Thr2511
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Home > Phosphorylation Site Page: > Thr2511  -  Notch 1 (human)

Site Information
VPEHPFLtPsPEsPD   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 483083

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 4 ) , electrophoretic mobility shift ( 1 ) , immunoprecipitation ( 1 , 2 , 3 , 4 ) , mass spectrometry ( 4 ) , microscopy-colocalization with upstream kinase ( 1 ) , mutation of modification site ( 1 , 2 , 3 , 4 ) , phospho-antibody ( 3 , 4 ) , western blotting ( 1 , 2 , 3 , 4 )
Disease tissue studied:
breast cancer ( 1 , 3 ) , breast adenocarcinoma ( 1 ) , breast cancer, triple negative ( 1 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 2 , 3 ) , CHO (fibroblast) ( 1 ) , HEK293T (epithelial) ( 1 ) , MCF-7 (breast cell) ( 3 ) , MDA-MB-231 (breast cell) ( 1 , 3 ) , MDA-MB-468 (breast cell) ( 1 ) , MEF (fibroblast) ( 2 , 4 ) , SF9 ( 4 ) , T-ALL (T lymphocyte) ( 4 ) , thymocyte ( 4 )

Upstream Regulation
Putative in vivo kinases:
CDK19 (human) ( 4 ) , CDK3 (human) ( 4 ) , CDK8 (human) ( 4 ) , DYRK2 (human) ( 1 ) , HIPK2 (human) ( 3 ) , MEKK1 (human) ( 2 )
Kinases, in vitro:
CDK19 (human) ( 4 ) , CDK3 (human) ( 4 ) , CDK8 (human) ( 4 ) , DYRK2 (human) ( 1 )
Treatments:
1NM-PP1 ( 1 ) , adriamycin ( 1 , 3 ) , CPT ( 1 ) , curcumin ( 1 ) , etoposide ( 1 ) , harmine ( 1 ) , siRNA ( 1 )

Downstream Regulation
Effects of modification on Notch 1:
activity, inhibited ( 1 ) , molecular association, regulation ( 4 ) , protein degradation ( 1 , 2 , 3 , 4 ) , ubiquitination ( 1 , 2 , 3 , 4 )
Effects of modification on biological processes:
carcinogenesis, altered ( 1 ) , carcinogenesis, inhibited ( 4 ) , cell growth, inhibited ( 3 ) , cell motility, altered ( 1 ) , cell motility, inhibited ( 3 ) , signaling pathway regulation ( 1 ) , transcription, inhibited ( 1 )
Induce interaction with:
FBXW7 (human) ( 4 )

Disease / Diagnostics Relevance
Relevant diseases:
breast cancer ( 3 )

References 

1

Morrugares R, et al. (2019) Phosphorylation-dependent regulation of the NOTCH1 intracellular domain by dual-specificity tyrosine-regulated kinase 2. Cell Mol Life Sci
31605148   Curated Info

2

Ahn JS, et al. (2016) Autophagy negatively regulates tumor cell proliferation through phosphorylation dependent degradation of the Notch1 intracellular domain. Oncotarget 7, 79047-79063
27806347   Curated Info

3

Ann EJ, et al. (2016) Tumor Suppressor HIPK2 Regulates Malignant Growth via Phosphorylation of Notch1. Cancer Res 76, 4728-40
27335110   Curated Info

4

Li N, et al. (2014) Cyclin C is a haploinsufficient tumour suppressor. Nat Cell Biol 16, 1080-91
25344755   Curated Info