Ser657
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Home > Phosphorylation Site Page: > Ser657  -  GYS1 (rabbit)

Site Information
RHSsPHQsEDEEEPR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449790

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 2 , 3 ) , electrophoretic mobility shift ( 2 , 3 ) , mutation of modification site ( 1 , 2 , 3 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Kinases, in vitro:
CK2A1 (human) ( 5 , 7 ) , CK2A1 (rabbit) ( 4 , 6 )

Downstream Regulation
Effects of modification on GYS1:
enzymatic activity, inhibited ( 1 , 3 ) , phosphorylation ( 6 )

References 

1

Skurat AV, Dietrich AD, Roach PJ (2000) Glycogen synthase sensitivity to insulin and glucose-6-phosphate is mediated by both NH2- and COOH-terminal phosphorylation sites. Diabetes 49, 1096-100
10909964   Curated Info

2

Skurat AV, Roach PJ (1995) Phosphorylation of sites 3a and 3b (Ser640 and Ser644) in the control of rabbit muscle glycogen synthase. J Biol Chem 270, 12491-7
7759494   Curated Info

3

Skurat AV, Wang Y, Roach PJ (1994) Rabbit skeletal muscle glycogen synthase expressed in COS cells. Identification of regulatory phosphorylation sites. J Biol Chem 269, 25534-42
7929255   Curated Info

4

Wang Y, Roach PJ (1993) Inactivation of rabbit muscle glycogen synthase by glycogen synthase kinase-3. Dominant role of the phosphorylation of Ser-640 (site-3a). J Biol Chem 268, 23876-80
8226927   Curated Info

5

Fiol CJ, et al. (1987) Formation of protein kinase recognition sites by covalent modification of the substrate. Molecular mechanism for the synergistic action of casein kinase II and glycogen synthase kinase 3. J Biol Chem 262, 14042-8
2820993   Curated Info

6

Picton C, Woodgett J, Hemmings B, Cohen P (1982) Multisite phosphorylation of glycogen synthase from rabbit skeletal muscle. Phosphorylation of site 5 by glycogen synthase kinase-5 (casein kinase-II) is a prerequisite for phosphorylation of sites 3 by glycogen synthase kinase-3. FEBS Lett 150, 191-6
6819160   Curated Info

7

Cohen P, et al. (1982) Separation and characterisation of glycogen synthase kinase 3, glycogen synthase kinase 4 and glycogen synthase kinase 5 from rabbit skeletal muscle. Eur J Biochem 124, 21-35
6282589   Curated Info