Tyr861
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Home > Phosphorylation Site Page: > Tyr861  -  FAK (mouse)

Site Information
PTGNQHIyQPVGKPD   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447965

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 2 ) , mutation of modification site ( 4 , 5 ) , peptide sequencing ( 7 ) , phospho-antibody ( 3 , 4 , 5 , 6 , 7 , 8 ) , western blotting ( 3 , 4 , 5 , 7 )
Disease tissue studied:
breast cancer ( 4 ) , fibrosarcoma ( 7 ) , liver cancer ( 5 ) , cholangiocellular carcinoma ( 5 )
Relevant cell line - cell type - tissue:
4T1 (breast cell) ( 4 ) , IEC-18 (epithelial) ( 3 ) , lung ( 6 ) , MCA102 (fibroblast) ( 7 ) , MCA102 (fibroblast) [BST1 (mouse)] ( 7 ) , MEF (fibroblast) ( 2 , 4 , 5 ) , MEF (fibroblast) [PTP1B (mouse), homozygous knockout] ( 2 ) , NMuMG (epithelial) ( 8 ) , SYF (fibroblast) [Src (mouse)] ( 3 )

Upstream Regulation
Regulatory protein:
BST1 (mouse) ( 7 ) , FAK iso9 (mouse) ( 4 ) , Src (mouse) ( 3 )
Kinases, in vitro:
Src (human) ( 9 )
Treatments:
angiotensin_2 ( 3 ) , cytochalasin_D ( 3 ) , latrunculin_A ( 3 ) , low_cell_density ( 8 ) , LPA ( 3 ) , PP2 ( 3 ) , sorbitol ( 3 ) , SU6656 ( 3 ) , sucrose ( 3 ) , TGF-beta ( 8 ) , TNF ( 5 ) , urea ( 3 ) , VEGF ( 6 )

Downstream Regulation
Effects of modification on FAK:
phosphorylation ( 3 )
Effects of modification on biological processes:
cytoskeletal reorganization ( 3 )

References 

1

Hsieh MY, et al. (2014) The Inducible Nitric-oxide Synthase (iNOS)/Src Axis Mediates Toll-like Receptor 3 Tyrosine 759 Phosphorylation and Enhances Its Signal Transduction, Leading to Interferon-β Synthesis in Macrophages. J Biol Chem 289, 9208-20
24526685   Curated Info

2

Mertins P, et al. (2008) Investigation of protein-tyrosine phosphatase 1B function by quantitative proteomics. Mol Cell Proteomics 7, 1763-77
18515860   Curated Info

3

Lunn JA, Jacamo R, Rozengurt E (2007) Preferential phosphorylation of focal adhesion kinase tyrosine 861 is critical for mediating an anti-apoptotic response to hyperosmotic stress. J Biol Chem 282, 10370-9
17289681   Curated Info

4

Mitra SK, et al. (2006) Intrinsic FAK activity and Y925 phosphorylation facilitate an angiogenic switch in tumors. Oncogene 25, 5969-84
16682956   Curated Info

5

Mon NN, et al. (2006) A role for focal adhesion kinase signaling in tumor necrosis factor-alpha-dependent matrix metalloproteinase-9 production in a cholangiocarcinoma cell line, CCKS1. Cancer Res 66, 6778-84
16818654   Curated Info

6

Eliceiri BP, et al. (2002) Src-mediated coupling of focal adhesion kinase to integrin alpha(v)beta5 in vascular endothelial growth factor signaling. J Cell Biol 157, 149-60
11927607   Curated Info

7

Liang F, Qi RZ, Chang CF (2001) Signalling of GPI-anchored CD157 via focal adhesion kinase in MCA102 fibroblasts. FEBS Lett 506, 207-10
11602246   Curated Info

8

Nakamura K, Yano H, Schaefer E, Sabe H (2001) Different modes and qualities of tyrosine phosphorylation of Fak and Pyk2 during epithelial-mesenchymal transdifferentiation and cell migration: analysis of specific phosphorylation events using site-directed antibodies. Oncogene 20, 2626-35
11420674   Curated Info

9

Calalb MB, Zhang X, Polte TR, Hanks SK (1996) Focal adhesion kinase tyrosine-861 is a major site of phosphorylation by Src. Biochem Biophys Res Commun 228, 662-8
8941336   Curated Info