Tyr397
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Home > Phosphorylation Site Page: > Tyr397  -  FAK (mouse)

Site Information
sVsEtDDyAEIIDEE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447887
Associated spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 88 ) , [32P] bio-synthetic labeling ( 87 , 88 ) , immunoprecipitation ( 2 , 39 , 87 , 88 ) , mass spectrometry ( 5 , 6 , 7 , 8 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 19 , 20 , 21 , 22 , 24 , 25 , 26 , 27 , 28 , 30 , 32 , 33 , 34 , 35 , 36 , 37 , 40 , 41 , 43 , 44 , 45 , 48 , 50 , 52 , 53 , 54 , 55 , 56 , 58 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 72 , 73 , 74 , 75 , 76 , 78 ) , mutation of modification site ( 2 , 29 , 39 , 46 , 47 , 49 , 77 , 85 , 86 , 87 , 88 ) , peptide sequencing ( 80 ) , phospho-antibody ( 1 , 2 , 3 , 9 , 17 , 18 , 23 , 29 , 31 , 38 , 39 , 42 , 46 , 47 , 49 , 51 , 57 , 59 , 60 , 61 , 71 , 77 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 ) , phosphoamino acid analysis ( 87 , 88 ) , phosphopeptide mapping ( 87 , 88 ) , western blotting ( 1 , 2 , 3 , 9 , 17 , 18 , 23 , 29 , 31 , 38 , 39 , 42 , 46 , 49 , 51 , 57 , 59 , 60 , 61 , 71 , 77 , 80 , 81 , 83 , 85 , 86 )
Disease tissue studied:
breast cancer ( 29 , 46 ) , fibrosarcoma ( 80 ) , liver cancer ( 49 ) , cholangiocellular carcinoma ( 49 ) , lung cancer ( 24 , 25 , 26 , 27 , 28 ) , non-small cell lung cancer ( 24 , 25 , 26 , 27 , 28 ) , melanoma skin cancer ( 83 ) , testicular cancer ( 7 , 61 )
Relevant cell line - cell type - tissue:
'stem, mesenchymal' ( 30 ) , 293 (epithelial) ( 71 , 86 , 87 ) , 3T3 (fibroblast) [Pyk2 (human), transfection, stable Pyk2 (v5-His tagged) transfection, in RU-486 (mifepristone)-inducible expression system] ( 6 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 59 , 85 , 87 , 88 ) , 3T3 (fibroblast) [Src (human)] ( 58 ) , 3T3 (fibroblast) ( 39 , 52 , 53 , 54 , 55 , 56 , 58 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 72 , 73 , 74 , 75 , 76 , 77 , 78 ) , 3T3-L1 (fibroblast) ( 48 ) , 4T1 (breast cell) ( 46 ) , B16 (melanocyte) ( 83 ) , BaF3 ('B lymphocyte, precursor') ( 50 ) , C2C12 (myoblast) ( 11 , 12 , 13 , 14 , 15 , 57 ) , C2C12-D (muscle cell) ( 36 , 37 , 40 , 41 ) , C3H10T1/2 (fibroblast) ( 19 , 20 , 21 , 22 ) , CHO (fibroblast) ( 71 ) , COS (fibroblast) ( 77 , 84 , 88 ) , EpH4 (epithelial) ( 1 ) , F9 (testicular) ( 61 ) , HEK293T (epithelial) ( 85 ) , IEC-18 (epithelial) ( 38 ) , lens ( 42 ) , liver ( 34 , 35 ) , liver [Akt2 (mouse), homozygous knockout] ( 32 , 33 ) , lung ( 24 , 25 , 26 , 27 , 43 , 44 , 45 , 79 ) , MCA102 (fibroblast) ( 80 ) , MCA102 (fibroblast) [BST1 (mouse)] ( 80 ) , MDA-MB-435 (breast cell) ( 29 ) , MEF (fibroblast) ( 2 , 10 , 16 , 17 , 18 , 23 , 31 , 46 , 47 , 49 , 51 ) , MEF (fibroblast) [FAK iso2 (mouse), transgenic] ( 9 ) , MEF (fibroblast) [PTP1B (mouse), homozygous knockout] ( 10 ) , MEF-3T3 ( 3 ) , MMTV-PyMT (breast cell) ( 29 ) , MS1 (endothelial) ( 57 ) , myocyte-heart ( 18 ) , NMuMG (epithelial) ( 82 ) , P19 (testicular) ( 7 ) , PYS-2 (epithelial) ( 8 ) , Swiss 3T3 (fibroblast) ( 51 , 59 , 60 , 81 ) , SYF (fibroblast) [Src (mouse), transfection] ( 5 ) , SYF (fibroblast) [Src (mouse)] ( 38 ) , SYF (fibroblast) ( 18 , 81 )

Upstream Regulation
Regulatory protein:
BST1 (mouse) ( 80 ) , dematin (mouse) ( 17 ) , FAK iso2 (mouse) ( 9 ) , FAK iso9 (mouse) ( 46 ) , MGAT5 (mouse) ( 29 ) , MYO1E (mouse) ( 2 ) , PAG (rat) ( 23 ) , PXN (mouse) ( 3 ) , Src (mouse) ( 38 ) , TGFBR2 (mouse) ( 42 ) , Vinculin (mouse) ( 61 )
Putative in vivo kinases:
FAK iso2 (mouse) ( 88 ) , Src (mouse) ( 81 )
Kinases, in vitro:
FAK iso2 (mouse) ( 88 )
Treatments:
angiopoietin-1 ( 57 ) , angiopoietin-2 ( 57 ) , angiotensin_2 ( 38 ) , bombesin ( 51 , 81 ) , bradykinin ( 51 , 81 ) , calphostin_C ( 85 ) , cell_adhesion ( 17 , 31 ) , chloroquine ( 39 ) , Clostridium_toxin_B ( 59 ) , cytochalasin_D ( 38 , 60 , 85 ) , deferoxamine ( 39 ) , echistatin ( 83 ) , endothelin ( 81 ) , FGF1 ( 60 ) , fibronectin ( 9 , 31 , 71 , 77 , 81 , 85 , 86 , 87 ) , genistein ( 85 ) , H2O2 ( 18 ) , herbimycin_A ( 85 ) , high_cell_density ( 39 ) , laminin-1 ( 57 ) , latrunculin_A ( 38 ) , low_cell_density ( 82 ) , LPA ( 38 , 81 ) , LY294002 ( 59 , 60 ) , osmotic_stress ( 59 ) , PDGF ( 60 ) , phorbol_ester ( 85 ) , poly-L-lysine ( 85 ) , PP1 ( 18 ) , PP2 ( 59 , 81 ) , SB202190 ( 59 ) , SB203580 ( 59 ) , serum_starvation ( 39 , 85 ) , siRNA ( 39 ) , sorbitol ( 38 ) , sucrose ( 38 ) , TGF-beta ( 82 ) , thapsigargin ( 51 ) , TNF ( 49 ) , tyrphostin_A47 ( 85 ) , U0126 ( 60 ) , vasopressin ( 51 , 81 ) , VEGF ( 79 ) , vitronectin ( 57 ) , wortmannin ( 60 ) , Y27632 ( 17 , 59 )

Downstream Regulation
Effects of modification on FAK:
activity, induced ( 84 ) , enzymatic activity, induced ( 86 ) , intracellular localization ( 2 ) , molecular association, regulation ( 2 , 31 , 77 , 86 , 87 ) , phosphorylation ( 86 ) , protein degradation ( 77 )
Effects of modification on biological processes:
cell adhesion, altered ( 9 ) , cell growth, altered ( 9 ) , cell motility, altered ( 31 ) , cytoskeletal reorganization ( 9 ) , transcription, altered ( 49 ) , transcription, induced ( 2 )
Induce interaction with:
GRB2 (human) ( 86 , 87 ) , P130Cas (human) ( 86 ) , SHC1 (human) ( 86 ) , SOCS1 (mouse) ( 77 ) , SOCS3 (mouse) ( 77 ) , Src (human) ( 86 , 87 ) , Src (mouse) ( 2 )
Inhibit interaction with:
Arp3 (mouse) ( 31 )

References 

1

Nakatsu D, Kano F, Shinozaki-Narikawa N, Murata M (2019) Pyk2-dependent phosphorylation of LSR enhances localization of LSR and tricellulin at tricellular tight junctions. PLoS One 14, e0223300
31574128   Curated Info

2

Heim JB, et al. (2017) Myosin-1E interacts with FAK proline-rich region 1 to induce fibronectin-type matrix. Proc Natl Acad Sci U S A 114, 3933-3938
28348210   Curated Info

3

Quizi JL, et al. (2013) SLK-mediated phosphorylation of paxillin is required for focal adhesion turnover and cell migration. Oncogene 32, 4656-63
23128389   Curated Info

4

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

5

Ferrando IM, et al. (2012) Identification of targets of c-Src tyrosine kinase by chemical complementation and phosphoproteomics. Mol Cell Proteomics 11, 355-69
22499769   Curated Info

6

Bonnette PC, et al. (2010) Phosphoproteomic characterization of PYK2 signaling pathways involved in osteogenesis. J Proteomics 73, 1306-20
20116462   Curated Info

7

Zhou J (2010) CST Curation Set: 9109; Year: 2010; Biosample/Treatment: cell line, P19/untreated; Disease: testicular cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-102) Cat#: 9416
Curated Info

8

Zhou J (2010) CST Curation Set: 9108; Year: 2010; Biosample/Treatment: cell line, PY2/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-102) Cat#: 9416
Curated Info

9

Corsi JM, et al. (2009) Autophosphorylation-independent and -dependent functions of focal adhesion kinase during development. J Biol Chem 284, 34769-76
19776009   Curated Info

10

Mertins P, et al. (2008) Investigation of protein-tyrosine phosphatase 1B function by quantitative proteomics. Mol Cell Proteomics 7, 1763-77
18515860   Curated Info

11

Possemato A (2008) CST Curation Set: 4931; Year: 2008; Biosample/Treatment: cell line, C2C12/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

12

Possemato A (2008) CST Curation Set: 4932; Year: 2008; Biosample/Treatment: cell line, C2C12/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

13

Possemato A (2008) CST Curation Set: 4933; Year: 2008; Biosample/Treatment: cell line, C2C12/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

14

Possemato A (2008) CST Curation Set: 4934; Year: 2008; Biosample/Treatment: cell line, C2C12/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

15

Possemato A (2008) CST Curation Set: 4929; Year: 2008; Biosample/Treatment: cell line, C2C12/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

16

Luo W, et al. (2008) Global impact of oncogenic Src on a phosphotyrosine proteome. J Proteome Res 7, 3447-60
18563927   Curated Info

17

Mohseni M, Chishti AH (2008) The headpiece domain of dematin regulates cell shape, motility, and wound healing by modulating RhoA activation. Mol Cell Biol 28, 4712-8
18505823   Curated Info

18

Rybin VO, et al. (2008) Phorbol 12-myristate 13-acetate-dependent protein kinase C delta-Tyr311 phosphorylation in cardiomyocyte caveolae. J Biol Chem 283, 17777-88
18387943   Curated Info

19

Li Y (2008) CST Curation Set: 4544; Year: 2008; Biosample/Treatment: cell line, 10T1/2/FGF; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

20

Li Y (2008) CST Curation Set: 4543; Year: 2008; Biosample/Treatment: cell line, 10T1/2/FGF; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

21

Li Y (2008) CST Curation Set: 4542; Year: 2008; Biosample/Treatment: cell line, 10T1/2/FGF; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

22

Li Y (2008) CST Curation Set: 4541; Year: 2008; Biosample/Treatment: cell line, 10T1/2/control; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

23

Oneyama C, et al. (2008) The lipid raft-anchored adaptor protein Cbp controls the oncogenic potential of c-Src. Mol Cell 30, 426-36
18498747   Curated Info

24

Rikova K (2008) CST Curation Set: 4465; Year: 2008; Biosample/Treatment: tissue, lung/untreated; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

25

Rikova K (2008) CST Curation Set: 4464; Year: 2008; Biosample/Treatment: tissue, lung/untreated; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

26

Rikova K (2008) CST Curation Set: 4439; Year: 2008; Biosample/Treatment: tissue, lung/untreated; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

27

Rikova K (2008) CST Curation Set: 4440; Year: 2008; Biosample/Treatment: tissue, lung/untreated; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

28

Rikova K (2008) CST Curation Set: 4254; Year: 2008; Biosample/Treatment: tissue, lung/untreated; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

29

Goetz JG, et al. (2008) Concerted regulation of focal adhesion dynamics by galectin-3 and tyrosine-phosphorylated caveolin-1. J Cell Biol 180, 1261-75
18347068   Curated Info

30

Yu J (2007) CST Curation Set: 3380; Year: 2007; Biosample/Treatment: cell line, Mouse Mesenchymal Stem Cells/bFGF; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

31

Serrels B, et al. (2007) Focal adhesion kinase controls actin assembly via a FERM-mediated interaction with the Arp2/3 complex. Nat Cell Biol 9, 1046-56
17721515   Curated Info

32

Guo A (2007) CST Curation Set: 2988; Year: 2007; Biosample/Treatment: tissue, liver/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

33

Guo A (2007) CST Curation Set: 2989; Year: 2007; Biosample/Treatment: tissue, liver/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

34

Guo A (2007) CST Curation Set: 2983; Year: 2007; Biosample/Treatment: tissue, liver/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

35

Guo A (2007) CST Curation Set: 2986; Year: 2007; Biosample/Treatment: tissue, liver/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

36

Guo A (2007) CST Curation Set: 2782; Year: 2007; Biosample/Treatment: cell line, C2C12-D/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

37

Guo A (2007) CST Curation Set: 2783; Year: 2007; Biosample/Treatment: cell line, C2C12-D/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

38

Lunn JA, Jacamo R, Rozengurt E (2007) Preferential phosphorylation of focal adhesion kinase tyrosine 861 is critical for mediating an anti-apoptotic response to hyperosmotic stress. J Biol Chem 282, 10370-9
17289681   Curated Info

39

Lim Y, et al. (2007) Focal adhesion kinase is negatively regulated by phosphorylation at tyrosine 407. J Biol Chem 282, 10398-404
17303567   Curated Info

40

Guo A (2007) CST Curation Set: 2564; Year: 2007; Biosample/Treatment: cell line, C2C12-D/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

41

Guo A (2007) CST Curation Set: 2565; Year: 2007; Biosample/Treatment: cell line, C2C12-D/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

42

Kokkinos MI, Brown HJ, de Iongh RU (2007) Focal adhesion kinase (FAK) expression and activation during lens development. Mol Vis 13, 418-30
17417603   Curated Info

43

Farnsworth C (2006) CST Curation Set: 2092; Year: 2006; Biosample/Treatment: tissue, lung/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

44

Farnsworth C (2006) CST Curation Set: 2093; Year: 2006; Biosample/Treatment: tissue, lung/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

45

Farnsworth C (2006) CST Curation Set: 2094; Year: 2006; Biosample/Treatment: tissue, lung/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

46

Mitra SK, et al. (2006) Intrinsic FAK activity and Y925 phosphorylation facilitate an angiogenic switch in tumors. Oncogene 25, 5969-84
16682956   Curated Info

47

Gaus K, Le Lay S, Balasubramanian N, Schwartz MA (2006) Integrin-mediated adhesion regulates membrane order. J Cell Biol 174, 725-34
16943184   Curated Info

48

Schmelzle K, et al. (2006) Temporal dynamics of tyrosine phosphorylation in insulin signaling. Diabetes 55, 2171-9
16873679   Curated Info

49

Mon NN, et al. (2006) A role for focal adhesion kinase signaling in tumor necrosis factor-alpha-dependent matrix metalloproteinase-9 production in a cholangiocarcinoma cell line, CCKS1. Cancer Res 66, 6778-84
16818654   Curated Info

50

Goss V (2005) CST Curation Set: 847; Year: 2005; Biosample/Treatment: cell line, BaF3/serum starved; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

51

Fan RS, et al. (2005) G protein-coupled receptor activation rapidly stimulates focal adhesion kinase phosphorylation at Ser-843. Mediation by Ca2+, calmodulin, and Ca2+/calmodulin-dependent kinase II. J Biol Chem 280, 24212-20
15845548   Curated Info

52

Guo A (2005) CST Curation Set: 629; Year: 2005; Biosample/Treatment: cell line, 3T3/serum starved; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

53

Guo A (2005) CST Curation Set: 630; Year: 2005; Biosample/Treatment: cell line, 3T3/EGF; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

54

Guo A (2005) CST Curation Set: 631; Year: 2005; Biosample/Treatment: cell line, 3T3/serum starved; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

55

Guo A (2005) CST Curation Set: 632; Year: 2005; Biosample/Treatment: cell line, 3T3/EGF; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

56

Guo A (2005) CST Curation Set: 634; Year: 2005; Biosample/Treatment: cell line, 3T3/EGF; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

57

Dallabrida SM, et al. (2005) Angiopoietin-1 promotes cardiac and skeletal myocyte survival through integrins. Circ Res 96, e8-24
15692086   Curated Info

58

Rush J, et al. (2005) Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. Nat Biotechnol 23, 94-101
15592455   Curated Info

59

Lunn JA, Rozengurt E (2004) Hyperosmotic stress induces rapid focal adhesion kinase phosphorylation at tyrosines 397 and 577. Role of Src family kinases and Rho family GTPases. J Biol Chem 279, 45266-78
15302877   Curated Info

60

Hunger-Glaser I, Fan RS, Perez-Salazar E, Rozengurt E (2004) PDGF and FGF induce focal adhesion kinase (FAK) phosphorylation at Ser-910: dissociation from Tyr-397 phosphorylation and requirement for ERK activation. J Cell Physiol 200, 213-22
15174091   Curated Info

61

Subauste MC, et al. (2004) Vinculin modulation of paxillin-FAK interactions regulates ERK to control survival and motility. J Cell Biol 165, 371-81
15138291   Curated Info

62

Moritz A (2004) CST Curation Set: 261; Year: 2004; Biosample/Treatment: cell line, 3T3/serum starved; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

63

Moritz A (2004) CST Curation Set: 263; Year: 2004; Biosample/Treatment: cell line, 3T3/serum starved; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

64

Moritz A (2004) CST Curation Set: 264; Year: 2004; Biosample/Treatment: cell line, 3T3/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

65

Moritz A (2004) CST Curation Set: 265; Year: 2004; Biosample/Treatment: cell line, 3T3/serum starved; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

66

Moritz A (2003) CST Curation Set: 207; Year: 2003; Biosample/Treatment: cell line, 3T3/NRG1; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

67

Moritz A (2003) CST Curation Set: 208; Year: 2003; Biosample/Treatment: cell line, 3T3/NRG1; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

68

Moritz A (2003) CST Curation Set: 209; Year: 2003; Biosample/Treatment: cell line, 3T3/NRG1; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

69

Moritz A (2003) CST Curation Set: 210; Year: 2003; Biosample/Treatment: cell line, 3T3/NRG1; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

70

Moritz A (2003) CST Curation Set: 211; Year: 2003; Biosample/Treatment: cell line, 3T3/NRG1; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

71

Cooper LA, Shen TL, Guan JL (2003) Regulation of focal adhesion kinase by its amino-terminal domain through an autoinhibitory interaction. Mol Cell Biol 23, 8030-41
14585964   Curated Info

72

Moritz A (2003) CST Curation Set: 148; Year: 2003; Biosample/Treatment: cell line, 3T3/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

73

Moritz A (2003) CST Curation Set: 149; Year: 2003; Biosample/Treatment: cell line, 3T3/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

74

Moritz A (2003) CST Curation Set: 150; Year: 2003; Biosample/Treatment: cell line, 3T3/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

75

Moritz A (2003) CST Curation Set: 151; Year: 2003; Biosample/Treatment: cell line, 3T3/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

76

Moritz A (2003) CST Curation Set: 152; Year: 2003; Biosample/Treatment: cell line, 3T3/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

77

Liu E, Côté JF, Vuori K (2003) Negative regulation of FAK signaling by SOCS proteins. EMBO J 22, 5036-46
14517242   Curated Info

78

Moritz A (2003) CST Curation Set: 88; Year: 2003; Biosample/Treatment: cell line, 3T3/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

79

Eliceiri BP, et al. (2002) Src-mediated coupling of focal adhesion kinase to integrin alpha(v)beta5 in vascular endothelial growth factor signaling. J Cell Biol 157, 149-60
11927607   Curated Info

80

Liang F, Qi RZ, Chang CF (2001) Signalling of GPI-anchored CD157 via focal adhesion kinase in MCA102 fibroblasts. FEBS Lett 506, 207-10
11602246   Curated Info

81

Salazar EP, Rozengurt E (2001) Src family kinases are required for integrin-mediated but not for G protein-coupled receptor stimulation of focal adhesion kinase autophosphorylation at Tyr-397. J Biol Chem 276, 17788-95
11279163   Curated Info

82

Nakamura K, Yano H, Schaefer E, Sabe H (2001) Different modes and qualities of tyrosine phosphorylation of Fak and Pyk2 during epithelial-mesenchymal transdifferentiation and cell migration: analysis of specific phosphorylation events using site-directed antibodies. Oncogene 20, 2626-35
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83

Della Morte R, et al. (2000) Echistatin inhibits pp125FAK autophosphorylation, paxillin phosphorylation and pp125FAK-paxillin interaction in fibronectin-adherent melanoma cells. Eur J Biochem 267, 5047-54
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84

Ruest PJ, et al. (2000) Phosphospecific antibodies reveal focal adhesion kinase activation loop phosphorylation in nascent and mature focal adhesions and requirement for the autophosphorylation site. Cell Growth Differ 11, 41-8
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85

Schlaepfer DD, Jones KC, Hunter T (1998) Multiple Grb2-mediated integrin-stimulated signaling pathways to ERK2/mitogen-activated protein kinase: summation of both c-Src- and focal adhesion kinase-initiated tyrosine phosphorylation events. Mol Cell Biol 18, 2571-85
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86

Schlaepfer DD, Hunter T (1997) Focal adhesion kinase overexpression enhances ras-dependent integrin signaling to ERK2/mitogen-activated protein kinase through interactions with and activation of c-Src. J Biol Chem 272, 13189-95
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87

Schlaepfer DD, Hunter T (1996) Evidence for in vivo phosphorylation of the Grb2 SH2-domain binding site on focal adhesion kinase by Src-family protein-tyrosine kinases. Mol Cell Biol 16, 5623-33
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88

Calalb MB, Polte TR, Hanks SK (1995) Tyrosine phosphorylation of focal adhesion kinase at sites in the catalytic domain regulates kinase activity: a role for Src family kinases. Mol Cell Biol 15, 954-63
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