Tyr925
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Home > Phosphorylation Site Page: > Tyr925  -  FAK (mouse)

Site Information
DRSNDKVyENVTGLV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447966
Associated spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 10 ) , immunoprecipitation ( 10 ) , mass spectrometry ( 4 ) , mutation of modification site ( 5 , 6 , 8 , 9 , 10 ) , phospho-antibody ( 1 , 2 , 3 , 5 , 6 , 8 , 9 ) , phosphoamino acid analysis ( 10 ) , phosphopeptide mapping ( 10 ) , western blotting ( 1 , 2 , 3 , 5 , 6 , 8 , 9 )
Disease tissue studied:
breast cancer ( 5 ) , liver cancer ( 6 ) , cholangiocellular carcinoma ( 6 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 9 , 10 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 8 , 10 ) , 4T1 (breast cell) ( 5 ) , E14 ('neuron, hippocampal') ( 2 ) , HEK293T (epithelial) ( 8 ) , MEF (fibroblast) ( 4 , 5 , 6 ) , MEF (fibroblast) [FAK iso2 (mouse), transgenic] ( 3 ) , MEF (fibroblast) [PTP1B (mouse), homozygous knockout] ( 4 ) , MEF-3T3 ( 1 )

Upstream Regulation
Regulatory protein:
FAK iso2 (mouse) ( 3 ) , FAK iso9 (mouse) ( 5 ) , PXN (mouse) ( 1 )
Putative in vivo kinases:
Src (human) ( 10 )
Kinases, in vitro:
Src (human) ( 10 , 11 )
Treatments:
calphostin_C ( 8 ) , cytochalasin_D ( 8 ) , fibronectin ( 3 , 8 , 9 , 10 ) , genistein ( 8 ) , herbimycin_A ( 8 ) , phorbol_ester ( 8 ) , poly-L-lysine ( 8 ) , serum_starvation ( 8 ) , TCAP-1 ( 2 ) , TNF ( 6 ) , tyrphostin_A47 ( 8 )

Downstream Regulation
Effects of modification on FAK:
molecular association, regulation ( 6 , 9 , 10 , 11 ) , phosphorylation ( 5 , 8 )
Effects of modification on biological processes:
cell growth, altered ( 5 ) , transcription, altered ( 6 )
Induce interaction with:
GRB2 (human) ( 9 , 10 ) , GRB2 (mouse) ( 6 , 11 )

References 

1

Quizi JL, et al. (2013) SLK-mediated phosphorylation of paxillin is required for focal adhesion turnover and cell migration. Oncogene 32, 4656-63
23128389   Curated Info

2

Chand D, et al. (2012) C-Terminal region of teneurin-1 co-localizes with dystroglycan and modulates cytoskeletal organization through an extracellular signal-regulated kinase-dependent stathmin- and filamin A-mediated mechanism in hippocampal cells. Neuroscience 219, 255-70
22698694   Curated Info

3

Corsi JM, et al. (2009) Autophosphorylation-independent and -dependent functions of focal adhesion kinase during development. J Biol Chem 284, 34769-76
19776009   Curated Info

4

Mertins P, et al. (2008) Investigation of protein-tyrosine phosphatase 1B function by quantitative proteomics. Mol Cell Proteomics 7, 1763-77
18515860   Curated Info

5

Mitra SK, et al. (2006) Intrinsic FAK activity and Y925 phosphorylation facilitate an angiogenic switch in tumors. Oncogene 25, 5969-84
16682956   Curated Info

6

Mon NN, et al. (2006) A role for focal adhesion kinase signaling in tumor necrosis factor-alpha-dependent matrix metalloproteinase-9 production in a cholangiocarcinoma cell line, CCKS1. Cancer Res 66, 6778-84
16818654   Curated Info

7

Nakamura K, Yano H, Schaefer E, Sabe H (2001) Different modes and qualities of tyrosine phosphorylation of Fak and Pyk2 during epithelial-mesenchymal transdifferentiation and cell migration: analysis of specific phosphorylation events using site-directed antibodies. Oncogene 20, 2626-35
11420674   Curated Info

8

Schlaepfer DD, Jones KC, Hunter T (1998) Multiple Grb2-mediated integrin-stimulated signaling pathways to ERK2/mitogen-activated protein kinase: summation of both c-Src- and focal adhesion kinase-initiated tyrosine phosphorylation events. Mol Cell Biol 18, 2571-85
9566877   Curated Info

9

Schlaepfer DD, Hunter T (1997) Focal adhesion kinase overexpression enhances ras-dependent integrin signaling to ERK2/mitogen-activated protein kinase through interactions with and activation of c-Src. J Biol Chem 272, 13189-95
9148935   Curated Info

10

Schlaepfer DD, Hunter T (1996) Evidence for in vivo phosphorylation of the Grb2 SH2-domain binding site on focal adhesion kinase by Src-family protein-tyrosine kinases. Mol Cell Biol 16, 5623-33
8816475   Curated Info

11

Schlaepfer DD, Hanks SK, Hunter T, van der Geer P (1994) Integrin-mediated signal transduction linked to Ras pathway by GRB2 binding to focal adhesion kinase. Nature 372, 786-91
7997267   Curated Info