Ser214
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Home > Phosphorylation Site Page: > Ser214  -  MAD1L1 (human)

Site Information
kIQELQAsQEARADH   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 482335

In vivo Characterization
Methods used to characterize site in vivo:
flow cytometry ( 3 ) , immunoassay ( 1 ) , immunoprecipitation ( 3 ) , mass spectrometry ( 2 , 5 , 6 , 7 ) , mutation of modification site ( 1 , 3 ) , phospho-antibody ( 1 , 3 ) , western blotting ( 1 , 3 )
Disease tissue studied:
colorectal cancer ( 1 ) , colorectal carcinoma ( 1 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
ATM (human) ( 1 , 3 )
Kinases, in vitro:
ATM (human) ( 3 )
Treatments:
ionizing_radiation ( 1 ) , siRNA ( 1 ) , thymidine ( 1 )

Downstream Regulation
Effects of modification on MAD1L1:
molecular association, regulation ( 1 , 3 ) , phosphorylation ( 1 ) , protein conformation ( 3 )
Effects of modification on biological processes:
carcinogenesis, induced ( 1 ) , cell cycle regulation ( 3 ) , DNA repair, induced ( 1 ) , signaling pathway regulation ( 1 )
Induce interaction with:
Ku80 (human) ( 1 ) , Mad2 (human) ( 3 )

References 

1

Xiao M, et al. (2020) Kinetochore protein MAD1 participates in the DNA damage response through ataxia-telangiectasia mutated kinase-mediated phosphorylation and enhanced interaction with KU80. Cancer Biol Med 17, 640-651
32944396   Curated Info

2

Bauer M, et al. (2015) Assessment of current mass spectrometric workflows for the quantification of low abundant proteins and phosphorylation sites. Data Brief 5, 297-304
26550600   Curated Info

3

Yang C, et al. (2014) ATM-mediated Mad1 Serine 214 phosphorylation regulates Mad1 dimerization and the spindle assembly checkpoint. Carcinogenesis 35, 2007-13
24728176   Curated Info

4

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

5

Zhou J (2011) CST Curation Set: 11707; Year: 2011; Biosample/Treatment: cell line, HeLa/UV; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]Q Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) ATM/ATR Substrate (S*/T*QG) (P-S/T2-100) Rabbit mAb Cat#: 6966, PTMScan(R) Phospho-ATM/ATR Substrate Motif (S*/T*QG) Immunoaffinity Beads Cat#: 6969
Curated Info

6

Zhou J (2011) CST Curation Set: 11708; Year: 2011; Biosample/Treatment: cell line, HeLa/UV; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]Q Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) ATM/ATR Substrate (S*/T*QG) (P-S/T2-100) Rabbit mAb Cat#: 6966, PTMScan(R) Phospho-ATM/ATR Substrate Motif (S*/T*QG) Immunoaffinity Beads Cat#: 6969
Curated Info

7

Matsuoka S, et al. (2007) ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. Science 316, 1160-6
17525332   Curated Info