Tyr31
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Home > Phosphorylation Site Page: > Tyr31  -  PXN (chicken)

Site Information
FLTEEtPySYPTGNH   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448785

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 ) , mutation of modification site ( 5 , 6 ) , phospho-antibody ( 3 , 5 , 6 ) , western blotting ( 3 , 5 , 6 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
TrkA (human) ( 5 )
Kinases, in vitro:
Src (chicken) ( 4 )
Treatments:
ACh ( 3 ) , angiotensin ( 4 ) , calyculin_A ( 1 ) , collagen_I ( 6 ) , fibronectin ( 4 ) , PDGF ( 4 ) , serum_starvation ( 4 ) , vanadate ( 1 )

Downstream Regulation
Effects of modification on PXN:
molecular association, regulation ( 3 , 5 )
Effects of modification on biological processes:
cytoskeletal reorganization ( 3 )
Induce interaction with:
CDK6 (human) ( 3 ) , CRK (chicken) ( 5 )

References 

1

Webb DJ, et al. (2005) Paxillin phosphorylation sites mapped by mass spectrometry. J Cell Sci 118, 4925-9
16254239   Curated Info

2

Schroeder MJ, et al. (2005) Methods for the detection of paxillin post-translational modifications and interacting proteins by mass spectrometry. J Proteome Res 4, 1832-41
16212439   Curated Info

3

Tang DD, Turner CE, Gunst SJ (2003) Expression of non-phosphorylatable paxillin mutants in canine tracheal smooth muscle inhibits tension development. J Physiol 553, 21-35
12949231   Curated Info

4

Schaller MD, Schaefer EM (2001) Multiple stimuli induce tyrosine phosphorylation of the Crk-binding sites of paxillin. Biochem J 360, 57-66
11695992   Curated Info

5

Escalante M, et al. (2000) Phosphorylation of c-Crk II on the negative regulatory Tyr222 mediates nerve growth factor-induced cell spreading and morphogenesis. J Biol Chem 275, 24787-97
10825157   Curated Info

6

Petit V, et al. (2000) Phosphorylation of tyrosine residues 31 and 118 on paxillin regulates cell migration through an association with CRK in NBT-II cells. J Cell Biol 148, 957-70
10704446   Curated Info