Ser59

Site Information
PSFLRAPsWIDtGLS SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 448783

In vivo Characterization
Methods used to characterize site in vivo:	immunoassay ( 2 ) , mass spectrometry ( 3 , 4 , 5 , 6 , 8 , 10 ) , mutation of modification site ( 1 , 12 ) , phospho-antibody ( 1 , 2 , 7 , 9 , 11 , 12 , 13 , 14 , 15 ) , western blotting ( 1 , 7 , 9 , 11 , 12 , 15 )
Relevant cell line - cell type - tissue:	'fat, brown' ( 6 ) , 32Dcl3 (myeloid) [FLT3 (mouse), transfection, chimera with human FLT3-ITD mutant (corresponding to wild type P36888 ( 10 ) , 32Dcl3 (myeloid) ( 10 ) , astrocyte ( 2 ) , brain ( 2 , 8 ) , C2C12 (myoblast) ( 13 ) , heart ( 5 , 6 , 12 ) , heart [PKN1 (mouse), transgenic] ( 7 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 3 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 3 ) , HL-1 (myocyte) ( 1 , 3 ) , kidney ( 6 ) , lung ( 6 ) , myocyte-heart ( 11 , 12 , 14 , 15 ) , myocyte-heart [PKN1 (mouse), transgenic] ( 7 ) , retina ( 9 ) , stromal ( 4 )

In vivo Characterization

Methods used to characterize site in vivo:

immunoassay ( 2 ) , mass spectrometry ( 3 , 4 , 5 , 6 , 8 , 10 ) , mutation of modification site ( 1 , 12 ) , phospho-antibody ( 1 , 2 , 7 , 9 , 11 , 12 , 13 , 14 , 15 ) , western blotting ( 1 , 7 , 9 , 11 , 12 , 15 )

Relevant cell line - cell type - tissue:

'fat, brown' ( 6 ) , 32Dcl3 (myeloid) [FLT3 (mouse), transfection, chimera with human FLT3-ITD mutant (corresponding to wild type P36888 ( 10 ) , 32Dcl3 (myeloid) ( 10 ) , astrocyte ( 2 ) , brain ( 2 , 8 ) , C2C12 (myoblast) ( 13 ) , heart ( 5 , 6 , 12 ) , heart [PKN1 (mouse), transgenic] ( 7 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 3 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 3 ) , HL-1 (myocyte) ( 1 , 3 ) , kidney ( 6 ) , lung ( 6 ) , myocyte-heart ( 11 , 12 , 14 , 15 ) , myocyte-heart [PKN1 (mouse), transgenic] ( 7 ) , retina ( 9 ) , stromal ( 4 )

Upstream Regulation
Regulatory protein:	ADRB1 (mouse) ( 5 ) , MKK6 (human) ( 15 ) , PKN1 (mouse) ( 7 )
Putative in vivo kinases:	P38A (human) ( 2 , 11 , 13 ) , PKN1 (mouse) ( 7 )
Treatments:	arsenite ( 15 ) , bisindolylmaleimide ( 13 ) , cuprizone ( 2 ) , cytochalasin_D ( 13 ) , H-89 ( 13 ) , heat_shock ( 13 ) , hyperoxia ( 9 ) , ischemia/reperfusion ( 11 , 12 ) , LPS ( 2 ) , oxidative_stress ( 2 ) , PD98059 ( 15 ) , PDGF ( 14 ) , SB202190 ( 12 ) , SB203580 ( 2 , 13 , 14 , 15 ) , sorbitol ( 15 ) , vinblastine ( 13 ) , virus infection ( 1 ) , Y27632 ( 13 )

Downstream Regulation
Effects of modification on CRYAB:	intracellular localization ( 2 , 11 ) , molecular association, regulation ( 2 ) , protein degradation ( 1 )
Effects of modification on biological processes:	apoptosis, inhibited ( 15 ) , cytoskeletal reorganization ( 13 ) , signaling pathway regulation ( 2 )

References
1	Fung G, et al. (2017) Phosphorylation and degradation of αB-crystallin during enterovirus infection facilitates viral replication and induces viral pathogenesis. Oncotarget 8, 74767-74780 29088822 Curated Info
2	Kuipers HF, et al. (2017) Phosphorylation of αB-crystallin supports reactive astrogliosis in demyelination. Proc Natl Acad Sci U S A 114, E1745-E1754 28196893 Curated Info
3	Reinartz M, Raupach A, Kaisers W, Gödecke A (2014) AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes. J Proteome Res 13, 4232-45 25162660 Curated Info
4	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
5	Lundby A, et al. (2013) In vivo phosphoproteomics analysis reveals the cardiac targets of β-adrenergic receptor signaling. Sci Signal 6, rs11 23737553 Curated Info
6	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info
7	Takagi H, et al. (2010) Activation of PKN mediates survival of cardiac myocytes in the heart during ischemia/reperfusion. Circ Res 107, 642-9 20595653 Curated Info
8	Wiśniewski JR, et al. (2010) Brain phosphoproteome obtained by a FASP-based method reveals plasma membrane protein topology. J Proteome Res 9, 3280-9 20415495 Curated Info
9	Kase S, et al. (2010) alphaB-crystallin regulation of angiogenesis by modulation of VEGF. Blood 115, 3398-406 20023214 Curated Info
10	Choudhary C, et al. (2009) Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes. Mol Cell 36, 326-39 19854140 Curated Info
11	Whittaker R, et al. (2009) Kinetics of the translocation and phosphorylation of alphaB-crystallin in mouse heart mitochondria during ex vivo ischemia. Am J Physiol Heart Circ Physiol 296, H1633-42 19252088 Curated Info
12	Jin JK, et al. (2008) Localization of phosphorylated {alpha}B-crystallin to heart mitochondria during ischemia-reperfusion. Am J Physiol Heart Circ Physiol 294, H337-44 17993600 Curated Info
13	Launay N, et al. (2006) Cell signaling pathways to alphaB-crystallin following stresses of the cytoskeleton. Exp Cell Res 312, 3570-84 16979163 Curated Info
14	Shu E, et al. (2005) alphaB-crystallin is phosphorylated during myocardial infarction: involvement of platelet-derived growth factor-BB. Arch Biochem Biophys 438, 111-8 15907784 Curated Info
15	Hoover HE, Thuerauf DJ, Martindale JJ, Glembotski CC (2000) alpha B-crystallin gene induction and phosphorylation by MKK6-activated p38. A potential role for alpha B-crystallin as a target of the p38 branch of the cardiac stress response. J Biol Chem 275, 23825-33 10816593 Curated Info