Tyr185
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Home > Phosphorylation Site Page: > Tyr185  -  JNK1 iso2 (human)

Site Information
TSFMMtPyVVTRYYR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447645

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 5 , 6 ) , phospho-antibody ( 3 , 4 , 7 ) , western blotting ( 3 , 4 , 7 )
Disease tissue studied:
cervical cancer ( 7 ) , cervical squamous cell carcinoma ( 7 ) , lung cancer ( 6 ) , non-small cell lung cancer ( 6 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Kinases, in vitro:
MKK4 (human) ( 7 )
Treatments:
anisomycin ( 7 ) , IL-1a ( 7 ) , osmotic_stress ( 7 ) , thrombin ( 4 ) , TNF ( 7 ) , UV ( 7 )

References 

1

Moritz A (2008) CST Curation Set: 5385; Year: 2008; Biosample/Treatment: cell line, HeLa/nocodazole; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

2

Possemato A (2008) CST Curation Set: 4836; Year: 2008; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

3

Lu C, et al. (2006) Cell apoptosis: requirement of H2AX in DNA ladder formation, but not for the activation of caspase-3. Mol Cell 23, 121-32
16818236   Curated Info

4

Tanabe K, et al. (2005) Midazolam suppresses thrombin-induced heat shock protein 27 phosphorylation through inhibition of p38 mitogen-activated protein kinase in cardiac myocytes. J Cell Biochem 96, 56-64
16052527   Curated Info

5

Gu T (2005) CST Curation Set: 5390; Year: 2005; Biosample/Treatment: cell line, 293T/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

Rikova K (2004) CST Curation Set: 356; Year: 2004; Biosample/Treatment: cell line, NCI-H1703/-; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

7

Fleming Y, et al. (2000) Synergistic activation of stress-activated protein kinase 1/c-Jun N-terminal kinase (SAPK1/JNK) isoforms by mitogen-activated protein kinase kinase 4 (MKK4) and MKK7. Biochem J 352 Pt 1, 145-54
11062067   Curated Info