Thr354
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Home > Phosphorylation Site Page: > Thr354  -  STLK3 (human)

Site Information
IEkLLtRtPDIAQRA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 483899
Associated spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 10 , 11 , 12 , 13 , 14 , 15 , 16 )
Disease tissue studied:
breast cancer ( 4 , 8 ) , breast ductal carcinoma ( 4 ) , HER2 positive breast cancer ( 3 ) , luminal A breast cancer ( 3 ) , luminal B breast cancer ( 3 ) , breast cancer, surrounding tissue ( 3 ) , breast cancer, triple negative ( 3 , 4 ) , liver cancer ( 12 ) , lung cancer ( 8 ) , non-small cell lung cancer ( 8 ) , ovarian cancer ( 4 ) , pancreatic ductal adenocarcinoma ( 5 ) , pancreatic cancer, surrounding tissue ( 13 ) , melanoma skin cancer ( 14 )
Relevant cell line - cell type - tissue:
'pancreatic, ductal'-pancreas ( 5 ) , 293 (epithelial) [AT1 (human), transfection, AT1R stable transfected HEK293] ( 11 ) , brain ( 10 ) , breast ( 3 , 4 ) , BT-20 (breast cell) ( 8 ) , BT-549 (breast cell) ( 8 ) , Calu 6 (pulmonary) ( 8 ) , H2009 (pulmonary) ( 8 ) , H2077 (pulmonary) ( 8 ) , H2887 (pulmonary) ( 8 ) , H322M (pulmonary) ( 8 ) , HCC1359 (pulmonary) ( 8 ) , HCC1937 (breast cell) ( 8 ) , HCC4006 (pulmonary) ( 8 ) , HCC827 (pulmonary) ( 8 ) , HeLa (cervical) ( 2 , 7 , 16 ) , HOP62 (pulmonary) ( 8 ) , Jurkat (T lymphocyte) ( 6 , 15 ) , K562 (erythroid) ( 7 ) , LCLC-103H (pulmonary) ( 8 ) , liver ( 12 ) , MCF-7 (breast cell) ( 8 ) , MDA-MB-231 (breast cell) ( 8 ) , MDA-MB-468 (breast cell) ( 8 ) , NCI-H1395 (pulmonary) ( 8 ) , NCI-H1568 (pulmonary) ( 8 ) , NCI-H157 (pulmonary) ( 8 ) , NCI-H1648 (pulmonary) ( 8 ) , NCI-H1666 (pulmonary) ( 8 ) , NCI-H2030 (pulmonary) ( 8 ) , NCI-H322 (pulmonary) ( 8 ) , NCI-H520 (squamous) ( 8 ) , NCI-H647 (pulmonary) ( 8 ) , ovary ( 4 ) , pancreas ( 13 ) , PC9 (pulmonary) ( 8 ) , skin ( 14 ) , Vero E6-S ('epithelial, kidney') ( 1 )

Upstream Regulation
Putative in vivo kinases:
WNK2 (human) ( 10 )
Treatments:
ischemia ( 4 )

Downstream Regulation
Effects of modification on biological processes:
signaling pathway regulation ( 10 )

References 

1

Bouhaddou M, et al. (2020) The Global Phosphorylation Landscape of SARS-CoV-2 Infection. Cell 182
32645325   Curated Info

2

Huang H, et al. (2016) Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography. Mol Cell Proteomics 15, 3282-3296
27281782   Curated Info

3

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

4

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

5

Britton D, et al. (2014) Quantification of pancreatic cancer proteome and phosphorylome: indicates molecular events likely contributing to cancer and activity of drug targets. PLoS One 9, e90948
24670416   Curated Info

6

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

7

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

8

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

9

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

10

Rinehart J, et al. (2011) WNK2 Kinase Is a Novel Regulator of Essential Neuronal Cation-Chloride Cotransporters. J Biol Chem 286, 30171-80
21733846   Curated Info

11

Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53
20363803   Curated Info

12

Tucker M (2010) CST Curation Set: 9866; Year: 2010; Biosample/Treatment: tissue, liver/untreated; Disease: liver cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

13

Tucker M (2010) CST Curation Set: 9829; Year: 2010; Biosample/Treatment: tissue, pancreas/untreated; Disease: pancreatic cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

14

Tucker M (2010) CST Curation Set: 9781; Year: 2010; Biosample/Treatment: tissue, skin/untreated; Disease: melanoma skin cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

15

Possemato A (2007) CST Curation Set: 3481; Year: 2007; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P Antibodies Used to Purify Peptides prior to LCMS: Phospho-Thr-Pro Motif Antibody (polyAB) Cat#: 3003, PTMScan(R) Phospho-Thr-Pro Motif (T*P) Immunoaffinity Beads Cat#: 1996
Curated Info

16

Beausoleil SA, et al. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization. Nat Biotechnol 24, 1285-92
16964243   Curated Info

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