Tyr576
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Home > Phosphorylation Site Page: > Tyr576  -  FAK (mouse)

Site Information
RyMEDstyyKASKGK   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447889
Associated spectra:  2 CST

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 14 ) , [32P] bio-synthetic labeling ( 13 , 14 ) , immunoassay ( 1 ) , immunoprecipitation ( 12 , 13 , 14 ) , mass spectrometry ( 2 , 3 , 5 ) , mutation of modification site ( 8 , 9 , 12 , 13 , 14 ) , phospho-antibody ( 1 , 2 , 4 , 6 , 7 , 8 , 9 , 10 , 11 ) , phosphoamino acid analysis ( 13 , 14 ) , phosphopeptide mapping ( 13 , 14 ) , western blotting ( 1 , 2 , 4 , 6 , 7 , 8 , 9 , 12 )
Disease tissue studied:
breast cancer ( 8 ) , liver cancer ( 1 , 9 ) , cholangiocellular carcinoma ( 9 )
Relevant cell line - cell type - tissue:
'fat, brown' ( 3 ) , 293 (epithelial) ( 13 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 13 , 14 ) , 4T1 (breast cell) ( 8 ) , B lymphocyte ( 2 ) , brain ( 3 ) , C3H10T1/2 (fibroblast) ( 7 ) , COS (fibroblast) ( 11 , 12 , 14 ) , hepatocyte-liver ( 1 ) , HepG2 (hepatic) ( 1 ) , IEC-18 (epithelial) ( 6 ) , kidney ( 3 ) , LO2 (hepatocyte) ( 1 ) , lung ( 3 ) , MEF (fibroblast) [FAK iso2 (mouse), transgenic] ( 4 ) , MEF (fibroblast) [PTP1B (mouse), homozygous knockout] ( 5 ) , MEF (fibroblast) ( 5 , 8 , 9 ) , NMuMG (epithelial) ( 10 ) , SMMC7721 (hepatocyte) ( 1 ) , SYF (fibroblast) [Src iso1 (mouse)] ( 6 )

Upstream Regulation
Regulatory protein:
FAK iso2 (mouse) ( 4 ) , FAK iso9 (mouse) ( 8 ) , IFITM3 (human) ( 2 ) , IFITM3 (rat) ( 2 ) , Src iso1 (mouse) ( 6 )
Putative in vivo kinases:
Src iso1 (mouse) ( 11 , 12 , 14 )
Kinases, in vitro:
Src (human) ( 14 )
Putative upstream phosphatases:
DUSP22 (human) ( 1 )
Treatments:
angiotensin_2 ( 6 ) , cytochalasin_D ( 6 ) , dexamethasone ( 7 ) , EGF ( 7 ) , fibronectin ( 4 , 11 , 13 ) , HFHC ( 1 ) , IBMX ( 7 ) , latrunculin_A ( 6 ) , low_cell_density ( 10 ) , LPA ( 6 ) , PP2 ( 7 ) , siRNA ( 1 ) , sorbitol ( 6 ) , sucrose ( 6 ) , TCDD ( 7 ) , TGF-beta ( 10 ) , TNF ( 9 ) , U0126 ( 7 )

Downstream Regulation
Effects of modification on FAK:
activity, induced ( 11 , 14 ) , molecular association, regulation ( 12 )
Effects of modification on biological processes:
carcinogenesis, induced ( 1 ) , cell adhesion, altered ( 12 ) , cell growth, induced ( 1 ) , signaling pathway regulation ( 1 ) , transcription, altered ( 1 )
Induce interaction with:
P130Cas (mouse) ( 12 )

Disease / Diagnostics Relevance
Relevant diseases:
liver cancer ( 1 ) , hepatitis ( 1 )

References 

1

Ge C, et al. (2022) Hepatocyte phosphatase DUSP22 mitigates NASH-HCC progression by targeting FAK. Nat Commun 13, 5945
36209205   Curated Info

2

Lee J, et al. (2020) IFITM3 functions as a PIP3 scaffold to amplify PI3K signalling in B cells. Nature 588
33149299   Curated Info

3

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

4

Corsi JM, et al. (2009) Autophosphorylation-independent and -dependent functions of focal adhesion kinase during development. J Biol Chem 284, 34769-76
19776009   Curated Info

5

Mertins P, et al. (2008) Investigation of protein-tyrosine phosphatase 1B function by quantitative proteomics. Mol Cell Proteomics 7, 1763-77
18515860   Curated Info

6

Lunn JA, Jacamo R, Rozengurt E (2007) Preferential phosphorylation of focal adhesion kinase tyrosine 861 is critical for mediating an anti-apoptotic response to hyperosmotic stress. J Biol Chem 282, 10370-9
17289681   Curated Info

7

Liu X, Jefcoate C (2006) 2,3,7,8-tetrachlorodibenzo-p-dioxin and epidermal growth factor cooperatively suppress peroxisome proliferator-activated receptor-gamma1 stimulation and restore focal adhesion complexes during adipogenesis: selective contributions of Src, Rho, and Erk distinguish these overlapping processes in C3H10T1/2 cells. Mol Pharmacol 70, 1902-15
16971554   Curated Info

8

Mitra SK, et al. (2006) Intrinsic FAK activity and Y925 phosphorylation facilitate an angiogenic switch in tumors. Oncogene 25, 5969-84
16682956   Curated Info

9

Mon NN, et al. (2006) A role for focal adhesion kinase signaling in tumor necrosis factor-alpha-dependent matrix metalloproteinase-9 production in a cholangiocarcinoma cell line, CCKS1. Cancer Res 66, 6778-84
16818654   Curated Info

10

Nakamura K, Yano H, Schaefer E, Sabe H (2001) Different modes and qualities of tyrosine phosphorylation of Fak and Pyk2 during epithelial-mesenchymal transdifferentiation and cell migration: analysis of specific phosphorylation events using site-directed antibodies. Oncogene 20, 2626-35
11420674   Curated Info

11

Ruest PJ, et al. (2000) Phosphospecific antibodies reveal focal adhesion kinase activation loop phosphorylation in nascent and mature focal adhesions and requirement for the autophosphorylation site. Cell Growth Differ 11, 41-8
10672902   Curated Info

12

Polte TR, Hanks SK (1997) Complexes of focal adhesion kinase (FAK) and Crk-associated substrate (p130(Cas)) are elevated in cytoskeleton-associated fractions following adhesion and Src transformation. Requirements for Src kinase activity and FAK proline-rich motifs. J Biol Chem 272, 5501-9
9038154   Curated Info

13

Schlaepfer DD, Hunter T (1996) Evidence for in vivo phosphorylation of the Grb2 SH2-domain binding site on focal adhesion kinase by Src-family protein-tyrosine kinases. Mol Cell Biol 16, 5623-33
8816475   Curated Info

14

Calalb MB, Polte TR, Hanks SK (1995) Tyrosine phosphorylation of focal adhesion kinase at sites in the catalytic domain regulates kinase activity: a role for Src family kinases. Mol Cell Biol 15, 954-63
7529876   Curated Info

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