Tyr185
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Home > Phosphorylation Site Page: > Tyr185  -  JNK1 iso2 (human)

Site Information
TSFMMtPyVVTRYYR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447645

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 2 , 3 , 6 , 7 ) , phospho-antibody ( 4 , 5 , 8 ) , western blotting ( 4 , 5 , 8 )
Disease tissue studied:
cervical cancer ( 8 ) , cervical squamous cell carcinoma ( 8 ) , lung cancer ( 7 ) , non-small cell lung cancer ( 7 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 8 ) , 293T (epithelial) ( 6 ) , HeLa (cervical) ( 2 ) , JB6 RT101 (epidermal) ( 4 ) , Jurkat (T lymphocyte) ( 3 ) , KB (squamous) ( 8 ) , myocyte-heart ( 5 ) , NCI-H1703 (squamous) ( 7 )

Upstream Regulation
Kinases, in vitro:
MKK4 (human) ( 8 )
Treatments:
anisomycin ( 8 ) , IL-1a ( 8 ) , osmotic_stress ( 8 ) , thrombin ( 5 ) , TNF ( 8 ) , UV ( 8 )

References 

1

Chaturvedi A, et al. (2011) Endocytosed BCRs sequentially regulate MAPK and Akt signaling pathways from intracellular compartments. Nat Immunol 12, 1119-26
21964606   Curated Info

2

Moritz A (2008) CST Curation Set: 5385; Year: 2008; Biosample/Treatment: cell line, HeLa/nocodazole; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

3

Possemato A (2008) CST Curation Set: 4836; Year: 2008; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

4

Lu C, et al. (2006) Cell apoptosis: requirement of H2AX in DNA ladder formation, but not for the activation of caspase-3. Mol Cell 23, 121-32
16818236   Curated Info

5

Tanabe K, et al. (2005) Midazolam suppresses thrombin-induced heat shock protein 27 phosphorylation through inhibition of p38 mitogen-activated protein kinase in cardiac myocytes. J Cell Biochem 96, 56-64
16052527   Curated Info

6

Gu T (2005) CST Curation Set: 5390; Year: 2005; Biosample/Treatment: cell line, 293T/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

7

Rikova K (2004) CST Curation Set: 356; Year: 2004; Biosample/Treatment: cell line, NCI-H1703/-; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

8

Fleming Y, et al. (2000) Synergistic activation of stress-activated protein kinase 1/c-Jun N-terminal kinase (SAPK1/JNK) isoforms by mitogen-activated protein kinase kinase 4 (MKK4) and MKK7. Biochem J 352 Pt 1, 145-54
11062067   Curated Info