Thr57
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Home > Phosphorylation Site Page: > Thr57  -  EEF2 (rat)

Site Information
RAGEtRFtDtRKDEQ   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448040

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 3 , 4 , 6 ) , phospho-antibody ( 1 , 2 , 5 , 7 ) , western blotting ( 1 , 2 , 5 )
Relevant cell line - cell type - tissue:
'heart, artery' ( 1 ) , 'muscle, skeletal' ( 5 ) , 'neuron, hippocampal, CA1 pyramidal'-brain ( 2 ) , adipose tissue ( 3 ) , heart ( 3 ) , hepatocyte-liver ( 7 ) , intestine ( 3 ) , kidney ( 3 ) , liver ( 3 , 4 , 6 ) , lung ( 3 ) , muscle ( 3 ) , pancreas ( 3 ) , spleen ( 3 ) , stomach ( 3 ) , testis ( 3 ) , thymus ( 3 )

Upstream Regulation
Putative in vivo kinases:
EEF2K (rat) ( 1 , 7 , 8 )
Kinases, in vitro:
EEF2K (rat) ( 8 )
Treatments:
2-deoxyglucose ( 7 ) , acadesine ( 7 ) , anoxia ( 7 ) , development ( 1 , 5 ) , insulin ( 7 ) , mechanical stress ( 5 ) , novel object recognition test ( 2 ) , oligomycin ( 7 ) , rapamycin ( 7 ) , stress ( 2 )

Downstream Regulation
Effects of modification on EEF2:
activity, inhibited ( 7 )

Disease / Diagnostics Relevance
Relevant diseases:
hypertension ( 1 )

References 

1

Kodama T, Otani K, Okada M, Yamawaki H (2020) Age-dependent increase in activity of eukaryotic elongation factor 2 kinase in mesenteric arteries from spontaneously hypertensive rats. J Vet Med Sci
33191388   Curated Info

2

Calabrese F, et al. (2017) Chronic Mild Stress-Induced Alterations of Local Protein Synthesis: A Role for Cognitive Impairment. ACS Chem Neurosci 8, 817-825
28094500   Curated Info

3

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

4

Demirkan G, et al. (2011) Phosphoproteomic Profiling of In Vivo Signaling in Liver by the Mammalian Target of Rapamycin Complex 1 (mTORC1). PLoS One 6, e21729
21738781   Curated Info

5

Thomson DM, Gordon SE (2006) Impaired overload-induced muscle growth is associated with diminished translational signalling in aged rat fast-twitch skeletal muscle. J Physiol 574, 291-305
16627569   Curated Info

6

Moser K, White FM (2006) Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS. J Proteome Res 5, 98-104
16396499   Curated Info

7

Horman S, et al. (2002) Activation of AMP-activated protein kinase leads to the phosphorylation of elongation factor 2 and an inhibition of protein synthesis. Curr Biol 12, 1419-23
12194824   Curated Info

8

Nairn AC, Palfrey HC (1987) Identification of the major Mr 100,000 substrate for calmodulin-dependent protein kinase III in mammalian cells as elongation factor-2. J Biol Chem 262, 17299-303
3693353   Curated Info