Ser653
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Home > Phosphorylation Site Page: > Ser653  -  GYS1 (rabbit)

Site Information
PSLsRHSsPHQsEDE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448240

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 2 , 3 ) , electrophoretic mobility shift ( 2 , 3 ) , mutation of modification site ( 1 , 2 , 3 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Kinases, in vitro:
GSK3B (human) ( 5 ) , GSK3B (rabbit) ( 4 )

Downstream Regulation
Effects of modification on GYS1:
enzymatic activity, inhibited ( 1 )

References 

1

Skurat AV, Dietrich AD, Roach PJ (2000) Glycogen synthase sensitivity to insulin and glucose-6-phosphate is mediated by both NH2- and COOH-terminal phosphorylation sites. Diabetes 49, 1096-100
10909964   Curated Info

2

Skurat AV, Roach PJ (1996) Multiple mechanisms for the phosphorylation of C-terminal regulatory sites in rabbit muscle glycogen synthase expressed in COS cells. Biochem J 313 ( Pt 1), 45-50
8546708   Curated Info

3

Skurat AV, Roach PJ (1995) Phosphorylation of sites 3a and 3b (Ser640 and Ser644) in the control of rabbit muscle glycogen synthase. J Biol Chem 270, 12491-7
7759494   Curated Info

4

Wang Y, Roach PJ (1993) Inactivation of rabbit muscle glycogen synthase by glycogen synthase kinase-3. Dominant role of the phosphorylation of Ser-640 (site-3a). J Biol Chem 268, 23876-80
8226927   Curated Info

5

Fiol CJ, et al. (1987) Formation of protein kinase recognition sites by covalent modification of the substrate. Molecular mechanism for the synergistic action of casein kinase II and glycogen synthase kinase 3. J Biol Chem 262, 14042-8
2820993   Curated Info