Ser641
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Home > Phosphorylation Site Page: > Ser641  -  GYS1 (rabbit)

Site Information
YRYPRPAsVPPsPSL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448237

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 1 , 4 , 5 , 6 ) , electrophoretic mobility shift ( 4 , 5 , 6 ) , mutation of modification site ( 1 , 3 , 4 , 5 , 6 ) , phosphopeptide mapping ( 9 , 10 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 9 , 10 ) , COS (fibroblast) ( 4 , 5 , 6 ) , Rat1 (fibroblast) ( 3 )

Upstream Regulation
Putative in vivo kinases:
DYRK1A (mouse) ( 2 ) , DYRK1B (human) ( 2 ) , DYRK2 (human) ( 2 )
Kinases, in vitro:
DYRK1A (human) ( 2 ) , DYRK1B (human) ( 2 ) , GSK3B (human) ( 8 ) , GSK3B (rabbit) ( 7 ) , PASK (human) ( 1 )
Treatments:
gallamine triethiodide ( 10 ) , insulin ( 9 ) , propranolol ( 9 , 10 ) , racepinefrine ( 10 )

Downstream Regulation
Effects of modification on GYS1:
enzymatic activity, inhibited ( 1 , 3 , 4 , 5 , 6 , 7 , 9 )

References 

1

Wilson WA, et al. (2005) Control of mammalian glycogen synthase by PAS kinase. Proc Natl Acad Sci U S A 102, 16596-601
16275910   Curated Info

2

Skurat AV, Dietrich AD (2004) Phosphorylation of Ser640 in muscle glycogen synthase by DYRK family protein kinases. J Biol Chem 279, 2490-8
14593110   Curated Info

3

Skurat AV, Dietrich AD, Roach PJ (2000) Glycogen synthase sensitivity to insulin and glucose-6-phosphate is mediated by both NH2- and COOH-terminal phosphorylation sites. Diabetes 49, 1096-100
10909964   Curated Info

4

Skurat AV, Roach PJ (1996) Multiple mechanisms for the phosphorylation of C-terminal regulatory sites in rabbit muscle glycogen synthase expressed in COS cells. Biochem J 313 ( Pt 1), 45-50
8546708   Curated Info

5

Skurat AV, Roach PJ (1995) Phosphorylation of sites 3a and 3b (Ser640 and Ser644) in the control of rabbit muscle glycogen synthase. J Biol Chem 270, 12491-7
7759494   Curated Info

6

Skurat AV, Wang Y, Roach PJ (1994) Rabbit skeletal muscle glycogen synthase expressed in COS cells. Identification of regulatory phosphorylation sites. J Biol Chem 269, 25534-42
7929255   Curated Info

7

Wang Y, Roach PJ (1993) Inactivation of rabbit muscle glycogen synthase by glycogen synthase kinase-3. Dominant role of the phosphorylation of Ser-640 (site-3a). J Biol Chem 268, 23876-80
8226927   Curated Info

8

Fiol CJ, et al. (1987) Formation of protein kinase recognition sites by covalent modification of the substrate. Molecular mechanism for the synergistic action of casein kinase II and glycogen synthase kinase 3. J Biol Chem 262, 14042-8
2820993   Curated Info

9

Parker PJ, Caudwell FB, Cohen P (1983) Glycogen synthase from rabbit skeletal muscle; effect of insulin on the state of phosphorylation of the seven phosphoserine residues in vivo. Eur J Biochem 130, 227-34
6402364   Curated Info

10

Parker PJ, Embi N, Caudwell FB, Cohen P (1982) Glycogen synthase from rabbit skeletal muscle. State of phosphorylation of the seven phosphoserine residues in vivo in the presence and absence of adrenaline. Eur J Biochem 124, 47-55
6211353   Curated Info