Tyr31

Site Information
FLsEEPPysyPTGNH SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 448785
Associated spectra: 1 CST

In vivo Characterization
Methods used to characterize site in vivo:	immunoprecipitation ( 5 , 18 , 19 ) , mass spectrometry ( 6 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 21 , 22 , 23 ) , microscopy-colocalization with upstream kinase ( 24 ) , mutation of modification site ( 1 , 18 , 19 , 20 , 24 ) , phospho-antibody ( 2 , 3 , 4 , 5 , 7 , 16 , 17 , 18 , 19 , 24 ) , western blotting ( 1 , 2 , 3 , 5 , 7 , 17 , 18 , 19 , 24 )
Disease tissue studied:	breast cancer ( 17 ) , lung cancer ( 9 ) , testicular cancer ( 6 , 20 )
Relevant cell line - cell type - tissue:	3T3 (fibroblast) ( 2 , 4 , 17 , 21 , 22 , 23 ) , BaF3 ('B lymphocyte, precursor') [PXN (mouse)] ( 18 ) , BaF3 ('B lymphocyte, precursor') ( 1 ) , C2C12-D (muscle cell) ( 10 , 11 , 12 , 13 ) , CHO (fibroblast) [EphB1 (human), transfection] ( 19 ) , colon ( 7 ) , COS (fibroblast) ( 17 ) , embryo ( 3 ) , F9 (testicular) ( 6 , 20 ) , lung [EGFR (mouse), transgenic] ( 9 ) , MDA-MB-435 (breast cell) ( 17 ) , MDCK (epithelial) ( 2 ) , MEF (fibroblast) ( 3 , 5 , 8 , 14 , 15 , 17 ) , NMuMG (epithelial) ( 24 ) , P19 (testicular) ( 19 ) , RAW 264 (macrophage) ( 16 )

Upstream Regulation
Regulatory protein:	FAK iso2 (mouse) ( 3 )
Putative in vivo kinases:	Pyk2 (mouse) ( 4 ) , Src (mouse) ( 19 )
Treatments:	blebbistatin ( 5 ) , cell_adhesion ( 17 ) , ephrin_B2 ( 19 ) , fibronectin ( 3 ) , IL-3 ( 18 ) , lactacystin ( 17 ) , LPS ( 16 ) , MG132 ( 17 ) , PF271 ( 5 ) , phorbol_ester ( 18 ) , PP2 ( 5 , 19 ) , vanadate ( 5 )

Downstream Regulation
Effects of modification on PXN:	activity, induced ( 24 ) , molecular association, regulation ( 5 , 24 ) , phosphorylation ( 20 )
Effects of modification on biological processes:	apoptosis, altered ( 20 ) , apoptosis, inhibited ( 1 ) , cell adhesion, altered ( 24 ) , cell adhesion, induced ( 5 ) , cell motility, altered ( 19 , 20 , 24 ) , cytoskeletal reorganization ( 24 )
Induce interaction with:	RASA1 (mouse) ( 24 ) , Vinculin (mouse) ( 5 )

References
1	Nah AS, Chay KO (2019) Roles of paxillin phosphorylation in IL-3 withdrawal-induced Ba/F3 cell apoptosis. Genes Genomics 30604146 Curated Info
2	Lee HH, et al. (2010) Src-dependent phosphorylation of ROCK participates in regulation of focal adhesion dynamics. J Cell Sci 123, 3368-77 20826462 Curated Info
3	Lim ST, et al. (2010) Knock-in mutation reveals an essential role for focal adhesion kinase activity in blood vessel morphogenesis and cell motility-polarity but not cell proliferation. J Biol Chem 285, 21526-36 20442405 Curated Info
4	Bonnette PC, et al. (2010) Phosphoproteomic characterization of PYK2 signaling pathways involved in osteogenesis. J Proteomics 73, 1306-20 20116462 Curated Info
5	Pasapera AM, et al. (2010) Myosin II activity regulates vinculin recruitment to focal adhesions through FAK-mediated paxillin phosphorylation. J Cell Biol 188, 877-90 20308429 Curated Info
6	Zhou J (2010) CST Curation Set: 9105; Year: 2010; Biosample/Treatment: cell line, F9/untreated; Disease: testicular cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-102) Cat#: 9416 Curated Info
7	Zhao Y, et al. (2010) Identification and functional characterization of paxillin as a target of protein tyrosine phosphatase receptor T. Proc Natl Acad Sci U S A 107, 2592-7 20133777 Curated Info
8	Luo W, et al. (2008) Global impact of oncogenic Src on a phosphotyrosine proteome. J Proteome Res 7, 3447-60 18563927 Curated Info
9	Ren H (2008) CST Curation Set: 4335; Year: 2008; Biosample/Treatment: cell line, EGFR_WT/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
10	Guo A (2007) CST Curation Set: 2782; Year: 2007; Biosample/Treatment: cell line, C2C12-D/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
11	Guo A (2007) CST Curation Set: 2783; Year: 2007; Biosample/Treatment: cell line, C2C12-D/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
12	Guo A (2007) CST Curation Set: 2564; Year: 2007; Biosample/Treatment: cell line, C2C12-D/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
13	Guo A (2007) CST Curation Set: 2565; Year: 2007; Biosample/Treatment: cell line, C2C12-D/Insulin; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
14	Li Y (2006) CST Curation Set: 2051; Year: 2006; Biosample/Treatment: cell line, MEF/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
15	Li Y (2006) CST Curation Set: 2052; Year: 2006; Biosample/Treatment: cell line, MEF/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
16	Cai X, Li M, Vrana J, Schaller MD (2006) Glycogen synthase kinase 3- and extracellular signal-regulated kinase-dependent phosphorylation of paxillin regulates cytoskeletal rearrangement. Mol Cell Biol 26, 2857-68 16537926 Curated Info
17	Holcomb M, Rufini A, Barilà D, Klemke RL (2006) Deregulation of proteasome function induces Abl-mediated cell death by uncoupling p130CAS and c-CrkII. J Biol Chem 281, 2430-40 16267043 Curated Info
18	Romanova LY, et al. (2004) Phosphorylation of paxillin tyrosines 31 and 118 controls polarization and motility of lymphoid cells and is PMA-sensitive. J Cell Sci 117, 3759-68 15252114 Curated Info
19	Vindis C, et al. (2004) EphB1-mediated cell migration requires the phosphorylation of paxillin at Tyr-31/Tyr-118. J Biol Chem 279, 27965-70 15107421 Curated Info
20	Subauste MC, et al. (2004) Vinculin modulation of paxillin-FAK interactions regulates ERK to control survival and motility. J Cell Biol 165, 371-81 15138291 Curated Info
21	Moritz A (2003) CST Curation Set: 154; Year: 2003; Biosample/Treatment: cell line, 3T3/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
22	Moritz A (2003) CST Curation Set: 157; Year: 2003; Biosample/Treatment: cell line, 3T3/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
23	Moritz A (2003) CST Curation Set: 158; Year: 2003; Biosample/Treatment: cell line, 3T3/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
24	Tsubouchi A, et al. (2002) Localized suppression of RhoA activity by Tyr31/118-phosphorylated paxillin in cell adhesion and migration. J Cell Biol 159, 673-83 12446743 Curated Info