Tyr416
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Home > Phosphorylation Site Page: > Tyr416  -  Src (chicken)

Site Information
RLIEDNEyTARQGAK   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448234

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 11 , 12 , 13 ) , [32P] bio-synthetic labeling ( 8 , 10 , 12 , 13 ) , immunoprecipitation ( 9 , 11 , 13 ) , mutation of modification site ( 2 , 5 , 7 , 8 , 9 , 10 , 11 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 6 , 7 ) , phosphoamino acid analysis ( 12 ) , phosphopeptide mapping ( 8 , 9 , 10 , 11 , 13 ) , western blotting ( 1 , 2 , 3 , 9 , 11 )
Relevant cell line - cell type - tissue:
'retina, embryo' ( 4 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 10 , 12 ) , 3T3 (fibroblast) ( 3 , 9 , 11 ) , CEF ( 6 ) , CHO (fibroblast) ( 2 ) , DF1 (fibroblast) ( 1 ) , fibroblast-embryo ( 13 ) , HEK293T (epithelial) ( 1 ) , SYF (fibroblast) ( 7 ) , yeast cell ( 8 )

Upstream Regulation
Regulatory protein:
CSK (human) ( 8 ) , DAB1 (chicken) ( 4 ) , ITGA4 (human) ( 1 ) , PTPRA (human) ( 3 ) , Src (chicken) ( 3 ) , Src (human) ( 3 )
Kinases, in vitro:
Src (chicken) ( 8 )
Treatments:
AP1510 ( 5 ) , catalase ( 3 ) , cell_adhesion ( 3 ) , fibrinogen ( 2 ) , glucose_oxidase ( 3 ) , masoprocol ( 3 ) , saracatinib ( 1 ) , virus infection ( 1 )

Downstream Regulation
Effects of modification on Src:
enzymatic activity, induced ( 3 , 5 , 7 , 11 , 12 ) , phosphorylation ( 9 )
Effects of modification on biological processes:
cell adhesion, altered ( 3 ) , cell growth, altered ( 9 , 11 , 13 ) , cell motility, altered ( 3 ) , cytoskeletal reorganization ( 1 )

References 

1

Ye C, et al. (2017) Infectious Bursal Disease Virus Activates c-Src To Promote α4β1 Integrin-Dependent Viral Entry by Modulating the Downstream Akt-RhoA GTPase-Actin Rearrangement Cascade. J Virol 91
27881656   Curated Info

2

Vielreicher M, et al. (2007) Dynamic interaction between Src and C-terminal Src kinase in integrin alphaIIbbeta3-mediated signaling to the cytoskeleton. J Biol Chem 282, 33623-31
17855352   Curated Info

3

Giannoni E, et al. (2005) Intracellular reactive oxygen species activate Src tyrosine kinase during cell adhesion and anchorage-dependent cell growth. Mol Cell Biol 25, 6391-403
16024778   Curated Info

4

Katyal S, Godbout R (2004) Alternative splicing modulates Disabled-1 (Dab1) function in the developing chick retina. EMBO J 23, 1878-88
15057276   Curated Info

5

Arias-Salgado EG, et al. (2003) Src kinase activation by direct interaction with the integrin beta cytoplasmic domain. Proc Natl Acad Sci U S A 100, 13298-302
14593208   Curated Info

6

Akagi T, Murata K, Shishido T, Hanafusa H (2002) v-Crk activates the phosphoinositide 3-kinase/AKT pathway by utilizing focal adhesion kinase and H-Ras. Mol Cell Biol 22, 7015-23
12242282   Curated Info

7

Cary LA, Klinghoffer RA, Sachsenmaier C, Cooper JA (2002) SRC catalytic but not scaffolding function is needed for integrin-regulated tyrosine phosphorylation, cell migration, and cell spreading. Mol Cell Biol 22, 2427-40
11909938   Curated Info

8

Superti-Furga G, et al. (1993) Csk inhibition of c-Src activity requires both the SH2 and SH3 domains of Src. EMBO J 12, 2625-34
7687537   Curated Info

9

Yaciuk P, Choi JK, Shalloway D (1989) Mutation of amino acids in pp60c-src that are phosphorylated by protein kinases C and A. Mol Cell Biol 9, 2453-63
2474754   Curated Info

10

Kmiecik TE, Johnson PJ, Shalloway D (1988) Regulation by the autophosphorylation site in overexpressed pp60c-src. Mol Cell Biol 8, 4541-6
2460746   Curated Info

11

Piwnica-Worms H, et al. (1987) Tyrosine phosphorylation regulates the biochemical and biological properties of pp60c-src. Cell 49, 75-82
3103926   Curated Info

12

Kmiecik TE, Shalloway D (1987) Activation and suppression of pp60c-src transforming ability by mutation of its primary sites of tyrosine phosphorylation. Cell 49, 65-73
3103925   Curated Info

13

Iba H, Cross FR, Garber EA, Hanafusa H (1985) Low level of cellular protein phosphorylation by nontransforming overproduced p60c-src. Mol Cell Biol 5, 1058-66
2582236   Curated Info