Tyr527
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Home > Phosphorylation Site Page: > Tyr527  -  Src (chicken)

Site Information
FTSTEPQyQPGENL_   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448235

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 10 , 15 , 16 ) , [32P] bio-synthetic labeling ( 9 , 11 , 12 , 16 ) , immunoprecipitation ( 14 , 15 ) , mutation of modification site ( 2 , 3 , 5 , 8 , 9 , 10 , 11 , 13 , 14 , 15 ) , peptide sequencing ( 12 ) , phospho-antibody ( 2 , 3 , 4 , 6 ) , phosphoamino acid analysis ( 16 ) , phosphopeptide mapping ( 9 , 10 , 11 , 14 , 15 ) , western blotting ( 2 , 3 , 10 , 13 , 14 , 15 )
Relevant cell line - cell type - tissue:
3T3 (fibroblast) ( 3 , 10 , 14 , 15 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 6 , 11 , 16 ) , 3Y1 (fibroblast) ( 8 ) , CEF ( 4 ) , CHO (fibroblast) ( 2 ) , MEF (fibroblast) ( 12 ) , SYF (fibroblast) ( 5 ) , yeast cell ( 9 )

Upstream Regulation
Regulatory protein:
PTPRA (human) ( 3 ) , PTPRA (mouse) ( 3 ) , Src (chicken) ( 3 )
Putative in vivo kinases:
CSK (human) ( 9 , 13 )
Kinases, in vitro:
CSK (human) ( 9 )
Putative upstream phosphatases:
PTPRA (human) ( 6 )
Phosphatases, in vitro:
PTPRA (human) ( 6 , 7 )
Treatments:
cell_adhesion ( 3 ) , fibrinogen ( 2 )

Downstream Regulation
Effects of modification on Src:
enzymatic activity, inhibited ( 3 , 5 , 6 , 10 , 11 , 15 , 16 ) , molecular association, regulation ( 1 , 2 ) , phosphorylation ( 14 )
Effects of modification on biological processes:
cell adhesion, altered ( 2 , 3 ) , cell growth, altered ( 2 , 9 , 14 , 15 ) , cell motility, altered ( 3 ) , cytoskeletal reorganization ( 2 )
Induce interaction with:
CSK (rat) ( 2 )
Inhibit interaction with:
CSK (human) ( 1 )

References 

1

Levinson NM, Seeliger MA, Cole PA, Kuriyan J (2008) Structural basis for the recognition of c-Src by its inactivator Csk. Cell 134, 124-34
18614016   Curated Info

2

Vielreicher M, et al. (2007) Dynamic interaction between Src and C-terminal Src kinase in integrin alphaIIbbeta3-mediated signaling to the cytoskeleton. J Biol Chem 282, 33623-31
17855352   Curated Info

3

Giannoni E, et al. (2005) Intracellular reactive oxygen species activate Src tyrosine kinase during cell adhesion and anchorage-dependent cell growth. Mol Cell Biol 25, 6391-403
16024778   Curated Info

4

Akagi T, Murata K, Shishido T, Hanafusa H (2002) v-Crk activates the phosphoinositide 3-kinase/AKT pathway by utilizing focal adhesion kinase and H-Ras. Mol Cell Biol 22, 7015-23
12242282   Curated Info

5

Cary LA, Klinghoffer RA, Sachsenmaier C, Cooper JA (2002) SRC catalytic but not scaffolding function is needed for integrin-regulated tyrosine phosphorylation, cell migration, and cell spreading. Mol Cell Biol 22, 2427-40
11909938   Curated Info

6

Zheng XM, Shalloway D (2001) Two mechanisms activate PTPalpha during mitosis. EMBO J 20, 6037-49
11689444   Curated Info

7

Zheng XM, Resnick RJ, Shalloway D (2000) A phosphotyrosine displacement mechanism for activation of Src by PTPalpha. EMBO J 19, 964-78
10698938   Curated Info

8

Zang Q, et al. (1997) Association between v-Src and protein kinase C delta in v-Src-transformed fibroblasts. J Biol Chem 272, 13275-80
9148947   Curated Info

9

Superti-Furga G, et al. (1993) Csk inhibition of c-Src activity requires both the SH2 and SH3 domains of Src. EMBO J 12, 2625-34
7687537   Curated Info

10

Bagrodia S, Taylor SJ, Shalloway D (1993) Myristylation is required for Tyr-527 dephosphorylation and activation of pp60c-src in mitosis. Mol Cell Biol 13, 1464-70
7680096   Curated Info

11

Shenoy S, et al. (1992) Role of p34cdc2-mediated phosphorylations in two-step activation of pp60c-src during mitosis. Proc Natl Acad Sci U S A 89, 7237-41
1379736   Curated Info

12

Thomas JE, Soriano P, Brugge JS (1991) Phosphorylation of c-Src on tyrosine 527 by another protein tyrosine kinase. Science 254, 568-71
1719633   Curated Info

13

Nada S, et al. (1991) Cloning of a complementary DNA for a protein-tyrosine kinase that specifically phosphorylates a negative regulatory site of p60c-src. Nature 351, 69-72
1709258   Curated Info

14

Yaciuk P, Choi JK, Shalloway D (1989) Mutation of amino acids in pp60c-src that are phosphorylated by protein kinases C and A. Mol Cell Biol 9, 2453-63
2474754   Curated Info

15

Piwnica-Worms H, et al. (1987) Tyrosine phosphorylation regulates the biochemical and biological properties of pp60c-src. Cell 49, 75-82
3103926   Curated Info

16

Kmiecik TE, Shalloway D (1987) Activation and suppression of pp60c-src transforming ability by mutation of its primary sites of tyrosine phosphorylation. Cell 49, 65-73
3103925   Curated Info