ) , signaling pathway regulation ( 29 )

Disease / Diagnostics Relevance
Relevant diseases:	glioblastoma multiforme ( 17 ) , nasopharyngeal carcinoma ( 34 )

References
1	Ke W, et al. (2023) Matrix stiffness induces Drp1-mediated mitochondrial fission through Piezo1 mechanotransduction in human intervertebral disc degeneration. J Transl Med 21, 711 37817199 Curated Info
2	Abu-Hanna J, et al. (2023) Prostacyclin mimetics inhibit DRP1-mediated pro-proliferative mitochondrial fragmentation in pulmonary arterial hypertension. Vascul Pharmacol 151, 107194 37442283 Curated Info
3	Lai YS, et al. (2023) Optogenetically engineered Ca2+ oscillation-mediated DRP1 activation promotes mitochondrial fission and cell death. J Cell Sci 136 37232206 Curated Info
4	Bai YF, et al. (2023) Sevoflurane induces neurotoxic effects on developing neurons through the WNK1/NKCC1/Ca /Drp-1 signalling pathway. Clin Exp Pharmacol Physiol 50, 393-402 36733226 Curated Info
5	Chen W, et al. (2023) TIPE3 represses head and neck squamous cell carcinoma progression via triggering PGAM5 mediated mitochondria dysfunction. Cell Death Dis 14, 251 37024453 Curated Info
6	Ponneri Babuharisankar A, et al. (2023) Mitochondrial Lon-induced mitophagy benefits hypoxic resistance via Ca-dependent FUNDC1 phosphorylation at the ER-mitochondria interface. Cell Death Dis 14, 199 36927870 Curated Info
7	Chen P, et al. (2023) Rab32 promotes glioblastoma migration and invasion via regulation of ERK/Drp1-mediated mitochondrial fission. Cell Death Dis 14, 198 36922509 Curated Info
8	Han L, et al. (2023) Retrograde regulation of mitochondrial fission and epithelial to mesenchymal transition in hepatocellular carcinoma by GCN5L1. Oncogene 36759571 Curated Info
9	Yu Y, et al. (2023) Human umbilical cord mesenchymal stem cell exosome-derived miR-874-3p targeting RIPK1/PGAM5 attenuates kidney tubular epithelial cell damage. Cell Mol Biol Lett 28, 12 36750776 Curated Info
10	Wu Z, et al. (2023) Study on the Key Genes and Molecular Mechanisms of IL-27 Promoting Keratinocytes Proliferation Based on Transcriptome Sequencing. Clin Cosmet Investig Dermatol 16, 1457-1472 37309428 Curated Info
11	Meng Y, et al. (2022) Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy. Signal Transduct Target Ther 7, 388 36481655 Curated Info
12	Gambi G, et al. (2022) The lncRNA LENOX interacts with RAP2C to regulate metabolism and promote resistance to MAPK inhibition in melanoma. Cancer Res 36214632 Curated Info
13	Xiao J, et al. (2022) Caveolin-1 signaling-driven mitochondrial fission and cytoskeleton remodeling promotes breast cancer migration. Int J Biochem Cell Biol 152, 106307 36162640 Curated Info
14	Luo J, et al. (2022) Microsporidia Promote Host Mitochondrial Fragmentation by Modulating DRP1 Phosphorylation. Int J Mol Sci 23 35887094 Curated Info
15	Vijayakumar G, Swetha US, Sudhagar S (2022) Tamoxifen modulates mitochondrial dynamics through AMPK and MAPK during nutrition deprivation. Cell Biol Int 35819094 Curated Info
16	Cai DJ, et al. (2022) Asparagine synthetase regulates lung-cancer metastasis by stabilizing the β-catenin complex and modulating mitochondrial response. Cell Death Dis 13, 566 35739087 Curated Info
17	Miao Z, et al. (2022) Hsp90 induces Acsl4-dependent glioma ferroptosis via dephosphorylating Ser637 at Drp1. Cell Death Dis 13, 548 35697672 Curated Info
18	Xie C, et al. (2022) Mitochondrial micropeptide STMP1 enhances mitochondrial fission to promote tumor metastasis. Cancer Res 82 35544764 Curated Info
19	Tao Z, et al. (2022) Regulating mitochondrial homeostasis and inhibiting inflammatory responses through Celastrol. Ann Transl Med 10, 400 35530963 Curated Info
20	Xiong X, et al. (2022) Activation of Drp1 promotes fatty acids-induced metabolic reprograming to potentiate Wnt signaling in colon cancer. Cell Death Differ 35332310 Curated Info
21	Mao RW, He SP, Lan JG, Zhu WZ (2022) Honokiol ameliorates cisplatin-induced acute kidney injury via inhibition of mitochondrial fission. Br J Pharmacol 35297042 Curated Info
22	Quiring L, et al. (2022) Characterisation of cold-induced mitochondrial fission in porcine aortic endothelial cells. Mol Med 28, 13 35100966 Curated Info
23	Guo J, et al. (2022) Ras-related protein Rab-20 inhibition alleviates cerebral ischemia/reperfusion injury by inhibiting mitochondrial fission and dysfunction. Front Mol Neurosci 15, 986710 36385754 Curated Info
24	Luo M, et al. (2021) Overexpression of carnitine palmitoyltransferase 1A promotes mitochondrial fusion and differentiation of glioblastoma stem cells. Lab Invest 34963686 Curated Info
25	Cha Y, et al. (2021) SIRT2 regulates mitochondrial dynamics and reprogramming via MEK1-ERK-DRP1 and AKT1-DRP1 axes. Cell Rep 37, 110155 34965411 Curated Info
26	Aung LHH, et al. (2021) Cardiomyocyte mitochondrial dynamic-related lncRNA 1 (CMDL-1) may serve as a potential therapeutic target in doxorubicin cardiotoxicity. Mol Ther Nucleic Acids 25, 638-651 34589283 Curated Info
27	Yuan Y, et al. (2021) Activation of ERK-Drp1 signaling promotes hypoxia-induced Aβ accumulation by upregulating mitochondrial fission and BACE1 activity. FEBS Open Bio 11 34403210 Curated Info
28	Volberding PJ, et al. (2021) Suppressive neutrophils require PIM1 for metabolic fitness and survival during chronic viral infection. Cell Rep 35, 109160 34038722 Curated Info
29	Ko HJ, et al. (2021) The Phosphorylation Status of Drp1-Ser637 by PKA in Mitochondrial Fission Modulates Mitophagy via PINK1/Parkin to Exert Multipolar Spindles Assembly during Mitosis. Biomolecules 11 33805672 Curated Info
30	Song SB, Park JS, Jang SY, Hwang ES (2021) Nicotinamide Treatment Facilitates Mitochondrial Fission through Drp1 Activation Mediated by SIRT1-Induced Changes in Cellular Levels of cAMP and Ca. Cells 10 33802063 Curated Info
31	Zhang S, et al. (2021) Recombinant High-Mobility Group Box 1 (rHMGB1) Promotes NRF2-Independent Mitochondrial Fusion through CXCR4/PSMB5-Mediated Drp1 Degradation in Endothelial Cells. Oxid Med Cell Longev 2021, 9993240 34394840 Curated Info
32	Kapetanovic R, et al. (2020) Lipopolysaccharide promotes Drp1-dependent mitochondrial fission and associated inflammatory responses in macrophages. Immunol Cell Biol 32686869 Curated Info
33	Li L, et al. (2020) Shenmai Injection Protects Against Doxorubicin-Induced Cardiotoxicity via Maintaining Mitochondrial Homeostasis. Front Pharmacol 32581790 Curated Info
34	Xie L, et al. (2020) Drp1-dependent remodeling of mitochondrial morphology triggered by EBV-LMP1 increases cisplatin resistance. Signal Transduct Target Ther 5, 56 32433544 Curated Info
35	Simula L, et al. (2020) JNK1 and ERK1/2 modulate lymphocyte homeostasis via BIM and DRP1 upon AICD induction. Cell Death Differ 32346136 Curated Info
36	Chauhan SS, et al. (2020) PIM kinases alter mitochondrial dynamics and chemosensitivity in lung cancer. Oncogene 31992853 Curated Info
37	Wang Y, et al. (2020) Drp1-mediated mitochondrial fission promotes renal fibroblast activation and fibrogenesis. Cell Death Dis 31949126 Curated Info
38	Tomková V, Sandoval-Acuña C, Torrealba N, Truksa J (2019) Mitochondrial fragmentation, elevated mitochondrial superoxide and respiratory supercomplexes disassembly is connected with the tamoxifen-resistant phenotype of breast cancer cells. Free Radic Biol Med 143, 510-521 31494243 Curated Info
39	Drori A, et al. (2018) Cannabinoid-1 receptor regulates mitochondrial dynamics and function in renal proximal tubular cells. Diabetes Obes Metab 30091204 Curated Info
40	NavaneethaKrishnan S, Rosales JL, Lee KY (2018) Loss of Cdk5 in breast cancer cells promotes ROS-mediated cell death through dysregulation of the mitochondrial permeability transition pore. Oncogene 29348461 Curated Info
41	Jhun BS, et al. (2018) Protein kinase D activation induces mitochondrial fragmentation and dysfunction in cardiomyocytes. J Physiol 29313986 Curated Info
42	Li J, et al. (2017) Mitochondrial elongation-mediated glucose metabolism reprogramming is essential for tumour cell survival during energy stress. Oncogene 36, 4901-4912 28436948 Curated Info
43	Cai J, et al. (2016) ERK/Drp1-dependent mitochondrial fission is involved in the MSC-induced drug resistance of T-cell acute lymphoblastic leukemia cells. Cell Death Dis 7, e2459 27831567 Curated Info
44	Dany M, et al. (2016) Targeting FLT3-ITD signaling mediates ceramide-dependent mitophagy and attenuates drug resistance in AML. Blood 128, 1944-1958 27540013 Curated Info
45	Li S, et al. (2016) The 1p36 Tumor Suppressor KIF 1Bβ Is Required for Calcineurin Activation, Controlling Mitochondrial Fission and Apoptosis. Dev Cell 36, 164-78 26812016 Curated Info
46	Xiao L, et al. (2015) Death-associated Protein 3 Regulates Mitochondrial-encoded Protein Synthesis and Mitochondrial Dynamics. J Biol Chem 290, 24961-74 26306039 Curated Info
47	Li G, et al. (2015) Mitochondrial translocation and interaction of cofilin and Drp1 are required for erucin-induced mitochondrial fission and apoptosis. Oncotarget 6, 1834-49 25595902 Curated Info
48	Kim JE, Ryu HJ, Kim MJ, Kang TC (2014) LIM kinase-2 induces programmed necrotic neuronal death via dysfunction of DRP1-mediated mitochondrial fission. Cell Death Differ 21, 1036-49 24561342 Curated Info
49	Cho B, et al. (2014) CDK5-dependent inhibitory phosphorylation of Drp1 during neuronal maturation. Exp Mol Med 46, e105 25012575 Curated Info
50	Wikstrom JD, et al. (2013) AMPK regulates ER morphology and function in stressed pancreatic β-cells via phosphorylation of DRP1. Mol Endocrinol 27, 1706-23 23979843 Curated Info
51	Chou CH, et al. (2012) GSK3beta-Mediated Drp1 Phosphorylation Induced Elongated Mitochondrial Morphology against Oxidative Stress. PLoS One 7, e49112 23185298 Curated Info
52	Rambold AS, Kostelecky B, Elia N, Lippincott-Schwartz J (2011) Tubular network formation protects mitochondria from autophagosomal degradation during nutrient starvation. Proc Natl Acad Sci U S A 108, 10190-5 21646527 Curated Info
53	Cereghetti GM, et al. (2008) Dephosphorylation by calcineurin regulates translocation of Drp1 to mitochondria. Proc Natl Acad Sci U S A 105, 15803-8 18838687 Curated Info
54	Chang CR, Blackstone C (2007) Cyclic AMP-dependent protein kinase phosphorylation of Drp1 regulates its GTPase activity and mitochondrial morphology. J Biol Chem 282, 21583-7 17553808 Curated Info