Tyr118
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Home > Phosphorylation Site Page: > Tyr118  -  PXN (chicken)

Site Information
AsEEEHVysFPNKQK   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448604

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 8 ) , [32P] bio-synthetic labeling ( 8 ) , immunoprecipitation ( 1 ) , mass spectrometry ( 2 , 3 ) , mutation of modification site ( 5 , 6 ) , phospho-antibody ( 1 , 4 , 5 , 6 ) , western blotting ( 1 , 4 , 6 )
Relevant cell line - cell type - tissue:
'muscle, smooth' ( 1 ) , 293 (epithelial) ( 2 ) , 293 (epithelial) [PXN (chicken)] ( 3 ) , 293T (epithelial) ( 6 ) , A7r5 ('muscle, smooth') ( 5 ) , CEF ( 8 ) , embryonic ( 5 ) , GN4 (epithelial) ( 5 ) , trachea ( 4 )

Upstream Regulation
Regulatory protein:
TrkA (human) ( 6 ) , Vinculin (chicken) ( 1 )
Kinases, in vitro:
FAK (human) ( 8 )
Treatments:
ACh ( 1 , 4 ) , angiotensin ( 5 ) , calyculin_A ( 2 ) , fibronectin ( 5 ) , PDGF ( 5 ) , serum_starvation ( 5 ) , vanadate ( 2 )

Downstream Regulation
Effects of modification on PXN:
molecular association, regulation ( 4 )
Effects of modification on biological processes:
cytoskeletal reorganization ( 4 )
Induce interaction with:
CDK6 (human) ( 4 )

References 

1

Huang Y, Zhang W, Gunst SJ (2011) Activation of vinculin induced by cholinergic stimulation regulates contraction of tracheal smooth muscle tissue. J Biol Chem 286, 3630-44
21071443   Curated Info

2

Webb DJ, et al. (2005) Paxillin phosphorylation sites mapped by mass spectrometry. J Cell Sci 118, 4925-9
16254239   Curated Info

3

Schroeder MJ, et al. (2005) Methods for the detection of paxillin post-translational modifications and interacting proteins by mass spectrometry. J Proteome Res 4, 1832-41
16212439   Curated Info

4

Tang DD, Turner CE, Gunst SJ (2003) Expression of non-phosphorylatable paxillin mutants in canine tracheal smooth muscle inhibits tension development. J Physiol 553, 21-35
12949231   Curated Info

5

Schaller MD, Schaefer EM (2001) Multiple stimuli induce tyrosine phosphorylation of the Crk-binding sites of paxillin. Biochem J 360, 57-66
11695992   Curated Info

6

Escalante M, et al. (2000) Phosphorylation of c-Crk II on the negative regulatory Tyr222 mediates nerve growth factor-induced cell spreading and morphogenesis. J Biol Chem 275, 24787-97
10825157   Curated Info

7

Petit V, et al. (2000) Phosphorylation of tyrosine residues 31 and 118 on paxillin regulates cell migration through an association with CRK in NBT-II cells. J Cell Biol 148, 957-70
10704446   Curated Info

8

Bellis SL, Perrotta JA, Curtis MS, Turner CE (1997) Adhesion of fibroblasts to fibronectin stimulates both serine and tyrosine phosphorylation of paxillin. Biochem J 325 ( Pt 2), 375-81
9230116   Curated Info