Ser766
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Home > Phosphorylation Site Page: > Ser766  -  LARP (human)

Site Information
EPStIARsLPttVPE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 483150

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 20 , 21 , 22 , 23 , 24 , 25 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 )
Disease tissue studied:
breast cancer ( 10 , 11 , 22 , 23 ) , breast ductal carcinoma ( 10 ) , HER2 positive breast cancer ( 2 ) , luminal A breast cancer ( 2 ) , luminal B breast cancer ( 2 ) , breast cancer, surrounding tissue ( 2 ) , breast cancer, triple negative ( 2 , 10 ) , cervical cancer ( 42 ) , cervical adenocarcinoma ( 42 ) , gastric cancer ( 32 ) , gastric carcinoma ( 32 ) , leukemia ( 27 , 52 ) , acute myelogenous leukemia ( 27 ) , chronic myelogenous leukemia ( 52 ) , lung cancer ( 7 , 16 , 23 , 31 , 35 ) , non-small cell lung cancer ( 23 ) , non-small cell lung adenocarcinoma ( 7 , 16 , 31 ) , lymphoma ( 12 ) , Burkitt's lymphoma ( 12 ) , follicular lymphoma ( 12 ) , mantle cell lymphoma ( 12 ) , neuroblastoma ( 21 ) , ovarian cancer ( 10 ) , pancreatic ductal adenocarcinoma ( 15 ) , multiple myeloma ( 40 ) , prostate cancer ( 41 ) , melanoma skin cancer ( 6 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 28 ) , 'pancreatic, ductal'-pancreas ( 15 ) , 293 (epithelial) [AT1 (human), transfection, AT1R stable transfected HEK293] ( 37 ) , 293 (epithelial) [AT1 (human), transfection] ( 36 ) , 293 (epithelial) ( 9 , 46 ) , 293E (epithelial) ( 30 ) , 786-O (renal) [VHL (human), transfection] ( 4 ) , 786-O (renal) ( 4 ) , A498 (renal) ( 39 ) , A549 (pulmonary) ( 17 ) , B lymphocyte-blood ( 40 ) , BJAB (B lymphocyte) ( 12 ) , breast ( 2 , 10 ) , BT-20 (breast cell) ( 23 ) , BT-549 (breast cell) ( 23 ) , Calu 6 (pulmonary) ( 23 ) , CL1-0 (pulmonary) ( 35 ) , CL1-1 (pulmonary) ( 35 ) , CL1-2 (pulmonary) ( 35 ) , CL1-5 (pulmonary) ( 35 ) , endothelial-aorta ( 24 ) , FL-318 (B lymphocyte) ( 12 ) , Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] ( 25 ) , Flp-In T-Rex-293 (epithelial) ( 25 ) , GM00130 (B lymphocyte) ( 38 ) , H2009 (pulmonary) ( 23 ) , H2077 (pulmonary) ( 23 ) , H2887 (pulmonary) ( 23 ) , H322M (pulmonary) ( 23 ) , HCC1359 (pulmonary) ( 23 ) , HCC1937 (breast cell) ( 23 ) , HCC2279 (pulmonary) ( 23 ) , HCC366 (pulmonary) ( 23 ) , HCC4006 (pulmonary) ( 23 ) , HCC78 (pulmonary) ( 23 ) , HCC827 (pulmonary) ( 23 ) , HCT116 (intestinal) ( 45 ) , HEK293T (epithelial) ( 5 ) , HeLa (cervical) ( 1 , 8 , 20 , 29 , 34 , 43 , 48 , 49 , 50 , 53 , 54 , 55 ) , HeLa S3 (cervical) ( 42 ) , HMLER ('stem, breast cancer') [CXCR4 (human), knockdown] ( 11 ) , HMLER ('stem, breast cancer') ( 11 ) , HOP62 (pulmonary) ( 23 ) , HUES-7 ('stem, embryonic') ( 44 ) , HUES-9 ('stem, embryonic') ( 33 ) , JEKO-1 (B lymphocyte) ( 12 ) , Jurkat (T lymphocyte) ( 18 ) , K562 (erythroid) ( 20 , 43 , 51 , 52 ) , KG-1 (myeloid) ( 27 ) , LCLC-103H (pulmonary) ( 23 ) , liver ( 14 ) , LNCaP (prostate cell) ( 41 ) , LOU-NH91 (squamous) ( 23 ) , lung ( 16 ) , MCF-7 (breast cell) ( 23 ) , MDA-MB-231 (breast cell) ( 23 ) , MDA-MB-468 (breast cell) ( 23 ) , MKN-45 (gastric) ( 32 ) , NB10 (neural crest) ( 21 ) , NCEB-1 (B lymphocyte) ( 12 ) , NCI-H1395 (pulmonary) ( 23 ) , NCI-H1568 (pulmonary) ( 23 ) , NCI-H157 (pulmonary) ( 23 ) , NCI-H1648 (pulmonary) ( 23 ) , NCI-H1666 (pulmonary) ( 23 ) , NCI-H2030 (pulmonary) ( 23 ) , NCI-H2172 (pulmonary) ( 23 ) , NCI-H322 (pulmonary) ( 23 ) , NCI-H460 (pulmonary) ( 23 , 47 ) , NCI-H520 (squamous) ( 23 ) , NCI-H647 (pulmonary) ( 23 ) , NPC (neural crest) ( 21 ) , ovary ( 10 ) , PC9 (pulmonary) ( 7 , 23 ) , Raji (B lymphocyte) ( 12 ) , RAMOS (B lymphocyte) ( 12 ) , REC-1 (B lymphocyte) ( 12 ) , SH-SY5Y (neural crest) [LRRK2 (human), transfection, over-expression of LRRK2(G2019S)] ( 13 ) , SH-SY5Y (neural crest) ( 13 ) , SKBr3 (breast cell) ( 22 ) , U-1810 (pulmonary) [EFNB3 (human), knockdown] ( 31 ) , U-1810 (pulmonary) ( 31 ) , UPN-1 (B lymphocyte) ( 12 ) , WM239A (melanocyte) ( 6 )

Upstream Regulation
Putative in vivo kinases:
mTOR (human) ( 30 )
Treatments:
angiotensin_2 ( 37 ) , LRRK2-IN-1 ( 13 ) , nocodazole ( 42 ) , Torin1 ( 30 )

References 

1

Huang H, et al. (2016) Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography. Mol Cell Proteomics 15, 3282-3296
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2

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
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3

Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610
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4

Malec V, Coulson JM, Urbé S, Clague MJ (2015) Combined Analyses of the VHL and Hypoxia Signaling Axes in an Isogenic Pairing of Renal Clear Cell Carcinoma Cells. J Proteome Res 14, 5263-72
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5

Franchin C, et al. (2015) Quantitative analysis of a phosphoproteome readily altered by the protein kinase CK2 inhibitor quinalizarin in HEK-293T cells. Biochim Biophys Acta 1854, 609-23
25278378   Curated Info

6

Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615
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7

Tsai CF, et al. (2015) Large-scale determination of absolute phosphorylation stoichiometries in human cells by motif-targeting quantitative proteomics. Nat Commun 6, 6622
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8

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
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9

Wang R, et al. (2014) Global discovery of high-NaCl-induced changes of protein phosphorylation. Am J Physiol Cell Physiol 307, C442-54
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10

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
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11

Yi T, et al. (2014) Quantitative phosphoproteomic analysis reveals system-wide signaling pathways downstream of SDF-1/CXCR4 in breast cancer stem cells. Proc Natl Acad Sci U S A 111, E2182-90
24782546   Curated Info

12

Rolland D, et al. (2014) Global phosphoproteomic profiling reveals distinct signatures in B-cell non-Hodgkin lymphomas. Am J Pathol 184, 1331-42
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13

Luerman GC, et al. (2014) Phosphoproteomic evaluation of pharmacological inhibition of leucine-rich repeat kinase 2 reveals significant off-target effects of LRRK-2-IN-1. J Neurochem 128, 561-76
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14

Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
24275569   Curated Info

15

Britton D, et al. (2014) Quantification of pancreatic cancer proteome and phosphorylome: indicates molecular events likely contributing to cancer and activity of drug targets. PLoS One 9, e90948
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16

Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96
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17

Kim JY, et al. (2013) Dissection of TBK1 signaling via phosphoproteomics in lung cancer cells. Proc Natl Acad Sci U S A 110, 12414-9
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18

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
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19

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
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20

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
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21

DeNardo BD, et al. (2013) Quantitative phosphoproteomic analysis identifies activation of the RET and IGF-1R/IR signaling pathways in neuroblastoma. PLoS One 8, e82513
24349301   Curated Info

22

Imami K, et al. (2012) Temporal profiling of lapatinib-suppressed phosphorylation signals in EGFR/HER2 pathways. Mol Cell Proteomics 11, 1741-57
22964224   Curated Info

23

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
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24

Verano-Braga T, et al. (2012) Time-resolved quantitative phosphoproteomics: new insights into Angiotensin-(1-7) signaling networks in human endothelial cells. J Proteome Res 11, 3370-81
22497526   Curated Info

25

Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70
22496350   Curated Info

26

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

27

Weber C, Schreiber TB, Daub H (2012) Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells. J Proteomics 75, 1343-56
22115753   Curated Info

28

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

29

Nishioka T, Nakayama M, Amano M, Kaibuchi K (2012) Proteomic screening for Rho-kinase substrates by combining kinase and phosphatase inhibitors with 14-3-3ζ affinity chromatography. Cell Struct Funct 37, 39-48
22251793   Curated Info

30

Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22
21659604   Curated Info

31

Ståhl S, et al. (2011) Phosphoproteomic profiling of NSCLC cells reveals that ephrin B3 regulates pro-survival signaling through Akt1-mediated phosphorylation of the EphA2 receptor. J Proteome Res 10, 2566-78
21413766   Curated Info

32

Guo A (2011) CST Curation Set: 11298; Year: 2011; Biosample/Treatment: cell line, MKN-45/untreated; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

33

Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3
21406692   Curated Info

34

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

35

Wang YT, et al. (2010) An informatics-assisted label-free quantitation strategy that depicts phosphoproteomic profiles in lung cancer cell invasion. J Proteome Res 9, 5582-97
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36

Xiao K, et al. (2010) Global phosphorylation analysis of beta-arrestin-mediated signaling downstream of a seven transmembrane receptor (7TMR). Proc Natl Acad Sci U S A 107, 15299-304
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37

Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53
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38

Bennetzen MV, et al. (2010) Site-specific phosphorylation dynamics of the nuclear proteome during the DNA damage response. Mol Cell Proteomics 9, 1314-23
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39

Schreiber TB, et al. (2010) An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. Mol Cell Proteomics 9, 1047-62
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40

Ge F, et al. (2010) Phosphoproteomic analysis of primary human multiple myeloma cells. J Proteomics 73, 1381-90
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41

Chen L, Giorgianni F, Beranova-Giorgianni S (2010) Characterization of the phosphoproteome in LNCaP prostate cancer cells by in-gel isoelectric focusing and tandem mass spectrometry. J Proteome Res 9, 174-8
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42

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
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43

Pan C, Olsen JV, Daub H, Mann M (2009) Global effects of kinase inhibitors on signaling networks revealed by quantitative phosphoproteomics. Mol Cell Proteomics 8, 2796-808
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44

Van Hoof D, et al. (2009) Phosphorylation dynamics during early differentiation of human embryonic stem cells. Cell Stem Cell 5, 214-26
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45

Oppermann FS, et al. (2009) Large-scale proteomics analysis of the human kinome. Mol Cell Proteomics 8, 1751-64
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46

Gauci S, et al. (2009) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem 81, 4493-501
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47

Nagano K, et al. (2009) Phosphoproteomic analysis of distinct tumor cell lines in response to nocodazole treatment. Proteomics 9, 2861-74
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48

Chen Y, et al. (2009) Combined integrin phosphoproteomic analyses and small interfering RNA--based functional screening identify key regulators for cancer cell adhesion and migration. Cancer Res 69, 3713-20
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49

Chen RQ, et al. (2009) CDC25B mediates rapamycin-induced oncogenic responses in cancer cells. Cancer Res 69, 2663-8
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50

Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7
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51

Stokes M (2008) CST Curation Set: 4605; Year: 2008; Biosample/Treatment: cell line, K562/untreated; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

52

Stokes M (2008) CST Curation Set: 4388; Year: 2008; Biosample/Treatment: cell line, K562/untreated; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

53

McNulty DE, Annan RS (2008) Hydrophilic interaction chromatography reduces the complexity of the phosphoproteome and improves global phosphopeptide isolation and detection. Mol Cell Proteomics 7, 971-80
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54

Cantin GT, et al. (2008) Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis. J Proteome Res 7, 1346-51
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55

Beausoleil SA, et al. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization. Nat Biotechnol 24, 1285-92
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