Ser63
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Home > Phosphorylation Site Page: > Ser63  -  ATF-1 (mouse)

Site Information
GILARRPsYRKILKD   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 449762

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ) , mutation of modification site ( 1 , 2 ) , phospho-antibody ( 1 , 2 , 16 , 17 , 18 ) , western blotting ( 1 , 2 , 17 , 18 )
Relevant cell line - cell type - tissue:
'brain, embryonic' ( 14 , 15 ) , 293 (epithelial) ( 1 , 2 ) , 3T3-L1 (fibroblast) ( 8 , 9 ) , brain ( 13 ) , D5 (T lymphocyte) ( 18 ) , fibroblast-lung ( 4 , 5 , 6 , 7 ) , heart ( 12 ) , HeLa (cervical) ( 1 ) , liver ( 10 , 11 ) , macrophage-bone marrow ( 2 ) , MEF (fibroblast) ( 1 ) , MEF (fibroblast) [MSK1 (mouse), homozygous knockout, msk2] ( 17 ) , RAW 264.7 (macrophage) ( 2 ) , STC-1 (endocrine) ( 16 )

Upstream Regulation
Regulatory protein:
MSK1 (human) ( 2 ) , MSK1 (mouse) ( 17 ) , MSK2 (mouse) ( 17 )
Treatments:
anisomycin ( 17 ) , BAPTA-AM ( 16 ) , butyrate ( 18 ) , cAMP_analog ( 2 ) , colforsin ( 1 , 17 , 18 ) , EGF ( 17 ) , H-89 ( 16 , 17 ) , heat_shock ( 1 ) , IBMX ( 17 ) , LPS ( 2 ) , MG132 ( 1 ) , PD184352 ( 17 ) , peptones ( 16 ) , PGE2 ( 2 ) , phorbol_ester ( 17 ) , Ro31-8220 ( 17 ) , SB203580 ( 17 ) , trichostatin_A ( 18 ) , UV ( 17 )

Downstream Regulation
Effects of modification on ATF-1:
acetylation ( 1 ) , molecular association, regulation ( 1 )
Effects of modification on biological processes:
cell growth, inhibited ( 1 )
Inhibit interaction with:
DNA ( 1 )

References 

1

Takii R, et al. (2015) ATF1 Modulates the Heat Shock Response by Regulating the Stress-Inducible Heat Shock Factor 1 Transcription Complex. Mol Cell Biol 35, 11-25
25312646   Curated Info

2

Mackenzie KF, et al. (2013) PGE2 Induces Macrophage IL-10 Production and a Regulatory-like Phenotype via a Protein Kinase A-SIK-CRTC3 Pathway. J Immunol 190, 565-77
23241891   Curated Info

3

Dickinson RJ, et al. (2011) Phosphorylation of the kinase interaction motif in mitogen-activated protein (MAP) kinase phosphatase-4 mediates cross-talk between protein kinase A and MAP kinase signaling pathways. J Biol Chem 286, 38018-26
21908610   Curated Info

4

Guo A (2009) CST Curation Set: 6378; Year: 2009; Biosample/Treatment: lung, fibroblast/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

5

Guo A (2009) CST Curation Set: 6375; Year: 2009; Biosample/Treatment: lung, fibroblast/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

6

Guo A (2009) CST Curation Set: 6376; Year: 2009; Biosample/Treatment: lung, fibroblast/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

7

Guo A (2009) CST Curation Set: 6374; Year: 2009; Biosample/Treatment: lung, fibroblast/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

8

Possemato A (2008) CST Curation Set: 4894; Year: 2008; Biosample/Treatment: cell line, 3T3-L1/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

9

Possemato A (2008) CST Curation Set: 4895; Year: 2008; Biosample/Treatment: cell line, 3T3-L1/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

10

Possemato A (2008) CST Curation Set: 3855; Year: 2008; Biosample/Treatment: tissue, liver/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986
Curated Info

11

Possemato A (2008) CST Curation Set: 3856; Year: 2008; Biosample/Treatment: tissue, liver/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKA Substrate Antibody Cat#: 9621
Curated Info

12

Possemato A (2008) CST Curation Set: 3846; Year: 2008; Biosample/Treatment: tissue, heart/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986
Curated Info

13

Possemato A (2008) CST Curation Set: 3852; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKA Substrate Antibody Cat#: 9621
Curated Info

14

Possemato A (2008) CST Curation Set: 3826; Year: 2008; Biosample/Treatment: tissue, brain, embryonic/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986
Curated Info

15

Possemato A (2008) CST Curation Set: 3827; Year: 2008; Biosample/Treatment: tissue, brain, embryonic/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKA Substrate Antibody Cat#: 9621
Curated Info

16

Gevrey JC, et al. (2002) Co-requirement of cyclic AMP- and calcium-dependent protein kinases for transcriptional activation of cholecystokinin gene by protein hydrolysates. J Biol Chem 277, 22407-13
11950843   Curated Info

17

Wiggin GR, et al. (2002) MSK1 and MSK2 are required for the mitogen- and stress-induced phosphorylation of CREB and ATF1 in fibroblasts. Mol Cell Biol 22, 2871-81
11909979   Curated Info

18

Michael LF, et al. (2000) The phosphorylation status of a cyclic AMP-responsive activator is modulated via a chromatin-dependent mechanism. Mol Cell Biol 20, 1596-603
10669737   Curated Info